The role of calcium entry on the relaxation response of rho-kinase inhibitor in rabbit renal artery

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dc.contributor.authorSoner, B. C.
dc.contributor.authorMurat, N.
dc.contributor.authorGuven, H.
dc.contributor.authorGidener, S.
dc.date.accessioned2024-02-23T14:37:57Z
dc.date.available2024-02-23T14:37:57Z
dc.date.issued2013
dc.departmentNEÜen_US
dc.description.abstractThis study was performed to clarify the role of extracellular and intracellular Ca2+ on rho-kinase enzyme inhibition-induced relaxation in rabbit renal arteries. The response to rho-kinase inhibitor (Y-27632) was studied in isolated renal artery segments precontracted with phenylephrine in the presence of voltage-gated calcium channel blocker nifedipine and in the absence of intracellular or extracellular Ca2+. Cumulative addition of rho-kinase inhibitor Y-27632 (10(-8)-10(-5) M) produced a concentration-dependent relaxation in renal artery rings precontracted with phenylephrine. Preincubation with nifedipine (1 mu M) resulted in a significant increase in relaxation response to rho-kinase inhibitor Y-27632 compared with preincubation with DMSO; the solvent of nifedipine. The maximal relaxation to Y-27632 in renal arteries precontracted with phenylephrine was significantly increased in the Ca-free Krebs containing 100 mu mol/l ethylene glycol tetraacetic acid (EGTA) but after depletion of intracellular stores with 20 mmol/l caffeine and 1mmol/l EGTA in Ca2+ free Krebs there was no significant difference between the relaxation to Y-27632 from control response in 2.5 mmol/l Ca2+ Krebs in the renal artery. These results suggest the involvement of extracellular Ca and L-type voltage-operated Ca2+ channels in phenylephrine-induced rho-kinase activation (Fig. 3, Ref. 20). Full Text in PDF www.elis.sk.en_US
dc.identifier.doi10.4149/BLL_2013_053
dc.identifier.endpage261en_US
dc.identifier.issn0006-9248
dc.identifier.issn1336-0345
dc.identifier.issue5en_US
dc.identifier.pmid23611047en_US
dc.identifier.scopus2-s2.0-84878914483en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage258en_US
dc.identifier.urihttps://doi.org/10.4149/BLL_2013_053
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16307
dc.identifier.volume114en_US
dc.identifier.wosWOS:000319719200003en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherComenius Univen_US
dc.relation.ispartofBratislava Medical Journal-Bratislavske Lekarske Listyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectRho-Kinaseen_US
dc.subjectCalciumen_US
dc.subjectRabbiten_US
dc.subjectRenal Arteryen_US
dc.titleThe role of calcium entry on the relaxation response of rho-kinase inhibitor in rabbit renal arteryen_US
dc.typeArticleen_US

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