Mammalian Sex Hormones as Steroid-Structured Compounds in Wheat Seedling: Template of the Cytosine Methylation Alteration and Retrotransposon Polymorphisms with iPBS and CRED-iBPS Techniques

Basit Öğe Kaydı
dc.contributor.authorDemirel, Fatih
dc.contributor.authorTurkoglu, Aras
dc.contributor.authorHaliloglu, Kamil
dc.contributor.authorEren, Baris
dc.contributor.authorOzkan, Guller
dc.contributor.authorUysal, Pinar
dc.contributor.authorPour-Aboughadareh, Alireza
dc.date.accessioned2024-02-23T14:35:05Z
dc.date.available2024-02-23T14:35:05Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractPhytohormones are chemical compounds found naturally in plants that have a significant effect on their growth and development. The increase in research on the occurrence of mammalian sex hormones (MSHs) in plants has prompted the need to investigate the functions performed by these hormones in plant biology. In the present study, we investigated the effects of MSHs on DNA damage and DNA methylation of wheat (Triticum aestivum L.) during the seedling growth stage, using the CRED-iPBS (coupled restriction enzyme digestion/inter primer binding site) assay and iPBS analysis to determine DNA methylation status. Exogenous treatment with four MSHs (17-& beta;-estradiol, estrogen, progesterone, and testosterone) was carried out at four different concentrations (0, 0.05, 0.5, and 5 & mu;M). The highest genomic template stability (GTS) value (80%) was observed for 5 & mu;M 17-& beta;-estradiol, 0.5 & mu;M testosterone, and 0.05 & mu;M estrogen, while the lowest value (70.7%) was observed for 5 & mu;M progesterone and 0.5 & mu;M estrogen. The results of the CRED-iPBS analysis conducted on MspI indicate that the 0.05 & mu;M estrogen-treated group had the highest polymorphism value of 40%, while the 5 & mu;M progesterone-treated group had the lowest value of 20%. For HpaII, treatment with 0.5 & mu;M 17-& beta;-estradiol had the highest polymorphism value of 33.3%, while the group treated with 0.05 & mu;M 17-& beta;-estradiol and 0.05 & mu;M progesterone had the lowest value of 19.4%. In conclusion, MSH treatments altered the stability of the genomic template of wheat plants and affected the cytosine methylation status at the seedling growth stage. Upon comprehensive examination of the results, it was seen that the employed methodology successfully detected alterations in cytosine methylation of genomic DNA (gDNA), as well as changes in the pattern of genomic instability.en_US
dc.description.sponsorshipThe authors thank the anonymous reviewers and editors for their constructive comments on this manuscript.en_US
dc.description.sponsorshipThe authors thank the anonymous reviewers and editors for their constructive comments on this manuscript.en_US
dc.identifier.doi10.3390/app13179538
dc.identifier.issn2076-3417
dc.identifier.issue17en_US
dc.identifier.scopus2-s2.0-85170375992en_US
dc.identifier.urihttps://doi.org/10.3390/app13179538
dc.identifier.urihttps://hdl.handle.net/20.500.12452/15862
dc.identifier.volume13en_US
dc.identifier.wosWOS:001061866600001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherMdpien_US
dc.relation.ispartofApplied Sciences-Baselen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectWheaten_US
dc.subjectIpbsen_US
dc.subjectPolymorphismen_US
dc.subjectCytosine Methylationen_US
dc.subjectMshen_US
dc.titleMammalian Sex Hormones as Steroid-Structured Compounds in Wheat Seedling: Template of the Cytosine Methylation Alteration and Retrotransposon Polymorphisms with iPBS and CRED-iBPS Techniquesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu