Evaluation of Apoptosis Pathway of Geraniol on Ishikawa Cells

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dc.contributor.authorKuzu, Betul
dc.contributor.authorCuce, Gokhan
dc.contributor.authorAyan, Ilknur Cinar
dc.contributor.authorGultekin, Burcu
dc.contributor.authorCanbaz, Halime Tuba
dc.contributor.authorDursun, Hatice Gul
dc.contributor.authorSahin, Zafer
dc.date.accessioned2024-02-23T14:17:14Z
dc.date.available2024-02-23T14:17:14Z
dc.date.issued2021
dc.departmentNEÜen_US
dc.description.abstractEndometrial cancer is the most common type of cancer in the female reproductive system. Geraniol is acyclic monoterpene alcohol derived from essential oils of aromatic plants. This study aimed to investigate the apoptosis pathway of geraniol on Ishikawa cells. The cytotoxic effects of Geraniol on Ishikawa cells were determined by an MTT test. Ishikawa cells were seeded on cover slips, the IC50 dose was applied, and the cells were incubated with antibodies against Bax, Bcl-2, and TUNEL Assay. mRNA expression analysis of apoptosis-related genes was determined by RT-qPCR with an IC50 dose of Geraniol. The IC50 dose of Geraniol decreased Bcl-2 staining significantly, but it significantly increased Bax staining and TUNEL positive cells. A significant increase in the Bax, caspase3, caspase-8, cytochrome C and Fas genes and a significant decrease in the Bcl-2 gene was observed when the IC50 dose group was compared to the cells in the control group based on their mRNA expression levels.Analysis of expression of genes whose products are involved in apoptosis suggests the involvement of the mitochondrial pathway.en_US
dc.description.sponsorshipScientific Research Projects Coordination Office of Necmettin Erbakan University, Konya, Turkey [191318001]en_US
dc.description.sponsorshipOur research was supported by a grant from the Scientific Research Projects Coordination Office of Necmettin Erbakan University, Konya, Turkey, Project No. 191318001.en_US
dc.identifier.doi10.1080/01635581.2020.1836244
dc.identifier.endpage2537en_US
dc.identifier.issn0163-5581
dc.identifier.issn1532-7914
dc.identifier.issue11.Araen_US
dc.identifier.pmid33086902en_US
dc.identifier.scopus2-s2.0-85093672483en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage2532en_US
dc.identifier.urihttps://doi.org/10.1080/01635581.2020.1836244
dc.identifier.urihttps://hdl.handle.net/20.500.12452/13010
dc.identifier.volume73en_US
dc.identifier.wosWOS:000582354300001en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherRoutledge Journals, Taylor & Francis Ltden_US
dc.relation.ispartofNutrition And Cancer-An International Journalen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subject[Keyword Not Available]en_US
dc.titleEvaluation of Apoptosis Pathway of Geraniol on Ishikawa Cellsen_US
dc.typeArticleen_US

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