Anticancer Effects of Vitis vinifera L. Mediated Biosynthesized Silver Nanoparticles and Cotreatment with 5 Fluorouracil on HT-29 Cell Line

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dc.contributor.authorSalman, Giray
dc.contributor.authorPehlivanoglu, Suray
dc.contributor.authorAcar, Cigdem Aydin
dc.contributor.authorYesilot, Sukriye
dc.date.accessioned2024-02-23T13:59:43Z
dc.date.available2024-02-23T13:59:43Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractThe aim of this study was to evaluate the anticancer effects of biosynthesized silver nanoparticles (Vv-AgNPs) from grape (Vitis vinifera L.) seed aqueous extract, alone or in combination with 5-Fluorouracil (5-FU) on HT-29 cell line. Vv-AgNPs were characterized by techniques such as UV-vis spectrophotometer (surface plasmon peak 454 nm), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). HT-29 cells were treated with different concentrations (0-80 mu g/mL for MTT) and (0-20 mu g/mL for BrdU) of Vv-AgNPs alone and combined with (200 mu g/mL) 5-FU for 72 h. The cytotoxic effects were analyzed by [3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyl tetrazolium bromide] (MTT) assay (IC50 values 13.74 and 5.35 mu g/mL, respectively). Antiproliferative effects were examined 5-bromo-2'-deoxyuridine (BrdU) assay (IC50 values 9.65 and 5.00 mu g/mL, respectively). Activation of caspase-3 and protein expression levels of p53 were determined by Western blotting analysis. It was observed that Vv-AgNPs significantly increased the cleavage of the proapoptotic proteins caspase 3 and obviously enhanced the expression of p53 in a dose-dependent manner. The increased amount of total oxidant status (TOS) in the 10 mu g/mL Vv-AgNPs + 5-FU treatment group, despite the increasing amount of total antioxidant status (TAS), caused an increase in Oxidative Stress Index (OSI) compared to the control. In this study, it has been shown in vitro that the use of successfully biosynthesized Vv-AgNPs in combination with 5-FU exhibits synergistic cytotoxic, antiproliferative, apoptotic, and oxidative effects against HT-29 cell line.en_US
dc.description.sponsorshipBurdur Mehmet Akif Ersoy University Scientific Research Projects Unit [0594-YL-19]en_US
dc.description.sponsorshipThis research was supported by the Burdur Mehmet Akif Ersoy University Scientific Research Projects Unit [Project number: 0594-YL-19].en_US
dc.identifier.doi10.1007/s12011-021-02923-8
dc.identifier.endpage3170en_US
dc.identifier.issn0163-4984
dc.identifier.issn1559-0720
dc.identifier.issue7en_US
dc.identifier.pmid34546492en_US
dc.identifier.scopus2-s2.0-85115256838en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage3159en_US
dc.identifier.urihttps://doi.org/10.1007/s12011-021-02923-8
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11295
dc.identifier.volume200en_US
dc.identifier.wosWOS:000697609300001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringernatureen_US
dc.relation.ispartofBiological Trace Element Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGreen Synthesisen_US
dc.subjectSilver Nanoparticlesen_US
dc.subject5-Fluorouracilen_US
dc.subjectHt-29 Cell Lineen_US
dc.subjectColon Canceren_US
dc.titleAnticancer Effects of Vitis vinifera L. Mediated Biosynthesized Silver Nanoparticles and Cotreatment with 5 Fluorouracil on HT-29 Cell Lineen_US
dc.typeArticleen_US

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