Çinko oksit nanopartikülleri ve siklofosfamidin sıçanlarda testis histolojisi,apoptozis ve oksidan-antioksidan değerler üzerine etkisi
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Dosyalar
Tarih
2016
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Necmettin Erbakan Üniversitesi Sağlık Bilimleri Enstitüsü
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Siklofosfamid (CP) çocukluk ve yetişkin malignensilerde, organ nakillerinde immun supresif olarak, sistemik lupus eritematoz, multiple skleroz gibi hastalıklarda kullanılan kemoterapötik bir ilaçtır. Çinko oksit (ZnO) çok amaçlı inorganik bir malzeme olup ZnO nanopartikülleri ise boya, pigment, kozmetik ve kişisel bakım gibi farklı endütriyel alanlarda kullanılan en yaygın malzemelerden birisidir. Bu çalışmada testislerde hasar oluşturduğu bilinen CP ile testis hasarı ve apoptozis çalışmaların yetersiz kaldığı ZnO nanopartikül uygulamasının etkileri amaçlanmıştır. Sıçanlar; I. Grup (n=7) Kontrol, II. Grup (n=7) Siklofosfamid (20 mg/kg/gün ip) (CP), III. Grup (n=7) Çinko oksit (ZnO) nanopartikül (300 mg/kg/gün oral) ve IV. Grup (n=7) CP (20 mg/kg/gün ip) + ZnO (300 mg/kg/gün oral) olmak üzere 4 gruba ayrıldı. Tüm gruplara, 7 gün boyunca maddeler enjeksiyon ve oral yolla verildi. Sıçanların sol testislerine Hematoksilen-Eozin, TUNEL, Bax ve Caspase-3 immünohistokimyasal teknikleri uygulandı. Sağ testis doku homojenatları ile kan serumları ise oxidative stress markers (indirgenmiş Glutatyon, Catalase, TBARS) ölçüldü. Gruplar; Johnsen skorlama bakımından karşılaştırıldığında, tüm gruplar arasında anlamlı bir fark bulundu ve en düşük skor Grup 4'de gözlendi. İmmünohistokimyasal Bax boyanmasında Grup 2 ve Grup 3 arasında anlamlı bir fark gözlemlenmedi, bunun dışındaki diğer bütün gruplar arasında anlamlı fark ortaya çıkmıştır. Caspase-3 boyanmasında ise Grup 1 – Grup 2, Grup 1 – Grup 4, Grup 2 – Grup 4 ve Grup 3 – Grup 4 arasında istatistiksel olarak anlamlı fark çıkmıştır. TUNEL metodu sonucuna göre ise bütün gruplar arasında anlamlı bir fark olduğu görülmüştür. Serum GSH ve Katalaz seviyesi, sadece Grup 2 ve Grup 4'de benzerlik göstermiştir ve diğer gruplarda birbirinden anlamlı derecede farklı çıkmıştır. Serum TBARS seviyesi ise bütün gruplar arasında anlamlı bir farklılık göstermiştir. Doku GSH seviyesi, Grup1 – Grup 3 ile Grup 2 – Grup 4 arasında benzer olup diğer gruplarda farklılık göstermiştir. Doku Katalaz seviyesi, Grup 2 – Grup 3 gruplarında benzer olup diğer gruplarda anlamlı farklılık göstermiştir. Doku TBARS seviyesi ise bütün gruplarda anlamlı farklılık göstermiştir. Sonuç olarak, çinko oksitin testislere, siklofosfamidden daha az zarar verdiği tespit edildi. Testislerde en fazla hasarın, ikisinin birlikte kullanıldığı grupta oluştuğu gözlendi.
