Electrochemical biosensor based on three components random conjugated polymer with fullerene (C60)

Basit Öğe Kaydı
dc.contributor.authorKurbanoglu, Sevinc
dc.contributor.authorCevher, Sevki Can
dc.contributor.authorToppare, Levent
dc.contributor.authorCirpan, Ali
dc.contributor.authorSoylemez, Saniye
dc.date.accessioned2024-02-23T14:02:13Z
dc.date.available2024-02-23T14:02:13Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractHerein, a conjugated polymer and fullerene bearing architecture-based electrochemical Tyrosinase (Tyr) enzyme inhibition biosensor for indomethacin (INDO) drug active compound has been developed. For this purpose, three moieties of benzoxadiazole, thienopyrroledione, and benzodithiophene containing conjugated polymer; poly [BDT-alt-(TP;BO)] was used as a transducer modifier together with fullerene for catechol detection. The specific combination of these materials is considered an effective way to fabricate highly sensitive and fast response catechol biosensors for the first time. Electrochemical and surface characteristics of the modified electrodes were obtained by cyclic voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy, and atomic force microscopy. The effect of the parameters during chronoamperometric measurements on the biosensor response was also studied. Using optimized conditions, biosensing of catechol was achieved between 0.5 and 62.5 mu M with a limit of the detection 0.11 mu M. Tyr inhibition was followed with INDO drug active compound and it was found that INDO has a mixed type characteristic of enzyme kinetics with an I50 value of 15.11 mu M.en_US
dc.identifier.doi10.1016/j.bioelechem.2022.108219
dc.identifier.issn1567-5394
dc.identifier.issn1878-562X
dc.identifier.pmid35933973en_US
dc.identifier.scopus2-s2.0-85135417136en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.bioelechem.2022.108219
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11634
dc.identifier.volume147en_US
dc.identifier.wosWOS:000879859500002en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Saen_US
dc.relation.ispartofBioelectrochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCatechol Detectionen_US
dc.subjectEnzyme Inhibitionen_US
dc.subjectConjugated Random Polymeren_US
dc.subjectFullereneen_US
dc.subjectIndomethacinen_US
dc.subjectTyrosinaseen_US
dc.titleElectrochemical biosensor based on three components random conjugated polymer with fullerene (C60)en_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu