Investigation of interferon-tau stimulated genes (ISGs) simultaneously in the endometrium, corpus luteum (CL) and peripheral blood leukocytes (PBLs) in the preluteolytic stage of early pregnancy in ewes

Basit Öğe Kaydı
dc.contributor.authorKiyma, Zekeriya
dc.contributor.authorKose, Mehmet
dc.contributor.authorAtli, Mehmet Osman
dc.contributor.authorOzel, Caglayan
dc.contributor.authorHitit, Mustafa
dc.contributor.authorSen, Gonca
dc.contributor.authorKaya, Muhammet
dc.date.accessioned2024-02-23T14:16:16Z
dc.date.available2024-02-23T14:16:16Z
dc.date.issued2016
dc.departmentNEÜen_US
dc.description.abstractThe aim of the present study was to investigate the expression profiles of Interferon-tau (IFN-T) stimulated genes (ISGs) at the mid-luteal stage of the cycle (on day 13) in the uterine endometrium and extra-uterine tissues, such as the corpus luteum (CL), and in the peripheral blood leukocytes (PBLs) of pregnant and non -pregnant ewes, The objective was to evaluate the possibility of using the regulation of ISG expression in PBLs as a possible early pregnancy indicator in ruminants. For this purpose, multiparous ewes were synchronized and either allowed to mate (pregnancy group) or detected in estrus (cyclic group; day 0). The ewes were slaughtered on day 13, and the PBLs, endometrium and luteal tissues were collected. Total RNA was isolated from eight cyclic and eight pregnant ewes, and qPCR was employed to detect the steady state levels of Interferon -stimulated gene 15 (ISG15), Myxovirus (influenza virus) resistance 1 (Mxl) and Receptor transporter protein 4 (RTP4) mRNAs. The expressions of ISG15, Mxl and RTP4 were detected in the endometrium, CL and PBLs on day 13 of the estrous cycle and pregnancy. The expressions of these ISGs were upregulated only in the endometrium of pregnant ewes compared to non-pregnant ewes, but this stimulation was not observed in the CL and PBLs. The results suggest that the embryo stimulates ISGs only in the endometrium, and the effects are not evident in the extra-uterine tissues on day 13 of pregnancy. This study suggests that the measurement of the ISG expression in the PBLs is not a reliable detection method of early pregnancy in ewes, which are in the preluteolytic stage of early pregnancy. (C) 2016 Elsevier B.V. All rights reserved.en_US
dc.description.sponsorshipTUBITAK through the Scientific and Technological Research Projects Funding Program [112R022]; Selcuk University BAP [14401118, 15401029]en_US
dc.description.sponsorshipThis study was partially funded by a grant from TUBITAK through the Scientific and Technological Research Projects Funding Program (Project No: 112R022 to Z.K.) and by Selcuk University BAP (14401118 and 15401029 to A.G.). The support provided by these programs is gratefully appreciated.en_US
dc.identifier.doi10.1016/j.smallrumres.2016.05.007
dc.identifier.endpage6en_US
dc.identifier.issn0921-4488
dc.identifier.issn1879-0941
dc.identifier.scopus2-s2.0-84969822631en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage1en_US
dc.identifier.urihttps://doi.org/10.1016/j.smallrumres.2016.05.007
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12596
dc.identifier.volume140en_US
dc.identifier.wosWOS:000380603800001en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofSmall Ruminant Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectInterferon-Stimulated Genesen_US
dc.subjectPregnancyen_US
dc.subjectEwesen_US
dc.titleInvestigation of interferon-tau stimulated genes (ISGs) simultaneously in the endometrium, corpus luteum (CL) and peripheral blood leukocytes (PBLs) in the preluteolytic stage of early pregnancy in ewesen_US
dc.typeArticleen_US

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu