Investigation of Tomato Ringspot Virus (ToRSV) by Real-Time TaqMan RT-PCR in Hakkari Province, Turkey

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dc.contributor.authorAkdura, Nevin
dc.contributor.authorSevik, Murat
dc.date.accessioned2024-02-23T14:30:05Z
dc.date.available2024-02-23T14:30:05Z
dc.date.issued2021
dc.departmentNEÜen_US
dc.description.abstractTomato ringspot virus (ToRSV) belongs to the Nepovirus genus in the family Secoviridae. It has a wide host range and is listed as a quarantine virus in Turkey. In this study, 80 leaf samples were collected from tomato, pepper, cucumber and grapevine cultivation sites located in three different parts of Hakkari province: Semdinli, Cukurca and Center districts. Real-time TaqMan reverse transcription-polymerase chain reaction (RT-PCR) method was used for the detection of the virus. Amplification was carried out in reaction mix including QuantiNova Probe RT-PCR kit (Qiagen, Germany) using primers and TaqMan probe based on 3'-UTR (untranslated region) of virus, which amplified a 182 bp product of the genome. ToRSV was detected in 13 of the 80 samples and threshold cycle (CT) values ranged from 23.9 to 37.4. It was found that 16.25% of the samples collected from the districts of Hakkari province were found to be infected with ToRSV whereas no ToRSV was detected in the samples collected from the center of the city. The virus was detected on pepper and cucumber samples in Cukurca district, and it was also detected in tomato, pepper, cucumber and grapevine samples in Semdinli district. To our knowledge, this study is the first report of molecular detection of ToRSV by real-time TaqMan RT-PCR in Turkey.en_US
dc.description.sponsorshipScientific Research Projects Unit of Hakkari University [EF2015BAP4]en_US
dc.description.sponsorshipThe study was supported by a grant from Scientific Research Projects Unit of Hakkari University (Project no: EF2015BAP4). We are grateful to Dr. Stephan Winter, Dr. Wulf Menzel (Leibniz Institute, DSMZ, Germany) and Dr. Farshad Rakhshandehroo (Islamic Azad University, Tehran, Iran) for providing us ToRSV isolate as positive control and support.en_US
dc.identifier.doi10.15832/ankutbd.677710
dc.identifier.endpage340en_US
dc.identifier.issn1300-7580
dc.identifier.issn2148-9297
dc.identifier.issue3en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage335en_US
dc.identifier.urihttps://doi.org/10.15832/ankutbd.677710
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15008
dc.identifier.volume27en_US
dc.identifier.wosWOS:000695214900013en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofJournal Of Agricultural Sciences-Tarim Bilimleri Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectTorsven_US
dc.subjectMolecular Detectionen_US
dc.subjectTomatoen_US
dc.subjectPepperen_US
dc.subjectCucumberen_US
dc.subjectGrapevineen_US
dc.subjectTurkeyen_US
dc.titleInvestigation of Tomato Ringspot Virus (ToRSV) by Real-Time TaqMan RT-PCR in Hakkari Province, Turkeyen_US
dc.typeArticleen_US

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