Immunoaffinity microcryogels for purification of transferrin

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dc.contributor.authorCetin, Kemal
dc.contributor.authorDenizli, Adil
dc.date.accessioned2024-02-23T14:12:34Z
dc.date.available2024-02-23T14:12:34Z
dc.date.issued2019
dc.departmentNEÜen_US
dc.description.abstractImmunoaffinity chromatography has a huge interest in the biomedical and biotechnological fields, in particular for one-step isolation, purification and removal of analyte compounds. In this study, uniform-sized microcryogels, a new type of cryogels, were synthesized using 2-hydroxyetyhl methacrylate and epoxy-group-containing monomer, glycidyl methacrylate for purification of a plasma protein, transferrin. Immunoaffinity microcryogels containing anti-Tf antibodies were characterized by Raman spectroscopy, Fourier transform infrared spectroscopy, optical microscopy, scanning electron microscopy, density measurements and swelling tests. Adsorption studies in aqueous media were carried out in order to examine the effects of medium pH, initial concentration of analyte and contact time. It was found that the optimum pH was 6.0 and the maximum adsorption capacity of immunoaffinity microcryogels at this pH value found to be 9.82 mg/g. The K-L constant for Langmuir isotherm was calculated as 2.65 mL/mg. The maximum adsorption capacity obtained from experimental studies is also very close to the calculated Langmuir adsorption capacity (11.27 mg/g). Langmuir adsorption isotherms and pseudo-second-order kinetic models are consistent with the adsorption process, which means that the adsorption is single layered and chemically controlled. The purity of the eluted hsTf from plasma was about 84% with yield about 82%. After the tenth use of the same microcryogels, the maximum hsTf adsorption capacity decreased by about 20%. The results indicated that the immunoaffinity microcryogels having anti-Tf antibody ligands could be a safe and cost-friendly method for purification of transferrin.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK)en_US
dc.description.sponsorshipKemal Cetin thanks The Scientific and Technological Research Council of Turkey (TUBITAK) for the support by TUBITAK-BIDEB 2211-National Ph.D. Scholarship Programme.en_US
dc.identifier.doi10.1016/j.jchromb.2019.03.017
dc.identifier.endpage12en_US
dc.identifier.issn1570-0232
dc.identifier.issn1873-376X
dc.identifier.pmid30909060en_US
dc.identifier.scopus2-s2.0-85063192575en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage5en_US
dc.identifier.urihttps://doi.org/10.1016/j.jchromb.2019.03.017
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12115
dc.identifier.volume1114en_US
dc.identifier.wosWOS:000465192800002en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Science Bven_US
dc.relation.ispartofJournal Of Chromatography B-Analytical Technologies In The Biomedical And Life Sciencesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAnti-Transferrinen_US
dc.subjectImmunoaffinity Chromatographyen_US
dc.subjectMicrocryogelsen_US
dc.subjectProtein Purificationen_US
dc.subjectTransferrinen_US
dc.titleImmunoaffinity microcryogels for purification of transferrinen_US
dc.typeArticleen_US

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