Polyamine Cadaverine Detoxifies Nitrate Toxicity on the Chloroplasts of Triticum aestivum Through Improved Gas Exchange, Chlorophyll a Fluorescence and Antioxidant Capacity

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dc.contributor.authorBalci, Melike
dc.contributor.authorAlp, Fatma Nur
dc.contributor.authorArikan, Busra
dc.contributor.authorOzfidan-Konakci, Ceyda
dc.contributor.authorYildiztugay, Evren
dc.date.accessioned2024-02-23T13:43:43Z
dc.date.available2024-02-23T13:43:43Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractNitrate (NO3-) toxicity is a serious problem that threatens the health of living organisms and especially agricultural production. The presence of NO3- leads to biomass loss by causing the imbalance of biochemical metabolism and inhibiting photosynthetic activity. A new critical approach to cope with nitrate toxicity is the use of polyamines (PAs) as an antioxidant defence system enhancer in plants. However, there is no information about the impacts of cadaverine, is one of PAs, on chloroplasts of plants exposed to NO3- toxicity. For this purpose, this study focused on the photosynthetic and biochemical process taking place in chloroplasts of Triticum aestivum under nitrate stress (100 mM and 200 mM NO3-) and/or cadaverine (100 mu M and 1 mM Cad). Gas exchange, chlorophyll fluorescence (F-v/F-m and F-v/F-o), the efficiency of the light reaction (phi P-o/(1 - phi P-o)), the performance index (PItotal), and relative water content (RWC) levels were suppressed under NO3- stress. Stress did not improve the antioxidant activities in chloroplasts such as superoxide dismutase (SOD), glutathione reductase (GR). After 100 mM NO3- exposure, Cad increased chloroplastic SOD, peroxidase (POX), ascorbate peroxidase (APX), GR, monodehydroascorbate reductase (MDHAR), and glutathione S-transferase (GST) activities. In the presence of 200 mM NO3-, Cads decreased SOD and GST activity. In NO3- + Cad-applied wheat, the high contents of hydrogen peroxide (H2O2) and lipid peroxidation (TBARS) were effectively removed through ascorbate (AsA) regeneration. Cads promoted the maintenance of cellular redox state by regulating antioxidant pathways included in the ascorbate-glutathione (AsA-GSH) cycle. Our results showed that Cad has great potential to confer tolerance to wheat by reducing oxidative damage and protecting the biochemical reactions of photosynthesis against NO3- toxicity.en_US
dc.description.sponsorshipSelcuk University Scientific Research Projects Coordinating Office [20201038]en_US
dc.description.sponsorshipThis work was supported by Selcuk University Scientific Research Projects Coordinating Office (Grant Number: 20201038).en_US
dc.identifier.doi10.1007/s00344-022-10749-4
dc.identifier.endpage4974en_US
dc.identifier.issn0721-7595
dc.identifier.issn1435-8107
dc.identifier.issue8en_US
dc.identifier.scopus2-s2.0-85135833174en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage4958en_US
dc.identifier.urihttps://doi.org/10.1007/s00344-022-10749-4
dc.identifier.urihttps://hdl.handle.net/20.500.12452/10893
dc.identifier.volume42en_US
dc.identifier.wosWOS:000839537200001en_US
dc.identifier.wosqualityQ1en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofJournal Of Plant Growth Regulationen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAntioxidant Systemen_US
dc.subjectCadaverineen_US
dc.subjectNitrate Toxicityen_US
dc.subjectPhotosynthesisen_US
dc.subjectTriticum Aestivum Len_US
dc.titlePolyamine Cadaverine Detoxifies Nitrate Toxicity on the Chloroplasts of Triticum aestivum Through Improved Gas Exchange, Chlorophyll a Fluorescence and Antioxidant Capacityen_US
dc.typeArticleen_US

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