Effects of royal jelly on the antisenescence, mitochondrial viability and osteogenic differentiation capacity of umbilical cord-derived mesenchymal stem cells

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dc.contributor.authorCicek, Guelsemin
dc.contributor.authorBagci, Fatma Oz
dc.date.accessioned2024-02-23T13:43:49Z
dc.date.available2024-02-23T13:43:49Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractMesenchymal stem cells (MSCs) are multipotent cells that have the ability to self-renew and regulate paracrine signalling and immune system processes. MSCs have extensive clinical applications in regeneration, functional reconstruction and cellular therapies. However, studies are needed to discover ways to improve the properties of MSCs, such as differentiation, and prevent senescence in culture, which are both very important for cell therapies. Royal jelly (RJ) is a nutritional substance produced by worker bees that contains a substantial amounts of proteins that are beneficial for cell growth and proliferation. RJ is widely used in traditional medicine today, and due to the specific components in its content, it has been reported to have antioxidant, antiproliferative, antimicrobial, neuroprotective, anti-inflammatory, immunomodulatory and anti-ageing properties. In our study, human Wharton's jelly mesenchymal stem cells (WJ-MSCs) derived from umbilical cord matrix were grown in culture medium supplemented with RJ. The control group comprised minimum essential medium (MEM) and 10% foetal bovine serum (FBS); RJ groups were formed using MEM, 10% FBS and 0.075 mg/ml or 0.150 mg/ml RJ. In our study, we evaluated the effect of RJ on WJ-MSC growth by MTT assay, proliferating cell nuclear antigen ELISA, beta-galactosidase activity assay, MitoTracker Green staining and differentiation tests in adipogenic, osteogenic and chondrogenic cell lines. It was observed that the number of mitochondria increased, senescence decreased and osteogenic differentiation increased after differentiation induction after the addition of RJ to MSC culture. In general, the results of this study indicate that WJ-MSCs enhance mitochondrial numbers and important cellular activities, such as antisenescence and osteogenic differentiation, and with increasing evidence from further studies, RJ supplementation may be found beneficial for the use of MSCs in bone engineering regenerative medicine or cell therapy.en_US
dc.description.sponsorshipThis research was supported by the Necmettin Erbakan University Scientific Research Unit (project number: 221218010). [221218010]; Necmettin Erbakan University Scientific Research Uniten_US
dc.description.sponsorshipThis research was supported by the Necmettin Erbakan University Scientific Research Unit (project number: 221218010).en_US
dc.identifier.doi10.1007/s00418-023-02243-z
dc.identifier.issn0948-6143
dc.identifier.issn1432-119X
dc.identifier.pmid37814144en_US
dc.identifier.scopus2-s2.0-85173938437en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1007/s00418-023-02243-z
dc.identifier.urihttps://hdl.handle.net/20.500.12452/10936
dc.identifier.wosWOS:001078083300001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofHistochemistry And Cell Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectRoyal Jellyen_US
dc.subjectMesenchymal Stem Cellsen_US
dc.subjectMitochondrial Activityen_US
dc.subjectOsteogenic Differentiationen_US
dc.titleEffects of royal jelly on the antisenescence, mitochondrial viability and osteogenic differentiation capacity of umbilical cord-derived mesenchymal stem cellsen_US
dc.typeArticleen_US

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