The regulatory effects of biochar on PSII photochemistry, antioxidant system and nitrogen assimilation in Lemna minor exposed to inorganic pollutants, arsenic and fluoride

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dc.contributor.authorYildiztugay, Evren
dc.contributor.authorOzfidan-konakci, Ceyda
dc.contributor.authorArikan, Busra
dc.contributor.authorAlp-Turgut, Fatma Nur
dc.contributor.authorGuenturk, Cagri
dc.date.accessioned2024-02-23T14:12:46Z
dc.date.available2024-02-23T14:12:46Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractThe excessive accumulation of inorganic pollutants such as arsenic (As) and fluoride (F) are destructive contaminants for plants. There are some reports about the toxicity mechanisms of individual exposure of As or F. Biochar (BC), carbon-rich organic material, can sequester pollutants and help in increasing growth, nutrient uptake and water holding capacity in soils. However, the defense responses of plants to a combination of two stresses and the interactions between BC and combined exposure of As and F are not explicit. In order to fill this gap, different BC concentrations (BC1; 1 g L- 1 and BC2; 5 g L- 1) were hydroponically applied to Lemna minor subjected to sodium fluoride (F, 15 mM NaF) and sodium arsenate (As, 100 & mu;M Na2HAsO4). As or F pollutants caused a reduction in the chlorophyll fluorescence (Fv/Fm and Fv/Fo) but BC application under stress conditions improved the fluorescence levels. The photochemical reactions of photosystems and fluorescence kinetics impaired by stress were re-regulated through all BC concentrations. The relative expressions of genes belonging to proteins in PSI-PSII (psaA, psaB, psbA and psbD) were up-regulated in both BCs and As/F treatments. As-treated L. minor had only induction in superoxide dismutase-SOD- and ascorbate peroxidase-APX-, the enhancement in SOD, catalase-CAT-, glutathione reductase-GR- and glutathione S-transferase-GST- was observed under F toxicity. Also, the joint stress (As+F) caused declines in all antioxidant capacities except with SOD activity. Due to the sufficient antioxidant activation, hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances (TBARS) increased in L. minor. While, 1 mg L-1 BC induced the regeneration of ascorbate (AsA) under individual application of As or F, 5 mg L-1 BC increased APX, MDHAR, DHAR, total ascorbate (tAsA), and DHA against F stress. As- and NaF-mediated stress suppressed the nitrogen assimilation of L. minor. Interestingly, the induced accumulation of NH4+ was prevented in BC plus stress-applied plants through glutamate dehydrogenase (GDH) activity rather than glutamine synthetase-glutamate synthase (GS-GOGAT) pathways. All findings indicated that BCs reduced lipid peroxidation and ROS accumulation by modulating electron fluxes and photochemistry in PSI and PSII, gene expression, nitrogen metabolism as well as the antioxidant system under stress conditions (As, F, and As+F).en_US
dc.description.sponsorshipNecmettin Erbakan University Scientific Research Projects Coordinating Office; [221215001]en_US
dc.description.sponsorshipThis work was supported by Necmettin Erbakan University Scientific Research Projects Coordinating Office (Grant Number: 221215001) .en_US
dc.identifier.doi10.1016/j.jece.2023.110713
dc.identifier.issn2213-2929
dc.identifier.issn2213-3437
dc.identifier.issue5en_US
dc.identifier.scopus2-s2.0-85169805992en_US
dc.identifier.urihttps://doi.org/10.1016/j.jece.2023.110713
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12173
dc.identifier.volume11en_US
dc.identifier.wosWOS:001054593800001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevier Sci Ltden_US
dc.relation.ispartofJournal Of Environmental Chemical Engineeringen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectArsenicen_US
dc.subjectBiocharen_US
dc.subjectFluorideen_US
dc.subjectNitrogen Metabolismen_US
dc.subjectPsii Photochemistryen_US
dc.titleThe regulatory effects of biochar on PSII photochemistry, antioxidant system and nitrogen assimilation in Lemna minor exposed to inorganic pollutants, arsenic and fluorideen_US
dc.typeArticleen_US

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