Rosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stress

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dc.contributor.authorArikan, Busra
dc.contributor.authorOzfidan-Konakci, Ceyda
dc.contributor.authorAlp, Fatma Nur
dc.contributor.authorZengin, Gokhan
dc.contributor.authorYildiztugay, Evren
dc.date.accessioned2024-02-23T14:13:35Z
dc.date.available2024-02-23T14:13:35Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractThe impacts of exogenous rosmarinic acid (RA, 100 mu M) and/or hesperidin (HP, 100 mu M) were evaluated in improving tolerance on the gas exchange, chlorophyll fluorescence and efficiencies, phenomenological fluxes of photosystems, antioxidant system and gene expression related to the lipid biosynthesis under heat stress. For this purpose, Arabidopsis thaliana was grown under RA and HP with heat stress (S, 38 ?) for 24 h(h). As shown in gas exchange parameters, heat stress caused mesophyll efficiency and non-stomatal restrictions. Both alone and combined forms of RA and HP to stress-treated A. thaliana alleviated the disturbance of carbon assimilation, transpiration rate and internal CO2 concentrations. Stress impaired the levels of energy flow reaching reaction centers of PSII and the photon capture ability of active reaction centers. RA and/or HP enhanced photosystems' structural/functional characteristics and photosynthetic performance. Histochemical staining and biochemical analyses revealed that heat stress caused the oxidation in A. thaliana. By activating several defensive mecha-nisms, RA and/or HP could reverse the harm caused by radical production. Both alone and combined forms of RA and HP removed superoxide anion radical (O-2 & BULL;-) accumulation, inducing superoxide dismutase (SOD). The common enzyme that scavenged hydrogen peroxide (H2O2) at all three applications (S + RA, S + HP and S + RA + HP) was POX. Also, only RA could utilize the ascorbate (AsA) regeneration in response to stress, suggesting increased ascorbate peroxidase (APX), monodehydroascorbate (MDHAR) and dehydroascorbate (DHAR) activ-ities. However, the regeneration/redox state of AsA and glutathione (GSH) did not maintain under S + HP and S + RA + HP. While RA had no positive influence on the saturated fatty acids under stress, HP increased the total saturated fatty acids (primarily palmitic acid). Besides, the combined application of RA + HP effectively created the stress response by increasing the expression of genes involved in fatty acid synthesis. The synergetic in-teractions of RA and HP could explain the increased levels of saturated fatty acids in combining these com-pounds. The data obtained from the study will contribute to the responses of phenolic compounds in plants to heat stress.en_US
dc.description.sponsorshipSelcuk University [201315005]; Necmettin Erbakan University Scientific Research Projects Coordinating Office [20201038]en_US
dc.description.sponsorshipThis work was supported by Selcuk University and Necmettin Erbakan University Scientific Research Projects Coordinating Office (Grant Number: 20201038 and 201315005, respectively) .en_US
dc.identifier.doi10.1016/j.phytochem.2022.113157
dc.identifier.issn0031-9422
dc.identifier.issn1873-3700
dc.identifier.pmid35271935en_US
dc.identifier.scopus2-s2.0-85126117280en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.urihttps://doi.org/10.1016/j.phytochem.2022.113157
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12488
dc.identifier.volume198en_US
dc.identifier.wosWOS:000791302400010en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofPhytochemistryen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectArabidopsis Thalianaen_US
dc.subjectBrassicaceaeen_US
dc.subjectHesperidinen_US
dc.subjectRosmarinic Aciden_US
dc.subjectGene Expressionen_US
dc.subjectHeat Stressen_US
dc.titleRosmarinic acid and hesperidin regulate gas exchange, chlorophyll fluorescence, antioxidant system and the fatty acid biosynthesis-related gene expression in Arabidopsis thaliana under heat stressen_US
dc.typeArticleen_US

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