Knockdown of lncRNA ZEB2NAT suppresses epithelial mesenchymal transition, metastasis and proliferation in breast cancer cells

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dc.contributor.authorGunes, Canan Eroglu
dc.contributor.authorGuclu, Ebru
dc.contributor.authorVural, Hasibe
dc.contributor.authorKurar, Ercan
dc.date.accessioned2024-02-23T14:02:58Z
dc.date.available2024-02-23T14:02:58Z
dc.date.issued2021
dc.departmentNEÜen_US
dc.description.abstractBreast cancer is the second most common cause of cancer-related mortality in women. Breast cancer metastasis which usually is observed at the last stage is the major cause of breast cancer-related death. Long non-coding RNAs (lncRNAs) are member of the non-coding RNA family. It is known that lncRNAs have important functions in the genes regulation of different processes and pathways such as EMT (Epithelial mesenchymal transition), metastasis and apoptosis. Therefore, it is inevitable that lncRNAs have potential contribution for the understanding of cancer pathogenesis. lncRNA-ZEB2NAT is the natural antisense transcript of ZEB2. Herein, we investigated the effects of lncRNA-ZEB2NAT on process of EMT, metastasis and apoptosis in MCF7 and MDA-MB231 breast cancer cells. The effect of ZEB2NAT on the expression of important genes in EMT, metastasis and apoptosis, and some protein levels was determined by qRT-PCR and western blot analysis, respectively. The effects of ZEB2NAT on cell proliferation, apoptosis, invasion and colony formation were evaluated using XTT, annexin V, invasion and colony assays, respectively. The ZEB2NAT knockdown caused anti-metastatic and apoptotic effects. The ZEB2NAT knockdown resulted in a decrease in ZEB2 and N-cadherin but an increase in E-cadherin protein levels. In addition, it was determined that ZEB2NAT knockdown significantly decreased cell proliferation and stimulated apoptosis in both cells. It was found that ZEB2NAT knockdown significantly decreased invasion and colony formation in both cells. ZEB2NAT knockdown showed anti-metastatic and apoptotic effect by affecting the important genes in both cells. These results have suggested that ZEB2NAT has an important role in EMT, metastasis and apoptosis in breast cancer and ZEB2NAT knockdown caused significant anti-cancer activities.en_US
dc.description.sponsorshipNecmettin Erbakan University, Scientific Research Projects [171418001]en_US
dc.description.sponsorshipThis study was produced from the PhD dissertation of C.E.G. and supported by Necmettin Erbakan University, Scientific Research Projects (#171418001).en_US
dc.identifier.doi10.1016/j.gene.2021.145904
dc.identifier.issn0378-1119
dc.identifier.issn1879-0038
dc.identifier.pmid34418470en_US
dc.identifier.scopus2-s2.0-85113464005en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.gene.2021.145904
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11924
dc.identifier.volume805en_US
dc.identifier.wosWOS:000691136800001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofGeneen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApoptosisen_US
dc.subjectBreast Canceren_US
dc.subjectEmten_US
dc.subjectMetastasisen_US
dc.subjectZeb2naten_US
dc.titleKnockdown of lncRNA ZEB2NAT suppresses epithelial mesenchymal transition, metastasis and proliferation in breast cancer cellsen_US
dc.typeArticleen_US

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