Investigation of the interchromosomal effects in male carriers with structural chromosomal abnormalities using FISH

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dc.contributor.authorBalasar, Ozgur
dc.contributor.authorAcar, Hasan
dc.date.accessioned2024-02-23T14:41:17Z
dc.date.available2024-02-23T14:41:17Z
dc.date.issued2020
dc.departmentNEÜen_US
dc.description.abstractObjective: The interchromosomal effect (ICE) refers to the uncertainty during meiosis where the rearrangement of the chromosomes affects the segregation of the chromosomes that are not involved in the structural chromosomal abnormalities. The aim of this study is to investigate the existence of ICE in the sperm nuclei of the males who have structural chromosomal abnormalities. Material and methods: Nine male individuals who are the carriers of the structural chromosomal abnormalities (patient group) and 14 male individuals who did not have any chromosomal abnormalities (control group) were diagnosed by the classical cytogenetic analysis. The aneuploidy of chromosomes 2, 3, 12, 13, 17, 18, 21, X, and Y in the sperm nuclei was investigated using the fluorescence in situ hybridization (FISH) method in these individuals. The patient group included 5 Robertsonian translocation (ROB) carriers, 3 reciprocal translocation (RCP) carriers, and 1 inversion carrier. Results: A total of 51921 sperm nuclei were analyzed (19484 from the patient group and 32437 from the control group). While ICE was determined in 4 of 5 patients who were the carriers of ROB and an inversion carrier patient, it was not determined in the patient carrier of RCP. Conclusion: Our results suggest that there is ICE in the male carriers with a structural chromosomal abnormality, which appears to be translocation, breakpoint, chromosome, and patient dependent.en_US
dc.description.sponsorshipScientific Research Projects Coordination Unit at Selcuk University [:09102001]en_US
dc.description.sponsorshipProf Dr. Sait Bodur and Prof Dr. Tahsin Murad Aktan contributed to statistical analysis and semen analysis of the study. All authors declare that they have no conflict of interest in relation to this work. This work was supported by Scientific Research Projects Coordination Unit at Selcuk University (Grant number:09102001).en_US
dc.identifier.doi10.5152/tud.2020.19255
dc.identifier.endpage185en_US
dc.identifier.issn2149-3235
dc.identifier.issn2149-3057
dc.identifier.issue3en_US
dc.identifier.pmid32301691en_US
dc.identifier.scopus2-s2.0-85085024308en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage178en_US
dc.identifier.urihttps://doi.org/10.5152/tud.2020.19255
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16786
dc.identifier.volume46en_US
dc.identifier.wosWOS:000531866000003en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherAvesen_US
dc.relation.ispartofTurkish Journal Of Urologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAneuploidyen_US
dc.subjectChromosome Segregationen_US
dc.subjectGenetic Translocationen_US
dc.subjectFluorescent In Situ Hybridizationen_US
dc.subjectSpermatozoaen_US
dc.titleInvestigation of the interchromosomal effects in male carriers with structural chromosomal abnormalities using FISHen_US
dc.typeArticleen_US

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