The involvement of Antifreeze protein maxi-like and Cold-shock domain-containing protein genes in cold-induced larval diapause and cold-shock treatment of khapra beetle

Basit Öğe Kaydı
dc.authoridAslı Dağeri: 0000-0001-8564-9922en_US
dc.contributor.authorDağeri, Aslı
dc.contributor.authorKadir, Mohammed Lengichow
dc.contributor.authorGüz, Nurper
dc.contributor.authorÖğreten, Ayhan
dc.contributor.authorArshad, Muhammad
dc.date.accessioned2023-06-01T09:03:12Z
dc.date.available2023-06-01T09:03:12Z
dc.date.issued2023en_US
dc.departmentNEÜ, Fen Fakültesi, Moleküler Biyoloji ve Genetiken_US
dc.descriptionMakaleen_US
dc.descriptionWOS:000921012600001en_US
dc.description.abstractThe khapra beetle, Trogoderma granarium (Everts) (Coleoptera: Dermestideae), is a cosmopolitan and one of the most destructive pests of various stored grains and grain products. This pest is categorized as a quarantine threat in many countries. The larvae undergo diapause that lasts for several years depending on different stress conditions such as temperature, insecticidal applications, starvation, humidity, crowding, and fecal pellets. When conditions are favorable for the development, diapause terminates and the larva continues its development. In the present study, two cold-regulated genes Antifreeze protein maxi-like (TgAFP) and Cold-shock domain-containing protein E1 (TgCSDP) were identified in the cDNA library of T. granarium. Nucleotide sequences of two unigene cDNAs encoding TgAFP and TgCSDP were verified using RT-PCR and RACE-PCR amplifications. Transcriptional regulations of TgAFP and TgCSDP were examined in cold-induced diapause larvae, cold-shocked larvae, and at different developmental stages. Gene expression pattern of TgAFP revealed the highest mRNA levels during pre-diapause (25 degrees C) followed by the larvae exposed to 5 degrees C during the diapause phase. This can be attributable to the protective role of TgAFP against temperature fall. Significant upregulation of TgCSDP at 15 degrees C might indicate its probable chaperone role in toleration for cold-induced diapause. TgAFP level was downregulated after cold-shock treatment (CST), while it was slightly upregulated by recovery, indicating that it might confer tolerance for the recovery period (RP). The highest TgCSDP expression after the CST might suggest its involvement in response to acute CST. Low abundance of TgAFP expression in each developmental stage might suggest that 33 degrees C temperature does not induce the synthesis of TgAFP. Significant amount of TgCSDP levels in adults might indicate its putative role in development. These findings suggest that TgAFP and TgCSDP genes might be crucial for cold survival where the T. granarium undergoes its facultative diapause.en_US
dc.description.sponsorshipScientific Research Projects (BAP) Coordination Unit of Necmettin Erbakan Universityen_US
dc.identifier.citationDağeri, A., Kadir, M. L., Güz, N., Öğreten, A., Arshad, M. (2023). The involvement of Antifreeze protein maxi-like and Cold-shock domain-containing protein genes in cold-induced larval diapause and cold-shock treatment of khapra beetle. Journal of Stored Products Research, 101, 1-8.en_US
dc.identifier.doi10.1016/j.jspr.2022.102074en_US
dc.identifier.endpage8en_US
dc.identifier.issn0022-474Xen_US
dc.identifier.issn1879-1212en_US
dc.identifier.issue-en_US
dc.identifier.scopusqualityQ1en_US
dc.identifier.startpage1en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.jspr.2022.102074
dc.identifier.urihttps://hdl.handle.net/20.500.12452/9753
dc.identifier.volume101en_US
dc.identifier.wosWOS:000921012600001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherPergamon-Elsevier Science Ltd.en_US
dc.relation.ispartofJournal of Stored Products Researchen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/embargoedAccessen_US
dc.subjectAntifreeze Proteinen_US
dc.subjectCold-shocken_US
dc.subjectCold-shock Domainen_US
dc.subjectCold-shock Domain-containing Proteinen_US
dc.subjectDiapauseen_US
dc.subjectKhapra Beetleen_US
dc.titleThe involvement of Antifreeze protein maxi-like and Cold-shock domain-containing protein genes in cold-induced larval diapause and cold-shock treatment of khapra beetleen_US
dc.typeArticleen_US

Dosyalar

Orijinal paket
Listeleniyor 1 - 1 / 1
Küçük Resim
İsim:
The involvement of Antifreeze protein maxi-like and Cold-shock domain-containing protein genes in cold-induced larval diapause and cold-shock treatment of khapra beetle.pdf
Boyut:
2.91 MB
Biçim:
Adobe Portable Document Format
Açıklama:
Tam Metin (Full Text)
İndir
Lisans paketi
Listeleniyor 1 - 1 / 1
Küçük Resim Yok
İsim:
license.txt
Boyut:
1.44 KB
Biçim:
Item-specific license agreed upon to submission
Açıklama:
İndir

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Makale Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu