Brain-Derived Neurotrophic Factor Levels in Mesenchymal Stem Cell Culture Fluid Under Different Culture Conditions

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dc.contributor.authorCicek, Gulsemin
dc.contributor.authorCicek, Muharrem
dc.contributor.authorOzen, Emine Utlu
dc.contributor.authorAktan, T. Murad
dc.contributor.authorOzdengul, Faik
dc.contributor.authorDuman, Selcuk
dc.date.accessioned2024-02-23T14:41:05Z
dc.date.available2024-02-23T14:41:05Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractObjective: Mesenchymal stem cells are used in various fields, such as cellular therapy, regeneration, or tissue engineering. It has been shown that they exhibit many protective factors and also work as a modulating chief within the region in which they are administered. There are studies on both the therapeutic and neuroprotective effects of brain-derived neurotrophic factor. Also, there are many studies on the improvement of culture conditions for in vitro reproduction of mesenchymal stem cells, which can be obtained from many sources in various bodies, such as adipose tissue and Wharton's jelly. Improving and standardizing these culture conditions will increase the effectiveness and reliability of stem cell therapies. Studies evaluating many culture conditions, such as O-2 level, type of medium, monolayer culture, and the transition from in vitro 3D models, are ongoing. Materials and Methods: In our study, groups were formed by using stem cells originating from adipose tissue and Wharton's jelly. Stem cell cultures were made using Hillex-II and Pronectin-F microcarriers. Cell culture O-2 level was adjusted as 1% and 5% for each group separately. Enzyme-linked immunosorbent assay was used to analyze brain-derived neurotrophic factor levels in stem cell culture fluid. Results: The highest brain-derived neurotrophic factor level in mesenchymal stem cells culture medium was observed in an adipose-derived stem cell culture with an in vitro fertilization (non-treated) dish, using a Hillex microcarrier in a 1% O 2 microenvironment. Conclusion: As a result of our observations, we think that cells could exhibit greater therapeutic potential in a dynamic adhesion environment.en_US
dc.identifier.doi10.5152/eurasianjmed.2021.0138
dc.identifier.endpage31en_US
dc.identifier.issn1308-8742
dc.identifier.issue1en_US
dc.identifier.pmid36861862en_US
dc.identifier.scopus2-s2.0-85150014162en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage25en_US
dc.identifier.urihttps://doi.org/10.5152/eurasianjmed.2021.0138
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16702
dc.identifier.volume55en_US
dc.identifier.wosWOS:000992442400005en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAvesen_US
dc.relation.ispartofEurasian Journal Of Medicineen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMesenchymal Stem Cellen_US
dc.subjectCulture Fluiden_US
dc.subjectBrain-Derived Neurotrophic Factoren_US
dc.subjectHypoxiaen_US
dc.subjectMicrocarrieren_US
dc.titleBrain-Derived Neurotrophic Factor Levels in Mesenchymal Stem Cell Culture Fluid Under Different Culture Conditionsen_US
dc.typeArticleen_US

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