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    A Dicationic BODIPY-Based Fluorescent Bactericide to Combat Infectious Diseases and to Eradicate Bacterial Biofilms
    (Amer Chemical Soc, 2023) Kocak, Haluk Samet; Bulut, Onur; Yilmaz, M. Deniz
    Increased bacterial resistance against extensively used common disinfectants has begun to emerge. The discovery of disinfectants substituting the current commercially available ones is strongly needed. For this purpose, a dicationic BODIPY-based fluorescent amphiphile has been synthesized by specific molecular design. This quaternized BODIPY behaves as a broad-spectrum disinfectant against both Gram-positive and Gram-negative bacteria strains. It exhibits potent antimicrobial activity against tested microorganisms when compared with structurally similar disinfectant benzalkonium chloride (BAC). Moreover, it shows antibiofilm activity against Staphylococcus epidermidis with a minimum biofilm eradication concentration as low as 16 mu g/mL. The interaction of this compound with the bacterial cell and genomic DNA was further evaluated by fluorescence spectroscopy and microscopy to follow cell internationalization and to clarify the mechanism of antibacterial action.
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    Fluorescent silica nanoparticles as nano-chemosensors for the sequential detection of Pb2+ions and bacterial-spore biomarker dipicolinic acid (DPA) in aqueous solution
    (Pergamon-Elsevier Science Ltd, 2023) Cetinkaya, Yagmur Nur; Bulut, Onur; Oktem, Huseyin Avni; Yilmaz, M. Deniz
    Herein, we report fluorescein-labelled silica nanoparticles (FSNP) which serve as fluorescent nano-chemosensors for sequential detection of Pb2+ (which is a toxic heavy metal) and dipicolinic acid (DPA) (which is a distinctive indicator biomarker of bacterial spores) with high sensitivity and selectivity. The fluorescence of FSNP is quenched because of the complex formation between Pb2+ ions and surface amide groups, however, the fluorescence is recovered in contact with DPA, resulting from the association of DPA with surface bound Pb2+ ions. FSNP-Pb2+ complexes show high sensitivity towards DPA with a low detection limit of 850 nM which is approximately seventy times lower than the infectious dosage of bacterial spores (60 & mu;M). Lateral flow test platform was further developed to show the applicability and practicability of our system.