EFFICACY OF IN VITRO PROPAGATED COONTAIL (CERATOPHYLLUM DEMERSUM L.) ON QUALITY OF DIFFERENT WATER SAMPLES
Küçük Resim Yok
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Parlar Scientific Publications (P S P)
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Coontail (Ceratophyllum demersum L.) is an important aquatic macrophyte used for phytoremediation of heavy metals. Shoot tip, 1st and 2nd nodal meristem explants were cultured on Murashige and Skoog (MS) medium containing 0.05-0.40 mg/L 6-benzylaminopurine (BA) + 0.10 mg/L a-Naphthalene acetic acid (NAA). Shoot regeneration frequency ranged 83.33-91.67% and 58.33-83.33% and 50.00-91.67 % respectively for of shoot tip, 1st and 2nd nodal meristem explant. Number of shoots per explant ranged 10.33-29.53, 16.25-26.89 and 12.42-24.66 for shoot tip, 1st and 2nd stem nodes respectively. Maximum number of 29.53 and 26.89 shoots per explant of shoot tip and 1st stem node were obtained from MS medium containing 0.10 mg/L BA+ 0.10 mg/L NAA. Whereas, maximum of (24.66) shoots per explant from 2nd stem node were scored on MS medium with 0.05 mg/L BA+0.10 mg/L NAA. Mean shoot length for shoot tip, 1st and 2nd stem node explants ranged 0.50-1.49 cm, 0.94-1.93 cm and 0.68-1.68 cm respectively. In vitro regenerated plants significantly affected the water quality of water samples. Water conductivity and hardness of dam water increased after 15 and 30 days. While water conductivity and hardness of tap water decreased after 15 and 30 days. Water conductivity of drinking water decreased after 15 and 30 days with no change in hardness. pH of all water samples increased after 15 and 30 days. Only drinking water had high Al, Fe and Cu concentrations and Al concentration decreased from 51.0 mu g/L to below 20 mu g/L, Fe concentration decreased from 372.0 mu g/L standard level of <50.0 mu g/L and Cu concentration in public water was very high (360 mu g/L) and decreased rapidly to below 0.02 mu g/L within 15 days (S2) after culture of in vitro grown plants. The study suggests the possible and efficient use of in vitro grown coontails plants for phytoremediation studies.
Açıklama
Anahtar Kelimeler
Heavy Metals, In Vitro, Stem Node Explants, Water Quality
Kaynak
Fresenius Environmental Bulletin
WoS Q Değeri
Q4
Scopus Q Değeri
Cilt
25
Sayı
12
