Tissue thioredoxin reductase-1 expression in astrocytomas of different grades

Basit Öğe Kaydı
dc.contributor.authorEsen, Hasan
dc.contributor.authorErdi, Fatih
dc.contributor.authorKaya, Bulent
dc.contributor.authorFeyzioglu, Bahadir
dc.contributor.authorKeskin, Fatih
dc.contributor.authorDemir, Lutfi Saltuk
dc.date.accessioned2024-02-23T13:59:24Z
dc.date.available2024-02-23T13:59:24Z
dc.date.issued2015
dc.departmentNEÜen_US
dc.description.abstractThioredoxin (Trx) is a redox active protein that regulates several physiological and biochemical functions, such as growth, apoptosis and cellular defense. The function of Trx itself is regulated by thioredoxin reductase (TrxR). Studies performed in a variety of human primary tumors have shown that thioredoxin reductase 1 (TrxR1) is overexpressed in tumoral tissues compared with corresponding normal tissues. This study was designed to determine the expression of TrxR1 in astrocytoma tissues of different World Health Organization (WHO) grades (grade I-IV). The proliferative (Ki-67) and apoptotic indices of the specimens were also investigated for correlation analysis. Astrocytoma tissues were extracted from the histopathological specimens of 40 patients. These samples included seven histologically normal brain tissues that served as a control group and ten tumoral samples for each grade of astrocytoma (grade I-IV). The histologically normal brain tissues were obtained from the non-tumoral portions of the pathological specimens of grade I (2 cases), grade II (2 cases), grade III (2 cases) and grade IV (1 case) astrocytomas. TrxR1 expression was evaluated using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and immunostaining. The proliferative and apoptotic indices of the specimens were investigated by Ki-67 immunostaining and TUNEL assay, respectively. TrxR1 expression, as assessed by qRT-PCR, increased significantly with astrocytoma grade (p = 0.01). The immunostaining intensity of TrxR1 in grade IV astrocytomas was significantly greater than that in the control tissue and all other astrocytoma grades (p < 0.001). Similarly, immunostaining intensity of TrxR1 in the grade III astrocytomas was significantly greater than that in the control group and grade I astrocytomas (p < 0.001). All astrocytoma tissues showed more intense staining in ascending grades, but the differences between grade I and the control, grade II and the control, grades II and I, grades III and II were not statistically significant (p > 0.05). Ki-67 index values increased significant in accordance with grade progression (p = 0.01). The apoptotic index values were not significantly different in any group (p > 0.05); however, the differences between grade IV and the control and between grades IV and I were statistically significant (p < 0.05). Expression of TrxR1, as assessed by both qRT-PCR and immunostaining, correlated highly with both the astrocytoma grade and Ki-67 index.en_US
dc.identifier.doi10.1007/s11060-014-1661-5
dc.identifier.endpage458en_US
dc.identifier.issn0167-594X
dc.identifier.issn1573-7373
dc.identifier.issue3en_US
dc.identifier.pmid25391969en_US
dc.identifier.scopus2-s2.0-84925491384en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage451en_US
dc.identifier.urihttps://doi.org/10.1007/s11060-014-1661-5
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11168
dc.identifier.volume121en_US
dc.identifier.wosWOS:000349527500004en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal Of Neuro-Oncologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAstrocytomaen_US
dc.subjectTrxr-1en_US
dc.subjectExpressionen_US
dc.subjectApoptosisen_US
dc.subjectProliferationen_US
dc.subjectGradeen_US
dc.titleTissue thioredoxin reductase-1 expression in astrocytomas of different gradesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayın Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu