Subtype distribution and molecular characterization of Blastocystis from hemodialysis patients in Turkey

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dc.contributor.authorGulhan, Baris
dc.contributor.authorAydin, Merve
dc.contributor.authorDemirkazik, Mehtap
dc.contributor.authorKoltas, Ismail Soner
dc.contributor.authorCikman, Aytekin
dc.contributor.authorTurkmen, Kultigin
dc.contributor.authorDuran, Tugce
dc.date.accessioned2024-02-23T14:35:25Z
dc.date.available2024-02-23T14:35:25Z
dc.date.issued2020
dc.departmentNEÜen_US
dc.description.abstractIntroduction: The aim of this study was to determine the Blastocystis prevalence and subtypes in hemodialysis patients in Turkey. Methodology: Eighty-four patients diagnosed with end-stage renal failure who were undergoing hemodialysis and 20 healthy volunteers were enrolled. Blastocystis presence was investigated by native-Lugol, trichrome staining, PCR using STS primers, and DNA sequencing analysis. Results: Among the stool samples from the hemodialysis patients, 9.52% (8/84) were found to be Blastocystis-positive with the native-Lugol and trichrome staining. Seven of the eight Blastocystis isolates were subtyped using STS primers. Blastocystis subtype distribution was as follows: one had ST1, two had ST2, two had ST3, two had ST3+ST6, and one was not subtyped. Blastocystis positivity was detected in two healthy control (2/20, %10), one subject had ST1, and the other was not subtyped. All subtypes identified by PCR were confirmed by the sequencing analysis. In the two samples that had mixed subtypes (ST3+ST6) when using the STS primers, only ST3 was detected in the sequencing analysis. Although some patients have multiple symptoms, the most common symptoms in Blastocystis positive patients were bloating (5/8), diarrhea (4/8), nausea and vomiting (2/8), and gas and weight loss (1/8). Also, only one patient had Giardia intestinalis. Conclusions: This was the first study to determine the Blastocystis subtypes in hemodialysis patients. A rare subtype, ST6, was identified in two of the patients. Thus, the ST6 infections were attributable to transmission from poultry infections. The presence of this unusual subtype suggests the need for further extensive studies of hemodialysis patients.en_US
dc.identifier.doi10.3855/jidc.12650
dc.identifier.endpage1454en_US
dc.identifier.issn1972-2680
dc.identifier.issue12en_US
dc.identifier.pmid33378289en_US
dc.identifier.scopus2-s2.0-85099082672en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage1448en_US
dc.identifier.urihttps://doi.org/10.3855/jidc.12650
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16024
dc.identifier.volume14en_US
dc.identifier.wosWOS:000605192900015en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherJ Infection Developing Countriesen_US
dc.relation.ispartofJournal Of Infection In Developing Countriesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBlastocystisen_US
dc.subjectHemodialysis Patientsen_US
dc.subjectMolecular Characterizationen_US
dc.subjectSt6en_US
dc.subjectSubtypeen_US
dc.titleSubtype distribution and molecular characterization of Blastocystis from hemodialysis patients in Turkeyen_US
dc.typeArticleen_US

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