Comparison between liquid chromatography-tandem mass spectrometry and immunoassay methods for measurement of plasma 25 (OH) vitamin D

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dc.contributor.authorKader, Saadet
dc.contributor.authorAkdag, Turan
dc.contributor.authorEcer, Busra
dc.contributor.authorAbusoglu, Sedat
dc.contributor.authorUnlu, Ali
dc.date.accessioned2024-02-23T14:29:52Z
dc.date.available2024-02-23T14:29:52Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractObjectives Vitamin D is one of the major hormones involved in the metabolism of calcium (Ca) and phosphorus (P). In the present study, we aimed to determine the analytical performance of the immunoassay method used for determining plasma 25-hydroxyvitamin D [25(OH)D] levels in routine clinical practice in laboratories. Methods Venous blood samples were collected from 156 patients for the comparisons and were analyzed with Siemens ADVIA Centaur XPT, the Roche Cobas 6,000's module e601, Abbott Architect i2000, and the liquid chromatography with tandem mass spectrometry (LC-MS/MS). Results The four methods were analyzed and compared through the Passing-Bablok regression for 25(OH)D, and the highest correlation was found at LC-MS/MS and Cobas 6,000's module e601 (r=0.799), LC-MS/MS/Abbott Architect i2000, and LC-MS/MS/Siemens ADVIA Centaur XPT as r=0.736, 0.721, respectively. The correlation coefficient was found between Abbott Architect i2000 with Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.934 and r=0.907, respectively. Also, the correlation coefficient was found between Roche Cobas e601 and Siemens ADVIA Centaur XPT as r=0.906. Conclusions The Roche Cobas assay showed better performance, compared with the other assays. Based on our findings, the chemiluminescence methods in automated systems seem to be expedient.en_US
dc.identifier.doi10.1515/tjb-2021-0105
dc.identifier.endpage718en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue6en_US
dc.identifier.scopus2-s2.0-85141192959en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage710en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2021-0105
dc.identifier.urihttps://hdl.handle.net/20.500.12452/14905
dc.identifier.volume47en_US
dc.identifier.wosWOS:000870822500001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal Of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subject25 (Oh)Den_US
dc.subjectAssayen_US
dc.subjectChemiluminescenceen_US
dc.subjectImmunoassay Methodsen_US
dc.subjectLiquid Chromatography With Tandem Mass Spectrometryen_US
dc.titleComparison between liquid chromatography-tandem mass spectrometry and immunoassay methods for measurement of plasma 25 (OH) vitamin Den_US
dc.typeArticleen_US

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