Comparison of ELISA and RIA methods to quantify arginine vasopressin hormone levels in cell culture

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dc.contributor.authorTurkmen, Merve Ozcan
dc.contributor.authorKaraduman, Tugce
dc.contributor.authorMergen, Hatice
dc.date.accessioned2024-02-23T13:59:42Z
dc.date.available2024-02-23T13:59:42Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractThe radioimmunoassay (RIA) method is widely used to determine the levels of arginine vasopressin (AVP) in studies, especially in cell culture studies. However, there are many difficulties and disadvantages in performing this method. Therefore, this study aimed to assess the comparison between enzyme-linked immunosorbent assay (ELISA) and RIA methods by using the Bland-Altman statistical method and to investigate whether the commonly used RIA can be replaced by the ELISA method for measurement of AVP levels in cell culture medium. For this purpose, wild-type (WT) and three different mutant AVP-NPII vectors were transiently transfected to mouse neuroblastoma (Neuro2A) cells and AVP secretion into the cell culture medium by transfected Neuro2A cells was determined by both RIA and ELISA methods. Following the use of the normalization method, Bland-Altman method, currently the most commonly used statistical method assessing comparison between two methods, was performed to assess agreement between two methods. The order of normalized AVP values obtained using the RIA method was as follows: WT (0.921 +/- 0.08), 207_209delGGC mutant (0.801 +/- 0.09), G45C mutant (0.508 +/- 0.10), and G88V mutant (0.497 +/- 0.12). The normalized AVP values obtained using the ELISA method were as follows: WT (0.865 +/- 0.12), 207_209delGGC mutant (0.704 +/- 0.13), G88V mutant (0.255 +/- 0.16), and G45C mutant (0.250 +/- 0.15). The order of AVP levels measured from each transfected cell using both methods was quite similar. According to the Bland-Altman method, there is an agreement between RIA and ELISA for measuring the AVP levels in cell culture. It can be recommended to apply ELISA instead of the RIA method for determinating AVP levels.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK); [115S499]en_US
dc.description.sponsorshipAcknowledgementsWe gratefully thank Dr. Muhittin SERDAR and his team for providing the gamma counter in their laboratory for the radioimmunoassay. This study is a part of the Ph. D. thesis of the first author and financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK, Project number: 115S499).en_US
dc.identifier.doi10.1007/s11756-022-01301-6
dc.identifier.endpage894en_US
dc.identifier.issn0006-3088
dc.identifier.issn1336-9563
dc.identifier.issue3en_US
dc.identifier.scopus2-s2.0-85145060546en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage887en_US
dc.identifier.urihttps://doi.org/10.1007/s11756-022-01301-6
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11281
dc.identifier.volume78en_US
dc.identifier.wosWOS:000905474100001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofBiologiaen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAvpen_US
dc.subjectVasopressinen_US
dc.subjectElisaen_US
dc.subjectRiaen_US
dc.subjectBland-Altmanen_US
dc.titleComparison of ELISA and RIA methods to quantify arginine vasopressin hormone levels in cell cultureen_US
dc.typeArticleen_US

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