LC-ESI-QTOF-MS/MS Analysis, Cytotoxic, Antiviral, Antioxidant, and Enzyme Inhibitory Properties of Four Extracts of Geranium pyrenaicum Burm. f.: A Good Gift from the Natural Treasure

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dc.contributor.authorSwiatek, Lukasz
dc.contributor.authorSieniawska, Elwira
dc.contributor.authorSinan, Kouadio Ibrahime
dc.contributor.authorMaciejewska-Turska, Magdalena
dc.contributor.authorBoguszewska, Anastazja
dc.contributor.authorPolz-Dacewicz, Malgorzata
dc.contributor.authorSenkardes, Ismail
dc.date.accessioned2024-02-23T14:35:10Z
dc.date.available2024-02-23T14:35:10Z
dc.date.issued2021
dc.departmentNEÜen_US
dc.description.abstractThis study focused on the biological evaluation and chemical characterization of Geranium pyrenaicum Burm. f. Different solvent extracts (hexane, ethyl acetate, methanol, and water extracts) were prepared. The phytochemical profile, antioxidant, and enzyme inhibitory activity were investigated. Cytotoxicity was assessed using VERO, FaDu, HeLa and RKO cells. The antiviral activity was carried out against HSV-1 (Herpes simplex virus 1) propagated in VERO cell line. The aqueous extract, possessing high phenolic content (170.50 mg gallic acid equivalent/g extract), showed the highest reducing capacity (613.27 and 364.10 mg Trolox equivalent/g extract, for cupric reducing antioxidant capacity and ferric reducing antioxidant power, respectively), radical scavenging potential (469.82 mg Trolox equivalent/g extract, against 2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)), metal chelating ability (52.39 mg ethylenediaminetetraacetic acid equivalent/g extract) and total antioxidant capacity (3.15 mmol Trolox equivalent/g extract). Liquid chromatography-electrospray ionization-quadrupole time-of-flight-mass spectrometry (LC-ESI-QTOF-MS/MS) alloved to tentatively identify a total of 56 compounds in the extracts, including ellagitannins, gallic acid and galloyl derivatives amongst others. The ethyl acetate extracts substantially depressed cholinesterase enzymes (4.49 and 12.26 mg galantamine equivalent/g extract against AChE and BChE, respectively) and alpha-amylase enzyme (1.04 mmol acarbose equivalent/g extract). On the other hand, the methanolic extract inhibited tyrosinase (121.42 mg kojic acid equivalent/g extract) and alpha-glucosidase (2.39 mmol acarbose equivalent/g extract) activities. The highest selectivity towards all cancer cell lines (SI 4.5-10.8) was observed with aqueous extract with the FaDu cells being the most sensitive (CC50 40.22 mu g/mL). It can be concluded that the presence of certain bioactive antiviral molecules may be related to the high anti HSV-1 activity of the methanolic extract. This work has generated vital scientific data on this medicinal plant, which is a prospective candidate for the creation of innovative phyto-pharmaceuticals.en_US
dc.description.sponsorshipScientific Research Projects Foundation of Necmettin Erbakan University [201210005]en_US
dc.description.sponsorshipThis research was funded by the Scientific Research Projects Foundation of Necmettin Erbakan University (Project No: 201210005).en_US
dc.identifier.doi10.3390/ijms22147621
dc.identifier.issn1422-0067
dc.identifier.issue14en_US
dc.identifier.pmid34299238en_US
dc.identifier.scopus2-s2.0-85110212461en_US
dc.identifier.urihttps://doi.org/10.3390/ijms22147621
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15911
dc.identifier.volume22en_US
dc.identifier.wosWOS:000676843100001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofInternational Journal Of Molecular Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGeraniumen_US
dc.subjectAntioxidantsen_US
dc.subjectPolyphenolsen_US
dc.subjectEnzymesen_US
dc.subjectHsv-1en_US
dc.subjectCytotoxicityen_US
dc.titleLC-ESI-QTOF-MS/MS Analysis, Cytotoxic, Antiviral, Antioxidant, and Enzyme Inhibitory Properties of Four Extracts of Geranium pyrenaicum Burm. f.: A Good Gift from the Natural Treasureen_US
dc.typeArticleen_US

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