HIGH FREQUENCY AXILLARY SHOOTS INDUCTION IN GRASS PEA (LATHYRUS SATIVUS L.)

Küçük Resim Yok

Tarih

2017

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

Bangladesh Botanical Soc

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Grass pea (Lathyrus sativus L.) is highly recalcitrant and difficult to regenerate under in vitro conditions. An efficient and reliable protocol was developed for induction of effective in vitro regeneration of grass pea using embryos with two cotyledons. The maximum shoot regeneration frequency of 89.80% and number of 15.83 shoots per explant was induced on MS medium containing 2 mg/l BAP and 1 mg/l NAA. The mean shoot length decreased proportionately with each increase in TDZ concentration irrespective of the IBA. The regenerated shoots produced roots on MS medium containing 0.5, 1.0 and 2.0 mg/l IBA. However, rooted plantlets flowered in the rooting media.

Açıklama

Anahtar Kelimeler

Axillary Shoots, Grass Pea, Mature Seed Embryo, Regeneration

Kaynak

Bangladesh Journal Of Botany

WoS Q Değeri

Q4

Scopus Q Değeri

Q4

Cilt

46

Sayı

1

Künye