Efficacy of Light Emitting diodes (LEDs) Lighting System for In vitro Shoot Regeneration of Medicinal Water hyssop (Bacopa monnieri L. PENNEL)

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dc.contributor.authorKaratas, Mehmet
dc.contributor.authorAasim, Muhammad
dc.contributor.authorDazkirili, Murat
dc.date.accessioned2024-02-23T14:49:07Z
dc.date.available2024-02-23T14:49:07Z
dc.date.issued2018
dc.departmentNEÜen_US
dc.description.abstractEfficacy of different Light Emitting Diodes (LEDs) on in vitro whole plant regeneration of water hyssop (Bacopa monnieri L. Pennel) was investigated in this study. Shoot tip explants were cultured on MS medium with BA (0.25, 0.50 and 1.0 mg l(-1)) and incubated under Red: Blue (R:B) LEDs in different combinations (4:1, 3:1, 2:1 and 1:1 R: B and white LEDs) for 10 weeks. Callus induction and shoot regeneration was noted on all explants irrespective of LEDs type or BA concentration. Shoots per explant and shoot length ranged 6.56-10.42 and 0.96-1.71cm respectively. Maximum number of 10.42 shoots with highest shoot length of 1.71 cm was achieved under 1:1 R:B LEDs. Whereas, different BA concentrations generated 4.97-11.98 shoots per explant with shoot length in the range of 0.93-1.35 cm. Maximum shoots per explant (10.35) with more longer shoots (1.31 cm) was achieved on MS medium containing 1.0 mg l(-1) BA. Comparing of BA x LEDs, maximum number of 15.17 shoots per explant was induced under 1.0 mg l(-1) BA x 2:1 R: B LEDs. Whereas, longer shoots (2.68 cm) were scored under 0.5 mg l(-1) BA x 2:1 R: B LEDs. Regenerated shoots were rooted using 1.0 mg l(-1) IBA in four weeks followed by acclimatization in the aquariums provided with tap water. Results revealed that LEDs in combination with BA can be successfully employed for multiple shoot regeneration of water hyssop.en_US
dc.identifier.endpage13204en_US
dc.identifier.issn1224-5984
dc.identifier.issue1en_US
dc.identifier.startpage13197en_US
dc.identifier.urihttps://hdl.handle.net/20.500.12452/18008
dc.identifier.volume23en_US
dc.identifier.wosWOS:000429181000001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.language.isoenen_US
dc.publisherArs Docendien_US
dc.relation.ispartofRomanian Biotechnological Lettersen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectIn Vitroen_US
dc.subjectLight Emitting Diodesen_US
dc.subjectShoot Regenerationen_US
dc.subjectWater Hyssopen_US
dc.titleEfficacy of Light Emitting diodes (LEDs) Lighting System for In vitro Shoot Regeneration of Medicinal Water hyssop (Bacopa monnieri L. PENNEL)en_US
dc.typeArticleen_US

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