Investigation of Children with Acute Gastroenteritis by Multiplex PCR Method in Central Part of Turkey

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dc.contributor.authorYilmaz, Fatih
dc.contributor.authorKaya, Havva
dc.contributor.authorOzdemir, Mehmet
dc.date.accessioned2024-02-23T14:16:59Z
dc.date.available2024-02-23T14:16:59Z
dc.date.issued2022
dc.departmentNEÜen_US
dc.description.abstractObjective Gastroenteritis is a disease that affects all age groups, especially children, and causes high mortality and morbidity in all countries. The most common agents of acute gastroenteritis are viral agents. As a result, millions of diarrhea attacks and hospital admissions occur worldwide every year due to viral gastroenteritis. This study uses the multiplex polymerase chain reaction (PCR) method to investigate the viruses that are the causative agents of viral gastroenteritis in the pediatric patient group in Konya, Turkey. Methods Stool samples of 94 patients aged 0 to 18 years sent from Emergency clinics and Pediatric outpatient clinics, Meram Medical Faculty Hospital Pediatric clinics, Konya Necmettin Erbakan University to Medical Microbiology Laboratory with a diagnosis of gastroenteritis between February and December 2018 were included in the study. Stool samples were stored at -80 degrees C until the time of the analysis. Deoxyribonucleic acid/ribonucleic acid isolation from stool samples was performed with EZ1 Virus Mini Kit v2.0 (Qiagen, Hilden, Germany) using an automatic extraction system (BioRobot EZ1 system, Qiagen). The presence of astrovirus, rotavirus, adenovirus, norovirus (GI, GII), and sapovirus agents was investigated by the multiplex PCR method (Fast Track Diagnostics, Luxembourg) viral gastroenteritis kit. Results Viral gastroenteritis agents were detected in 56.3% of the patients. One viral agent was detected in 47 (50%) of these patients and at least two viral agents in 6 (6.3%) of them. Norovirus GII was detected in 20 (21.2%) of the children included in the study, adenovirus in 13 (13.8%), rotavirus in 11 (12.8%), astrovirus in 11 (11.7%), sapovirus in 4 (4.2%), and norovirus GI in 1 (1.06%). When the distribution of viral agents was examined by months, the most number of agents were observed (21; 35%) in May, followed by April and June (12; 20%). Considering the distribution of the prevalence of the agents by age, it was seen to be mainly between 0 and 12 months (42%). Conclusion Considering that the most common viral agent in our region is norovirus GII, it will be useful to investigate the norovirus that is not routinely examined in children who are admitted to clinics with the complaint of gastroenteritis. It will be appropriate to examine routinely adenovirus, rotavirus, and norovirus in the laboratory, especially in children with diarrhea and vomiting in the winter and spring months.en_US
dc.identifier.doi10.1055/s-0041-1740372
dc.identifier.endpage52en_US
dc.identifier.issn1305-7707
dc.identifier.issn1305-7693
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85123002140en_US
dc.identifier.scopusqualityQ3en_US
dc.identifier.startpage48en_US
dc.identifier.urihttps://doi.org/10.1055/s-0041-1740372
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12878
dc.identifier.volume17en_US
dc.identifier.wosWOS:000739633900001en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherGeorg Thieme Verlag Kgen_US
dc.relation.ispartofJournal Of Pediatric Infectious Diseasesen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGastroenteritisen_US
dc.subjectMultiplex Pcren_US
dc.subjectRotavirusen_US
dc.subjectAdenovirusen_US
dc.subjectNorovirusen_US
dc.subjectSapovirusen_US
dc.subjectAstrovirusen_US
dc.titleInvestigation of Children with Acute Gastroenteritis by Multiplex PCR Method in Central Part of Turkeyen_US
dc.typeArticleen_US

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