The Role of Stem Cells Derived From the Mesenchyme of the Umbilical Cord in Reducing Immunosuppressive Drug Doses Used in Allogenic Transplantations

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dc.contributor.authorEvin, Seyda Guray
dc.contributor.authorSutcu, Mustafa
dc.contributor.authorAktan, Tahsin Murad
dc.contributor.authorDuman, Selcuk
dc.contributor.authorHarmankaya, Ismail
dc.contributor.authorAbusoglu, Sedat
dc.date.accessioned2024-02-23T14:23:27Z
dc.date.available2024-02-23T14:23:27Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractBackgroundThis study evaluated the potential of Wharton's jelly mesenchymal stem cells with high tolerogenic properties in reducing immunosuppressive dosage and related adverse effects.MethodsA 4- to 6-week-old, 30-40 g weight, male inbred CD57BL/6 mice were used as skin allograft donors, whereas Balb/c mice with similar characteristics were used as recipients. Wharton's jelly stem cells were obtained from a commercial kit sourced from human umbilical cord. Skin allografts were performed from CD57Bl6 to Balb/c mice (day 0). Group 1 (control) received no treatment. Group 2 received 15 mg/kg cyclosporin A on days 0 to 30. Group 3 received 5.7 x 10(6) and 10.3 x 10(6) cell/kg Wharton's jelly stem cells on days 0 and 3, respectively. Groups 4, 5, and 6 received a combination of 15, 10, and 5 mg/kg per day cyclosporine A (days 0 to 30) with the same stem cell dose with group 3, respectively. Graft rejection was evaluated with digital photography and thermal imaging, histopathology (Banff grading, epithelialization scores, dermoepidermal dissociation), immunochemistry (Ki-67 and Bcl-2), and biochemical methods (interleukin 10, interleukin 2, interferon gamma, tumor necrosis factor alpha) (day 10). Cumulative adverse effects of cyclosporin A occurring in the groups were revealed by histopathological evaluation of kidney and liver (a modified semiquantitative method of infiltration of inflammatory cells around the portal area and lobular region in liver; modification of the Banff rating of proximal tubules and hypertrophia of juxtaglomerular apparatus cells in kidney) (day 30).ResultsThere was no rejection in groups 2, 4, and 5 until the end of study. These were statistically different versus groups 1 (day 10 +/- 0.71), 3 (day 11 +/- 0.82), and 6 (day 11 +/- 0.58) (all P's < 0.05). Groups 4 and 5 have exhibited statistically similar findings in histopathological (4 epithelization score: 3.7 +/- 1.3; 5 epithelization score: 3.5 +/- 0.5; 4 Banff grading score: 0.8 +/- 0.6; 5 Banff grading score: 1.0 +/- 0.5; both P's = 1.00), immunohistochemical (4 Bcl-2 score: 3.5 +/- 0.5, P = 0.618; 5 Bcl-2 score: 3.4 +/- 0.5, P = 1.00; 4 Ki-67 score: 3.7 +/- 0.4, P = 1.00; 5 Ki-67 score: 3.5 +/- 0.5, both P's = 1.00), and levels of cytokines (both P's = 1.00) versus group 2. Adverse effects on kidneys and liver were lowest and statistically similar in groups 3, 5, and 6 (all P's = 00) versus group 1.ConclusionsWharton's jelly mesenchymal stem cells alter bioavailability of cyclosporine, albeit at much lower doses and with fewer systemic adverse effects.en_US
dc.identifier.doi10.1097/SAP.0000000000003314
dc.identifier.endpage693en_US
dc.identifier.issn0148-7043
dc.identifier.issn1536-3708
dc.identifier.issue6en_US
dc.identifier.pmid36416704en_US
dc.identifier.scopus2-s2.0-85142939181en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage684en_US
dc.identifier.urihttps://doi.org/10.1097/SAP.0000000000003314
dc.identifier.urihttps://hdl.handle.net/20.500.12452/13550
dc.identifier.volume89en_US
dc.identifier.wosWOS:000891172800029en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherLippincott Williams & Wilkinsen_US
dc.relation.ispartofAnnals Of Plastic Surgeryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAllotransplantationen_US
dc.subjectAdverse Effects Of Immunosuppressanten_US
dc.subjectWharton's Jelly Mesenchymal Stem Cellen_US
dc.titleThe Role of Stem Cells Derived From the Mesenchyme of the Umbilical Cord in Reducing Immunosuppressive Drug Doses Used in Allogenic Transplantationsen_US
dc.typeArticleen_US

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