Immunohistochemical Evaluation of TNF-?, IL-1, IL-12, IL-17, IL-23 Expression and Investigation of the Effect of Demodex in Patients with Discoid Lupus Erythematosus

Basit Öğe Kaydı
dc.contributor.authorDursun, Recep
dc.contributor.authorTemiz, Selami Aykut
dc.contributor.authorFindik, Siddika
dc.contributor.authorDurmaz, Koray
dc.contributor.authorOltulu, Pembe
dc.date.accessioned2024-02-23T14:38:20Z
dc.date.available2024-02-23T14:38:20Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractObjective: Discoid lupus erythematosus (DLE) is a chronic inflammatory skin disease that can be triggered by several factors although its etiology is not yet known. Hypotheses have been reported that the demodex mites may be involved in the etiopathogenesis of DLE. In this study, we aimed to investigate the potential relationship between the frequency of immunohistochemical staining of tumor necrosis factor (TNF)-a, interleukin (IL)-1, IL-12, IL-17 and IL-23 cytokines obtained from cutaneous biopsy of DLE patients and disease severity. Materials and Methods: Biopsy tissues of patients who were previously diagnosed with DLE in the dermatology outpatient clinic, which were also supported histopathologically, were re-sectioned and subjected to immunohistochemical examination for TNF-a, IL-1, IL-12, IL-17 and IL-23, and their staining scores were obtained. These immunohistochemical staining scores were compared with disease severity. The presence and density of demodex were evaluated in standard skin surface biopsy taken from the lesions at the time of diagnosis. Results: In the comparison of immunohistochemical staining scores with DLE-skin score (DLE-SS), a statistically significant positive correlation was found between DLE-SS and TNF-a (p=0.003), DLE-SS and IL-17 (p=0.002). There was no difference between the presence or absence of demodex and DLE-SS (p=0.9). There was no correlation between demodex density and disease severity in demodex-positive cases (p=0.34). Conclusion: In line with the data obtained from our study, TNF-a and IL-17 seem to be more associated with the disease severity in DLE. The fact that demodex positivity/negativity and demodex density are independent of disease severity supports that demodex mite is an etiopathogenetic factor rather than overlap in DLE cases. Further studies on this subject are needed.en_US
dc.identifier.doi10.4274/tji.galenos.2023.63626
dc.identifier.endpage28en_US
dc.identifier.issn1301-109X
dc.identifier.issn2147-8325
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85159365497en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage23en_US
dc.identifier.urihttps://doi.org/10.4274/tji.galenos.2023.63626
dc.identifier.urihttps://hdl.handle.net/20.500.12452/16453
dc.identifier.volume11en_US
dc.identifier.wosWOS:000988120700004en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherGalenos Publ Houseen_US
dc.relation.ispartofTurkish Journal Of Immunologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDiscoid Lupus Erythematosusen_US
dc.subjectImmunohistochemistryen_US
dc.subjectTnf-Alphaen_US
dc.subjectIl-1en_US
dc.subjectIl-12en_US
dc.subjectIl-17en_US
dc.subjectIl-23en_US
dc.titleImmunohistochemical Evaluation of TNF-?, IL-1, IL-12, IL-17, IL-23 Expression and Investigation of the Effect of Demodex in Patients with Discoid Lupus Erythematosusen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu