Pinealectomy alters IFN-? and IL-10 levels in primary thymocyte culture of rats

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dc.contributor.authorSahin, Zafer
dc.contributor.authorSandal, Suleyman
dc.contributor.authorYilmaz, Bayram
dc.contributor.authorBulmus, Ozgur
dc.contributor.authorOzdemir, Gokcen
dc.contributor.authorKutlu, Selim
dc.contributor.authorGodekmerdan, Ahmet
dc.date.accessioned2024-02-23T14:29:36Z
dc.date.available2024-02-23T14:29:36Z
dc.date.issued2018
dc.departmentNEÜen_US
dc.description.abstractMelatonin, produced mainly by the pineal gland, has an immunomodulatory role. However, the effects of the pineal gland and/or melatonin on thymus cytokine levels such as interferon-gamma (IFN-gamma), interleukin (IL)-4, and IL-10 are not well known. Twenty-one male Wistar rats (220-250 gr) were randomly divided into three groups (n=7): intact control, sham, and pinealectomy. Primary thymocyte cultures were prepared from each group and dispensed into well plates as Control, DMSO (or vehicle), Sham-pinealectomy, Pinealectomy, Pinealectomy+10 mu M melatonin, and Pinealectomy+100 mu M melatonin. IFN-gamma, IL-4, and IL-10 concentrations were measured in the thymocytes (as nonstimulated and Concanavalin A-stimulated) after 24 h. IFN-gamma levels significantly increased and IL-10 levels significantly decreased in both media prepared from pinealectomized rats. There was no significant difference between the groups in terms of IL-4. In the pinealectomy+100 mu M melatonin group, IFN-gamma and IL-10 levels did not differ from the pinealectomy group. However, the dose of 100 mu M melatonin caused a decrease in levels of IFN-gamma in both thymocyte media and an increase in the concentration of IL-10 in Concanavalin A-stimulated thymocytes. In conclusion, pineal gland and or melatonin affect IFN-gamma and IL-10 levels in the thymus gland.en_US
dc.description.sponsorshipResearch Foundation of Firat University [FUBAP-1101]en_US
dc.description.sponsorshipThis work was supported by the Research Foundation of Firat University (Project No: FUBAP-1101).en_US
dc.identifier.doi10.14715/cmb/2018.64.14.5
dc.identifier.endpage30en_US
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue14en_US
dc.identifier.pmid30511618en_US
dc.identifier.scopus2-s2.0-85060028255en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage25en_US
dc.identifier.urihttps://doi.org/10.14715/cmb/2018.64.14.5
dc.identifier.urihttps://hdl.handle.net/20.500.12452/14751
dc.identifier.volume64en_US
dc.identifier.wosWOS:000451905800005en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherC M B Assocen_US
dc.relation.ispartofCellular And Molecular Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectPineal Glanden_US
dc.subjectMelatoninen_US
dc.subjectCytokineen_US
dc.subjectThymocyteen_US
dc.subjectT Helperen_US
dc.subjectRaten_US
dc.titlePinealectomy alters IFN-? and IL-10 levels in primary thymocyte culture of ratsen_US
dc.typeArticleen_US

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