SSR-Based Molecular Identification and Population Structure Analysis for Forage Pea (Pisum sativum var. arvense L.) Landraces

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dc.contributor.authorHaliloglu, Kamil
dc.contributor.authorTurkoglu, Aras
dc.contributor.authorTan, Mustafa
dc.contributor.authorPoczai, Peter
dc.date.accessioned2024-02-23T14:35:09Z
dc.date.available2024-02-23T14:35:09Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractPlant genetic diversity has a significant role in providing traits that can help meet future challenges, such as the need to adapt crops to changing climatic conditions or outbreaks of disease. Our aim in this study was to evaluate the diversity of 61 forage pea specimens (P. sativum ssp. arvense L.) collected from the northeastern Anatolia region of Turkey using 28 simple sequence repeat (SSR) markers. These primers generated a total of 82 polymorphic bands. The number of observed alleles (Na) per primer varied from 2 to 4 with a mean of 2.89 alleles/locus. The mean value of expected heterozygosity (Exp-Het = 0.50) was higher than the mean value of observed heterozygosity (Obs-Het = 0.22). The mean of polymorphic information content (PIC) was 0.41 with a range of 0.03-0.70. The mean number of effective alleles (Ne) was found to be 2.15, Nei's expected heterozygosity (H) 0.49, and Shannon's information index (I) 0.81. Cluster analysis through the unweighted pair-group mean average (UPGMA) method revealed that 61 forage pea landraces were divided into three main clusters. Genetic dissimilarity between the genotypes, calculated with the use of NTSYS-pc software, varied between 0.10 (G30 and G34) and 0.66 (G1 and G32). Principal coordinate analysis (PCoA) revealed that three principal coordinates explained 51.54% of the total variation. Moreover, population structure analysis showed that all genotypes formed three sub-populations. Expected heterozygosity values varied between 0.2669 (the first sub-population) and 0.3223 (third sub-population), with an average value of 0.2924. Average population differentiation measurement (Fst) was identified as 0.2351 for the first sub-population, 0.3838 for the second sub-population, and 0.2506 for the third sub-population. In general, current results suggest that SSR markers could be constantly used to illuminate the genetic diversity of forage pea landraces and can potentially be incorporated into future studies that examine the diversity within a larger collection of forage pea genotypes from diverse regions.en_US
dc.description.sponsorshipiASK Research Grant; Helsinki University Libraryen_US
dc.description.sponsorshipP.P. expresses his gratitude for the support of the iASK Research Grant. The authors thank the Helsinki University Library for supporting open-access publication.en_US
dc.identifier.doi10.3390/genes13061086
dc.identifier.issn2073-4425
dc.identifier.issue6en_US
dc.identifier.pmid35741848en_US
dc.identifier.scopus2-s2.0-85132680931en_US
dc.identifier.urihttps://doi.org/10.3390/genes13061086
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15906
dc.identifier.volume13en_US
dc.identifier.wosWOS:000819044400001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofGenesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGeneticen_US
dc.subjectMolecular Markersen_US
dc.subjectStructureen_US
dc.subjectUpgmaen_US
dc.titleSSR-Based Molecular Identification and Population Structure Analysis for Forage Pea (Pisum sativum var. arvense L.) Landracesen_US
dc.typeArticleen_US

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