Determination of Autophagy in Human Cervicovaginal Smears by Cytological and Immunocytochemical Methods

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dc.contributor.authorTurkmen, Merve Ozcan
dc.contributor.authorDemirezen, Sayeste
dc.contributor.authorBeksac, Mehmet Sinan
dc.date.accessioned2024-02-23T14:37:53Z
dc.date.available2024-02-23T14:37:53Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractBackground: Autophagy is a catabolic process whereby organelles and long-lived proteins are recycled through lysosomes to maintain cellular homeostasis. This process is being widely studied using culture techniques and animal models; however, cervicovaginal smears have not been used to detect autophagy. Aims: Our study aims to detect and evaluate autophagy in normal, malignant, infectious, and atypical cells in cervicovaginal smears by using cytological and immunocytochemical methods. Materials and Methods: Papanicolaou-stained 200 cervicovaginal smears were examined and 55 of 200 (27.5%) smears containing negative for intraepithelial lesion or malignancy (NILM) with identifiable infections and/or reactive/reparative changes (INF); briefly, NILM-INF (n = 31, 56.4%), atypical (n = 4, 7.3%), and malignant cells (n = 20, 36.3%) were evaluated as a study group. One hundred forty-five of 200 (72.5%) normal smears were accepted as the NILM without any identifiable infections (control group). The autophagy marker protein Microtubule-associated protein 1 light chain 3 A (MAP1LC3A) was used for immunocytochemical examination. Results: The staining intensity of the MAP1LC3A protein and autophagy positivity were lower in the malignant cells; however, they were higher in the NILM-INF and atypical cells. A statistically significant correlation between the malignant and normal cells was obtained for the autophagy positivity (P = 0.012). In view of the staining intensity of MAP1LC3A protein by the H-score method, a significant correlation was found between the NILM-INF and the normal cells (P = 0.015). Conclusions: Autophagy was detected in various cervicovaginal smears for the first time in this study. Our findings indicate that an autophagy process is essential in infectious cells as well as in the transformation of atypical cells into malignant cells in carcinogenesis.en_US
dc.description.sponsorshipTeaching Staff Training Program (OYP) of Hacettepe Universityen_US
dc.description.sponsorshipThis study was supported by Teaching Staff Training Program (OYP) of Hacettepe University.en_US
dc.identifier.doi10.4103/joc.joc_130_22
dc.identifier.endpage183en_US
dc.identifier.issn0970-9371
dc.identifier.issn0974-5165
dc.identifier.issue4en_US
dc.identifier.pmid38058675en_US
dc.identifier.scopus2-s2.0-85176344336en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage177en_US
dc.identifier.urihttps://doi.org/10.4103/joc.joc_130_22
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16262
dc.identifier.volume40en_US
dc.identifier.wosWOS:001107363900005en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherWolters Kluwer Medknow Publicationsen_US
dc.relation.ispartofJournal Of Cytologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAutophagyen_US
dc.subjectCervicovaginal Smearen_US
dc.subjectImmunocytochemistryen_US
dc.subjectMap1lc3aen_US
dc.titleDetermination of Autophagy in Human Cervicovaginal Smears by Cytological and Immunocytochemical Methodsen_US
dc.typeArticleen_US

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