The influence of rifamycin decontamination on incorporation of autologous onlay bone grafts in rats: A histometric and immunohistochemical evaluation

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dc.contributor.authorTasdemir, Ufuk
dc.contributor.authorOzec, Ilker
dc.contributor.authorEsen, Haci Hasan
dc.contributor.authorAvunduk, M. Cihat
dc.date.accessioned2024-02-23T14:02:08Z
dc.date.available2024-02-23T14:02:08Z
dc.date.issued2015
dc.departmentNEÜen_US
dc.description.abstractObjective: Although it has been shown that rifamycin is an effective agent for bone graft decontamination, no information exists on the effects of rifamycin decontamination on bone graft incorporation. The aim of this study was to evaluate the influence of rifamycin decontamination on the incorporation of autologous onlay bone grafts quantitatively. Design: In 30 rats, a standardized 5.0-mm-diameter bone graft was harvested from the right mandibular angle, contaminated with saliva, decontaminated with rifamycin solution, and augmented to the left as an onlay graft. Ten animals were sacrificed at 7, 14, and 21 days after surgery. In the control group (10 rats), the onlay grafts were neither contaminated nor decontaminated, and the rats were sacrificed at 21 days after surgery. Histological slides were prepared from each grafted site for both immunohistochemistry analysis (bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) antibodies) and histometric analysis. Images obtained from the graft incorporation area with the light microscope were transferred to a PC, and theywere evaluated using Clemex PE 3.5 image analysis software. Results: The grafts were fully incorporated in all specimens. The results showed that rifamycin decontamination has no detrimental effect on graft incorporation and the findings revealed a tendency for earlier revascularization and osteogenesis in the decontamination group. Data were analyzed using variance analysis and Tukey's test. Conclusions: Rifamycin decontamination has no detrimental effect on autogenous graft incorporation, and it can be used for graft decontamination with confidence. (C) 2014 Elsevier Ltd. All rights reserved.en_US
dc.description.sponsorshipScientific Research Project Fund of Cumhuriyet University [DIS-074]en_US
dc.description.sponsorshipThis work was supported by the Scientific Research Project Fund of Cumhuriyet University under Project number DIS-074.en_US
dc.identifier.doi10.1016/j.archoralbio.2014.12.010
dc.identifier.endpage729en_US
dc.identifier.issn0003-9969
dc.identifier.issn1879-1506
dc.identifier.issue5en_US
dc.identifier.pmid25748392en_US
dc.identifier.scopus2-s2.0-84924184391en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage724en_US
dc.identifier.urihttps://doi.org/10.1016/j.archoralbio.2014.12.010
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11602
dc.identifier.volume60en_US
dc.identifier.wosWOS:000352664400007en_US
dc.identifier.wosqualityQ2en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltden_US
dc.relation.ispartofArchives Of Oral Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectAutogenous Bone Block Graften_US
dc.subjectDecontaminationen_US
dc.subjectRifamycinen_US
dc.titleThe influence of rifamycin decontamination on incorporation of autologous onlay bone grafts in rats: A histometric and immunohistochemical evaluationen_US
dc.typeArticleen_US

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