Apelin triggers macrophage polarization to M2 type in head and neck cancer

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dc.contributor.authorCelik, Fatma Secer
dc.contributor.authorGunes, Canan Eroglu
dc.contributor.authorYavuz, Emine
dc.contributor.authorKurar, Ercan
dc.date.accessioned2024-02-23T14:03:15Z
dc.date.available2024-02-23T14:03:15Z
dc.date.issued2023
dc.departmentNEÜen_US
dc.description.abstractCancer comes after cardiovascular diseases in terms of mortality rate in the world. Chemotherapy, radiotherapy and surgical interventions are the current cancer treatment. Recently, it has been observed that immunothera-peutic approaches provide a significant improvement when used along with these interventions. The mono-nuclear system mainly consists of macrophages that play an active role in the pathology of many diseases because of having high plasticity capacities. Previous research suggested that they can be used as an alternative to cancer treatment. Aim was to investigate the effect of apelin on macrophage polarization in the tumor microenvironment.Mouse macrophage cell line RAW264.7 cells and head and were chosen for this study. The apelin expression was knockdown in neck cell carcinoma cell line SCCL MT1 cells using shRNA technique. SCCL MT1 cells having normal or suppressed apelin expression were co-cultured with mouse macrophage RAW264.7 cells. The effect of co-culturing on the expression of inflammatory genes in RAW264.7 cells was investigated.Suppressed apelin expression in SCCL MT1 cells resulted in elevated pro-inflammatory response in co-cultured macrophages. Expression of the IL113, IL6, and TNF alpha genes significantly increased, however anti-inflammatory cytokine levels were significantly decreased. However, in the control group, a downregulation was deter-mined in pro-inflammatory genes, while an increase was observed in anti-inflammatory genes. The protein levels of these cytokines in concordance with the RT-PCR analysis.As a result of this study, apelin released from cancer cells was found to affect macrophage polarization. These results indicated that the apelin peptide may cause the intense presence of M2-type macrophages in the tumor niche, and the therapeutic approaches targeting of apelin in cancer cells may have a potential role in macrophage polarization.en_US
dc.description.sponsorshipNecmettin Erbakan University, Scien-tific Research Projects [201418006]en_US
dc.description.sponsorshipFunding source This study was supported by Necmettin Erbakan University, Scien-tific Research Projects (#201418006) .en_US
dc.identifier.doi10.1016/j.imbio.2023.152353
dc.identifier.issn0171-2985
dc.identifier.issn1878-3279
dc.identifier.issue2en_US
dc.identifier.pmid36805859en_US
dc.identifier.scopus2-s2.0-85148340193en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.urihttps://doi.org/10.1016/j.imbio.2023.152353
dc.identifier.urihttps://hdl.handle.net/20.500.12452/12035
dc.identifier.volume228en_US
dc.identifier.wosWOS:000951466300001en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakPubMeden_US
dc.language.isoenen_US
dc.publisherElsevier Gmbhen_US
dc.relation.ispartofImmunobiologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectApelinen_US
dc.subjectCanceren_US
dc.subjectHead And Necken_US
dc.subjectMacrophageen_US
dc.subjectShrnaen_US
dc.titleApelin triggers macrophage polarization to M2 type in head and neck canceren_US
dc.typeArticleen_US

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