Ginnalin A and SB203580 show additive effect on Hep-3B hepatocellular carcinoma cell line

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dc.contributor.authorVural, Hasibe
dc.contributor.authorOzden, Pinar
dc.contributor.authorAvci, Ebru
dc.date.accessioned2024-02-23T14:29:52Z
dc.date.available2024-02-23T14:29:52Z
dc.date.issued2019
dc.departmentNEÜen_US
dc.description.abstractObjective: Investigation of the anticarcinogenic effects of natural products with low toxicity is very important in the development of new therapeutic strategies against cancer. Ginnalin A (GA) is one of the most important phenolic compounds of Acer genus and its anticancer effect has been shown that in various cancer cell lines. SB203580, a p38 MAPK inhibitor, can inhibit cell proliferation independently of p38 MAPK. The objective of this study was to investigate combination effect of GA and SB203580 on Hep-3B cell line. Material and methods: Cell viability was determined by using XTT method after the treatment with GA, SB203580 and combination of both. Anticarcinogenic effects of GA and SB203580 both in single and in combination have been analyzed with Caspase-3 activity assay and expression levels of important genes involved in cell cycle and apoptosis were evaluated by qPCR. Results: GA and SB203580 have shown additive effect on Hep-3B cells in the combination inhibited 50% of cell viability. And, SB203580 increased the effect of GA on activation of Caspase-3 and expressions of genes important in apoptosis and cell cycle. Conclusion: This study indicates that GA and SB203580 can be an effective for development of new therapeutic strategies in hepatocellular carcinoma.en_US
dc.description.sponsorshipN.E.U. Scientific Research Projects (BAP) [171318001]en_US
dc.description.sponsorshipThis study was produced from the MSc thesis of Pinar Ozden and supported by N.E.U. Scientific Research Projects (BAP #171318001).en_US
dc.identifier.doi10.1515/tjb-2018-0099
dc.identifier.endpage85en_US
dc.identifier.issn0250-4685
dc.identifier.issn1303-829X
dc.identifier.issue1en_US
dc.identifier.scopus2-s2.0-85064713602en_US
dc.identifier.scopusqualityQ4en_US
dc.identifier.startpage78en_US
dc.identifier.urihttps://doi.org/10.1515/tjb-2018-0099
dc.identifier.urihttps://hdl.handle.net/20.500.12452/14903
dc.identifier.volume44en_US
dc.identifier.wosWOS:000461427300011en_US
dc.identifier.wosqualityQ4en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.language.isoenen_US
dc.publisherWalter De Gruyter Gmbhen_US
dc.relation.ispartofTurkish Journal Of Biochemistry-Turk Biyokimya Dergisien_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAdditive Effecten_US
dc.subjectCaspase-3 Activityen_US
dc.subjectGinnalin Aen_US
dc.subjectHepatocellular Carcinomaen_US
dc.subjectSb203580en_US
dc.titleGinnalin A and SB203580 show additive effect on Hep-3B hepatocellular carcinoma cell lineen_US
dc.typeArticleen_US

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