A barcode-DNA analysis method for the identification of plant oil adulteration in milk and dairy products

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dc.contributor.authorUncu, Ayse Ozgur
dc.contributor.authorUncu, Ali Tevfik
dc.date.accessioned2024-02-23T14:02:56Z
dc.date.available2024-02-23T14:02:56Z
dc.date.issued2020
dc.departmentNEÜen_US
dc.description.abstractIn the present work, a barcode-DNA analysis method is described for the detection of plant oil adulteration in milk and dairy products. The method relies on the fact that plant DNA should not be present in readily detectable amounts in a dairy product unless it contains undeclared plant material. Thus, a universal plant barcode is chosen as the target to be amplified from dairy samples. Accordingly, barcode PCR-CE (PCR-capillary electrophoresis) assays are described, which do not require preliminary information on the species source of the adulterant oil type. Two PCR-CE assays, one operating on the plastid trnL (UAA) intron and the other targeting its inner P6 loop in nested format, were shown to detect corn, soybean, rapeseed and sunflower oils in clarified butter, milk and yogurt. Both barcodes are robustly amplified with extremely conserved primers. While the intron provides the species discrimination ability, the P6 loop provides superior detection sensitivity.en_US
dc.identifier.doi10.1016/j.foodchem.2020.126986
dc.identifier.issn0308-8146
dc.identifier.issn1873-7072
dc.identifier.pmid32407998en_US
dc.identifier.scopus2-s2.0-85084332436en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.foodchem.2020.126986
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11909
dc.identifier.volume326en_US
dc.identifier.wosWOS:000537547200040en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofFood Chemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectChloroplast Genomeen_US
dc.subjectDna Barcodeen_US
dc.subjectFood Authenticityen_US
dc.subjectOrganelle Genomeen_US
dc.subjectPcren_US
dc.titleA barcode-DNA analysis method for the identification of plant oil adulteration in milk and dairy productsen_US
dc.typeArticleen_US

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