Efficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Method

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dc.contributor.authorSaylan, Aslihan
dc.contributor.authorDuman, Selcuk
dc.date.accessioned2024-02-23T14:48:46Z
dc.date.available2024-02-23T14:48:46Z
dc.date.issued2016
dc.departmentNEÜen_US
dc.description.abstractObjective: In 2014, enrolled 20 patients who applied to the Unit of Assisted Reproduction Techniques, Konya Necmettin Erbakan University. Based on the presence of hyaluronic acid (HA) in the oocyte-cumulus cell complex, sperm attached to HA in vivo were modeled in vitro. Available healthy sperm obtained in the swim-up procedure using HA were investigated. Materials and Methods: This observational cohort study, a routine analysis was conducted on the ejaculation samples obtained from 20 patients. We divided each sample into two groups and the swim-up method was applied. Human serum albumin (HSA, 0.5%) was added to samples from the first group. HA (10%) was added to samples from the second group. We determined the floating linear and non-linear sperm concentrations of both groups annexin V was used to determine the rate of apoptosis of these sperm. Results: Following swim-up, linear and non-linear sperm concentrations were higher in the group that contained HA compared to the group with HSA. However, there was a significantly higher apoptosis rate in the HSA group compared to the HA group. Conclusion: The addition of HA to the medium in the swim-up procedure positively affected sperm parameters. Thus, healthier sperm cells were obtained without DNA damage and with high motility.en_US
dc.identifier.endpage88en_US
dc.identifier.issn2228-5806
dc.identifier.issn2228-5814
dc.identifier.issue1en_US
dc.identifier.pmid27054122en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage83en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12452/17831
dc.identifier.volume18en_US
dc.identifier.wosWOS:000374246600011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherRoyan Insten_US
dc.relation.ispartofCell Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnnexin Ven_US
dc.subjectApoptosisen_US
dc.subjectHyaluronic Aciden_US
dc.subjectHumanen_US
dc.titleEfficacy of Hyaluronic Acid in The Selection of Human Spermatozoa with Intact DNA by The Swim-up Methoden_US
dc.typeArticleen_US

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