In vitro propagation of the threatened plant Sphaerophysa kotschyana (Fabaceae): inter simple-sequence-repeat (ISSR) analysis and salt tolerance of the regenerants

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dc.contributor.authorErisen, Semiha
dc.contributor.authorOncel, Zeynep
dc.date.accessioned2024-02-23T14:17:06Z
dc.date.available2024-02-23T14:17:06Z
dc.date.issued2013
dc.departmentNEÜen_US
dc.description.abstractSphaerophysa kotschyana is a threatened endemic species in Turkey and according to the Bern Convention, it is on the absolute preservation plant list. In vitro propagation methodologies were evaluated as an ex situ conservation strategy for this species. Nodal segments were cultured on Murashige and Skoog (MS) media with different cytokinins (benzyladenine, thidiazuron (TDZ) and zeatine), with or without auxin (a-naphthaleneacetic acid; NAA), to investigate shoot initiation. TDZ produced the highest number of shoots (11.0 shoots per explant) on MS medium at a concentration of 0.05 mg L-1. Rooting reached 100% when 0.5 mg L-1 NAA was combined with half strength MS and 1.5% sucrose and rooted plantlets were successfully acclimatised. Somaclonal variation of a mother plant and 10 regenerants was assessed using ISSR analysis. The same banding profiles were exhibited by all plants. In vitro response to salinity stress (NaCl) was also investigated in this halophytic species. Higher concentrations of NaCl negatively affected shoot multiplication, whereas shoot height was enhanced at 50mM NaCl. These results suggest that the established protocol is an efficient and reliable system of in vitro propagation for ex situ conservation of S. kotschyana.en_US
dc.identifier.doi10.1071/BT12163
dc.identifier.endpage72en_US
dc.identifier.issn0067-1924
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-84874283344en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage67en_US
dc.identifier.urihttps://doi.org/10.1071/BT12163
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12946
dc.identifier.volume61en_US
dc.identifier.wosWOS:000315153600008en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherCsiro Publishingen_US
dc.relation.ispartofAustralian Journal Of Botanyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectEndemicen_US
dc.subjectHalophyteen_US
dc.subjectMicropropagationen_US
dc.subjectSomaclonal Variationen_US
dc.titleIn vitro propagation of the threatened plant Sphaerophysa kotschyana (Fabaceae): inter simple-sequence-repeat (ISSR) analysis and salt tolerance of the regenerantsen_US
dc.typeArticleen_US

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