Synthesis of Carbazole-Substituted thiosemicarbazone and its Cu(II) Complex, DNA/Protein Binding, Cytotoxic, antiproliferative activities and molecular docking studies

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dc.contributor.authorFindik, Mukerrem
dc.contributor.authorKuzu, Burak
dc.contributor.authorPehlivanoglu, Suray
dc.contributor.authorKaya, Serdal
dc.contributor.authorSayin, Ulku
dc.contributor.authorAkgemci, Emine Guler
dc.contributor.authorSaf, Ahmet Ozgur
dc.date.accessioned2024-02-23T14:03:16Z
dc.date.available2024-02-23T14:03:16Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractIn this study, 9-ethyl-3-carbazolecarboxaldehyde-4-ethyl-thiosemicarbazone (ECCAET) and its copper(II) com-plex (Cu(ECCAET)2) were firstly synthesized and characterized. DFT and EPR studies confirmed that the complex is mononuclear and has square planar geometry. The interaction of all synthesized compounds with calf thymus DNA (CT-DNA) was examined by absorption and fluorescent spectroscopy. The experimental results showed that Cu(ECCAET)2 interacts with DNA via an intercalative binding mode. The binding interactions of the complex with CT-DNA have been confirmed through viscosity measurements revealing that the complex interacts with DNA via intercalation. Furthermore, the protein binding ability of ECCAET and Cu(ECCAET)2 was investigated using BSA via electronic absorption spectral titration, fluorescence quenching, and synchronous fluorescence spectrum studies, which revealed that the Cu(ECCAET)2 strongly bound to BSA over the ligand. Molecular docking studies were also performed to support the bonding mechanism of ECCAET and Cu(ECCAET)2 with DNA and BSA. The biological activity studies of ECCAET and Cu(ECCAET)2 against cancer cells were also investigated. A panel of cancer cell lines, including A2780 human ovarian adenocarcinoma, MDA-MB-231 human triple-negative breast adenocarcinoma, and as a control non-cancerous L929 fibroblast cell lines were also used to test the compounds' anticancer activities. Cytotoxic and antiproliferative properties of Cu(ECCAET)2 were visibly higher than its ligand (ECCAET) for all tested cell lines. The Cu(ECCAET)2 had a distinctive biological effects on A2780, and MDA-MB-231 cells compared to non-cancerous cells. Within these results, Cu(ECCAET)2 was found a promising drug candidate against gynecologic cancer diseases.en_US
dc.description.sponsorshipNecmettin Erbakan University [2020MER03002]en_US
dc.description.sponsorshipThe authors sincerely thank Dr. M. Abdullah Alagoz (researcher at Inonu University) for his contribution to the docking calculations. S.K. acknowledges the Necmettin Erbakan University for the financial support (Grant No: 2020MER03002) .en_US
dc.identifier.doi10.1016/j.inoche.2023.110711
dc.identifier.issn1387-7003
dc.identifier.issn1879-0259
dc.identifier.scopus2-s2.0-85153526887en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.inoche.2023.110711
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12044
dc.identifier.volume152en_US
dc.identifier.wosWOS:000983837800001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofInorganic Chemistry Communicationsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectCu(Ii) Complex Of Thiosemicarbazoneen_US
dc.subjectTd-Dften_US
dc.subjectEpren_US
dc.subjectDnaen_US
dc.subjectBsa Interactionen_US
dc.subjectMolecular Dockingen_US
dc.subjectCytotoxicityen_US
dc.subjectAntiproliferative Activityen_US
dc.subjectOvarian Canceren_US
dc.titleSynthesis of Carbazole-Substituted thiosemicarbazone and its Cu(II) Complex, DNA/Protein Binding, Cytotoxic, antiproliferative activities and molecular docking studiesen_US
dc.typeArticleen_US

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