Cigarette smoke and bleomycin-induced pulmonary oxidative stress in rats

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dc.contributor.authorTeke, Turgut
dc.contributor.authorMaden, Emin
dc.contributor.authorKiyici, Aysel
dc.contributor.authorKorkmaz, Celalettin
dc.contributor.authorGok, Mehmet
dc.contributor.authorOzer, Faruk
dc.contributor.authorImecik, Oktay
dc.date.accessioned2024-02-23T14:35:26Z
dc.date.available2024-02-23T14:35:26Z
dc.date.issued2012
dc.departmentNEÜen_US
dc.description.abstractBleomycin causes pulmonary fibrosis by increasing free oxygen radicals. Cigarette smoke is a strong oxidant which adversely affects pulmonary tissue. We evaluated the effects of cigarette smoke administered with intratracheal bleomycin on pulmonary tissue. We studied 3 groups of rats (n=10): one group received intratracheal saline and served as a control; one received intratracheal Neomycin (IT) (0.5 U/100 g body weight, single dose on the first day), and one group received intratracheal bleomycin (single dose on first day) and tobacco smoke (two times per day) (IT-S). After 4 weeks, the levels of malondialdehyde (MDA) and nitric oxide (NO) and the activities of superoxide dismutase (SOD) and xanthine oxidase (XO) were assayed in the homogenate of the lung tissue samples. The severity of interstitial fibrosis was assessed using the grading system described by Ashcroft. There was more intensive fibrosis in the IT and IT-S than in the control samples (P<0.001). The levels of MDA., NO and activity of XO were significantly increased (P<0.001, <0.002 and <0.002, respectively), and SOD activity (P<0.001) was significantly decreased in the IT group when compared to these values in the control group. The concentration of NO was significantly decreased (P<0.002), and SOD activity was significantly increased (P<0.05) in the lung tissue samples of the IT-S group. Theoretically, the combination of cigarette smoke and bleomycin may have a synergistic effect on oxidative lung injury. In conclusion, we showed that inhalation of cigarette smoke provides protection against oxidative stress in the lung tissue of rats with bleomycin-induced pulmonary fibrosis.en_US
dc.identifier.doi10.3892/etm.2012.550
dc.identifier.endpage124en_US
dc.identifier.issn1792-0981
dc.identifier.issue1en_US
dc.identifier.pmid23060934en_US
dc.identifier.scopus2-s2.0-84865709276en_US
dc.identifier.startpage121en_US
dc.identifier.urihttps://doi.org/10.3892/etm.2012.550
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16027
dc.identifier.volume4en_US
dc.identifier.wosWOS:000305046100021en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpandidos Publ Ltden_US
dc.relation.ispartofExperimental And Therapeutic Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBleomycinen_US
dc.subjectOxidative Stressen_US
dc.subjectPulmonary Fibrosisen_US
dc.subjectCigarette Smokeen_US
dc.titleCigarette smoke and bleomycin-induced pulmonary oxidative stress in ratsen_US
dc.typeArticleen_US

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