Detection of the of Effects of Hexavalent Chromium by Histopathological and Electrophoretic Methods on Capoeta capoeta (Guldenstaedt 1773) and Squalius cephalus (Linnaeus 1758)
Küçük Resim Yok
Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Kafkas Univ, Veteriner Fakultesi Dergisi
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this study, effects of hexavalent chromium (CrVI) (as potassium dichromate) on Capoeta capoeta and Squalius cephalus obtained from Kars Creek were investigated by histopathological and electrophoretic methods. Fish were adapted into the medium for 10 days. Then three groups were made in which for each fish species ten fish were included. The fish in the 1st group were held in normal water, 2nd and 3rd groups were held in the water containing 10 mg/L CrVI, respectively for 10 and 20 days. At the end of this period, tissue and blood samples were taken. These samples were analyzed subsequently. After histopathological examination occasional necrosis, hydropic and vacuolar degenerations in liver of the fish were found which were exposure to 10 mg/L CrVI. An increase in the level of degeneration was observed in the livers tissues of the experimental fish groups in parallel to the increase of the duration. In SDS-PAGE of serum proteins, compared to control, slight thickening of some protein bands were detected in the Capoeta capoeta exposed to CrVI for 10 days. There were no significant changes in protein bands of Squalius cephalus. Thinning of some protein bands and a significant thickening of some protein bands in the Capoeta capoeta. Slight thickening of some protein bands were detected in the Squalius cephalus exposed to hexavalent chromium for 20 days. In exposure to depending on the time of hexavalent chromium on Capoeta capoeta and Squalius cephalus, it is concluded that liver tissue and serum protein expressions of these fish were increasingly degenerated.
Açıklama
Anahtar Kelimeler
Capoeta Capoeta, Squalius Cephalus, Chromiumvi, Sds-Page, Degeneration Of Liver
Kaynak
Kafkas Universitesi Veteriner Fakultesi Dergisi
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
19
Sayı
6