Cyclophosphamide (CP) is a chemotherapeutic drug used in diseases such as multiple sclerosis systemic lupus erythematosus, as immuno suppressive in organ transplantation in the childhood and adult malignancies. Zinc oxide (ZnO) is a multi purpose inorganic material and ZnO nanoparticlesis are one of the most common used in various industrial fields such as paint, pigment, cosmetics and personal care. In this study the effects of CP which is known cause damage to the testicles and the effects of ZnO nanoparticles applications which studies are not sufficient for testicular damage and apoptosis were investigated. Rats were grouped into four as: I. Group (n=7) Control, II. Group (n=7) Cyclophosphamide (CP) (20 mg/kg/day ip), III. Group (n=7) Zinc oxide (ZnO) nanoparticles (300 mg/kg/day oral) and IV. Group (n = 7) CP + ZnO (20 mg/kg/day ip + 300 mg/kg/day oral). Elements were intraperitoneally injected and orally given to all groups for seven days. Hematoxilene-Eosine, TUNEL, Bax and Caspase-3 immunohistochemical techniques were applied to left testis. Right testis tissue homojenants and blood serums of oxidative stres markers (reduced glutation, catalase, TBARS)were measured. When Johnsen scoring terms compared, a significant difference was found between all groups and the lowest score was observed in the Group 4. In Bax immunohistochemical staining, a significant difference between Group 2 and Group 3 wasn't observed, all other groups had significant difference. In Caspase-3 staining, a statistically significant difference between Group 1 – Group 2, Group 1 – Group 4, Group 2 – Group 4 and Group 3 – Group 4 were observed. A significant difference between all groups was found when compared to control group. Serum levels of GSH and catalase were similar between Group 2 and Group 4 and were significantly different in all other groups. Serum TBARS levels showed a significant difference between all groups. Tissue GSH level was similar in Group 1 – Group 3 and Group 2 – Group 4, it showed significant differences in the other groups. Tissue levels of catalase, was similar in Group 2 – Group 3, significant differences were found in the other groups. Tissue TBARS levels showed significant differences in all groups. In conclusion, it was observed that zinc oxide has given less damage to the testicles than cyclophosphamide. Maximum damage was observed in combined group in the testes.
Cyclophosphamide (CP) is a chemotherapeutic drug used in diseases such as multiple sclerosis systemic lupus erythematosus, as immuno suppressive in organ transplantation in the childhood and adult malignancies. Zinc oxide (ZnO) is a multi purpose inorganic material and ZnO nanoparticlesis are one of the most common used in various industrial fields such as paint, pigment, cosmetics and personal care. In this study the effects of CP which is known cause damage to the testicles and the effects of ZnO nanoparticles applications which studies are not sufficient for testicular damage and apoptosis were investigated. Rats were grouped into four as: I. Group (n=7) Control, II. Group (n=7) Cyclophosphamide (CP) (20 mg/kg/day ip), III. Group (n=7) Zinc oxide (ZnO) nanoparticles (300 mg/kg/day oral) and IV. Group (n = 7) CP + ZnO (20 mg/kg/day ip + 300 mg/kg/day oral). Elements were intraperitoneally injected and orally given to all groups for seven days. Hematoxilene-Eosine, TUNEL, Bax and Caspase-3 immunohistochemical techniques were applied to left testis. Right testis tissue homojenants and blood serums of oxidative stres markers (reduced glutation, catalase, TBARS)were measured. When Johnsen scoring terms compared, a significant difference was found between all groups and the lowest score was observed in the Group 4. In Bax immunohistochemical staining, a significant difference between Group 2 and Group 3 wasn't observed, all other groups had significant difference. In Caspase-3 staining, a statistically significant difference between Group 1 – Group 2, Group 1 – Group 4, Group 2 – Group 4 and Group 3 – Group 4 were observed. A significant difference between all groups was found when compared to control group. Serum levels of GSH and catalase were similar between Group 2 and Group 4 and were significantly different in all other groups. Serum TBARS levels showed a significant difference between all groups. Tissue GSH level was similar in Group 1 – Group 3 and Group 2 – Group 4, it showed significant differences in the other groups. Tissue levels of catalase, was similar in Group 2 – Group 3, significant differences were found in the other groups. Tissue TBARS levels showed significant differences in all groups. In conclusion, it was observed that zinc oxide has given less damage to the testicles than cyclophosphamide. Maximum damage was observed in combined group in the testes.
Açıklama
Yüksek Lisans Tezi
Anahtar Kelimeler
Apoptozis, Çinko oksit nanopartikülü, Siklofosfamid, Testis, Apoptosis, zinc oxide nanoparticles, cyclophosphamide, testicular
Kaynak
WoS Q Değeri
Scopus Q Değeri
Cilt
Sayı
Künye
Atasoy, N. (2016). Çinko oksit nanopartikülleri ve siklofosfamidin sıçanlarda testis histolojisi,apoptozis ve oksidan-antioksidan değerler üzerine etkisi. (Yayınlanmamış Yüksek Lisans Tezi). Necmettin Erbakan Üniversitesi, Sağlık Bilimleri Enstitüsü Histoloji ve Embriyoloji Anabilim Dalı, Konya.
