Effective anticancer agents based-on two Pillar[5]arene derivatives for pancreas cancer cell lines: synthesis, apoptotic effect, caspase pathway

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dc.contributor.authorKaraselek, Mehmet Ali
dc.contributor.authorKuccukturk, Serkan
dc.contributor.authorDuran, Tugce
dc.contributor.authorKursunlu, Ahmed Nuri
dc.contributor.authorOzmen, Mustafa
dc.contributor.authorBozdag, Ceren
dc.contributor.authorAlkan, Selman
dc.date.accessioned2024-02-23T13:56:07Z
dc.date.available2024-02-23T13:56:07Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractThis study aimed to evaluate the possible anticancer effects of two different pillar[5]arene derivatives (5Q-[P5] and 10Q-P[5]) on two different pancreatic cancer cell lines in vitro. For this purpose, changes in the expression of major genes that play a role in apoptosis and caspase pathways were investigated. Panc-1 and BxPC-3 cell lines were used in the study and the cytotoxic dose of pillar[5]arenes was determined by the MTT method. Changes in gene expression after pillar[5]arenes treatment were evaluated by real-time polymerase chain reaction (qPCR). Apoptosis was studied by flow cytometry. As a result of analysis, it was determined that proapoptotic genes and genes involved in major caspase activation were upregulated and antiapoptotic genes were down-regulated in Panc-1 cell line treated with pillar[5]arenes. Flow cytometric apoptosis analysis also showed an increased apoptosis rate in this cell line. On the contrary, although MTT analysis showed cytotoxic effect in BxPC-3 cell line treated with two pillar[5]arene derivatives, the apoptosis pathway was not active. This suggested that it may activate different death pathways for BxPC-3 cell line. Thus, it was first determined that the pillar[5]arene derivatives reduced cancer cell proliferation on pancreatic cancer cells.en_US
dc.description.sponsorshipNecmettin Erbakan University [:221218015]en_US
dc.description.sponsorshipAcknowledgementsAuthors thank Necmettin Erbakan University (Project Number:221218015) for this study.en_US
dc.identifier.doi10.1007/s10637-023-01343-w
dc.identifier.endpage209en_US
dc.identifier.issn0167-6997
dc.identifier.issn1573-0646
dc.identifier.issue2en_US
dc.identifier.pmid36905565en_US
dc.identifier.scopus2-s2.0-85149730205en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage202en_US
dc.identifier.urihttps://doi.org/10.1007/s10637-023-01343-w
dc.identifier.urihttps://hdl.handle.net/20.500.12452/11071
dc.identifier.volume41en_US
dc.identifier.wosWOS:000946650100001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherSpringeren_US
dc.relation.ispartofInvestigational New Drugsen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectPancreas Canceren_US
dc.subjectPillar[5]Arenen_US
dc.subjectPanc-1en_US
dc.subjectBxpc-3en_US
dc.titleEffective anticancer agents based-on two Pillar[5]arene derivatives for pancreas cancer cell lines: synthesis, apoptotic effect, caspase pathwayen_US
dc.typeArticleen_US

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