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Öğe Ameliorating the effects of Adalimumab on rabbits with experimental cerebral vasospasm after subarachnoid hemorrhage(Turkish Assoc Trauma Emergency Surgery, 2020) Toguslu, Gokhan; Erdi, Mehmet Fatih; Arac, Densel; Keskin, Fatih; Kilinc, Ibrahim; Cuce, GokhanBACKGROUND: Adalimumab (ADA), which is a new-generation recombinant human monoclonal antibody for tumor necrosis factor a (TNF alpha), has strong anti-inflammatory effects. The role of enhanced inflammation is well established for the development and progression of cerebral vasospasm. Investigated in the present study is the probable ameliorating and neuroprotective effects of ADA in rabbits using a cerebral vasospasm model with biochemical and histopathological methods. METHODS: Thirty male New-Zealand white rabbits were randomly divided into control, subarachnoid hemorrhage (SAH) only and SAH plus ADA treatment groups. SAH was established as a single cisterna magna autologous arterial blood injection. ADA treatment was started just after intracisternal blood injection and continued for 72 hours once a day. The animals were sacrificed 72 hours after the induction of SAH, serum and brainstem tissue obtained for investigations. RESULTS: Brainstem tissue and plasma levels of tumor necrosis factor-alpha and Interleukin-1 beta, brainstem tissue Matrix metalloproteinase-9 levels increased after SAH and partly decreased after treatment. Plasma levels of brain-derived neurotrophic factor decreased after SAH and partly restored after treatment. ADA treatment significantly increased the mean cross-sectional area of the vasospastic basilar arteries, reduced the basilar artery wall thickness and also ameliorates enhanced endothelial apoptosis. CONCLUSION: Findings obtained in this study suggest that ADA is an effective neuroprotective agent for ameliorating cerebral vasospasm in experimental rabbit vasospasm.Öğe Ameliorating the effects of Adalimumab on rabbits with experimental cerebral vasospasm after subarachnoid hemorrhage(Turkish Assoc Trauma Emergency Surgery, 2020) Toguslu, Gokhan; Erdi, Mehmet Fatih; Arac, Densel; Keskin, Fatih; Kilinc, Ibrahim; Cuce, GokhanBACKGROUND: Adalimumab (ADA), which is a new-generation recombinant human monoclonal antibody for tumor necrosis factor a (TNF alpha), has strong anti-inflammatory effects. The role of enhanced inflammation is well established for the development and progression of cerebral vasospasm. Investigated in the present study is the probable ameliorating and neuroprotective effects of ADA in rabbits using a cerebral vasospasm model with biochemical and histopathological methods. METHODS: Thirty male New-Zealand white rabbits were randomly divided into control, subarachnoid hemorrhage (SAH) only and SAH plus ADA treatment groups. SAH was established as a single cisterna magna autologous arterial blood injection. ADA treatment was started just after intracisternal blood injection and continued for 72 hours once a day. The animals were sacrificed 72 hours after the induction of SAH, serum and brainstem tissue obtained for investigations. RESULTS: Brainstem tissue and plasma levels of tumor necrosis factor-alpha and Interleukin-1 beta, brainstem tissue Matrix metalloproteinase-9 levels increased after SAH and partly decreased after treatment. Plasma levels of brain-derived neurotrophic factor decreased after SAH and partly restored after treatment. ADA treatment significantly increased the mean cross-sectional area of the vasospastic basilar arteries, reduced the basilar artery wall thickness and also ameliorates enhanced endothelial apoptosis. CONCLUSION: Findings obtained in this study suggest that ADA is an effective neuroprotective agent for ameliorating cerebral vasospasm in experimental rabbit vasospasm.Öğe Chemoprotective effect of vitamin E in cyclophosphamide-induced hepatotoxicity in rats(Elsevier Ireland Ltd, 2015) Cuce, Gokhan; Cetinkaya, Seda; Koc, Tugba; Esen, Haci Hasan; Limandal, Cisem; Balci, Tevfik; Kalkan, SerpilCyclophosphamide (CP) has a range of adverse effects on liver tissue in humans and animals. Administering an antioxidant with CP might reduce such side effects. Therefore, we examined the role of vitamin E in CP-induced liver toxicity in rats. Male Wistar albino rats were divided into four groups, each of seven rats: control, CP only, CP + vitamin E, and vitamin E only groups. The rats were administered treatments intraperitoneally for 7 days. Then the serum malondialdehyde (MDA), alanine aminotransferase (ALT), aspartate transaminase (AST), and lactate dehydrogenase (LDH) levels were determined while the livers were removed, tissue was prepared using routine histological procedures, sections were stained using hematoxylin and eosin, and the terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL) method was applied. Histopathologically, CP caused hydropic degeneration, necrosis, pleomorphism, and mitotic activity. The number of TUNEL-positive cells and the MDA and ALT levels were significantly higher in the CP group. The antioxidant effects of vitamin E significantly decreased the number of TUNEL-positive cells and the ALT and MDA levels, and normalized the liver histopathology. CP induces apoptosis, has toxic effects on liver tissue, and changes the histological structure. The administration of vitamin E prevented the liver tissue damage caused by CP. (C) 2015 Elsevier Ireland Ltd. All rights reserved.Öğe Effects of Lipokit® Centrifugation on Morphology and Resident Cells of Adipose Tissue(Soc Chilena Anatomia, 2013) Duman, Selcuk; Aktan, Tahsin Murad; Cuce, Gokhan; Cihantimur, Bulent; Tokac, Mehmet; Akbulut, HabipThe aim of adipose tissue engineering is creating autologus vascularized fat tissue to be used for practical soft tissue reconstruction in human clinic. Unfortunately, in practice, long-term results of fat transplantation are often untrustworthy and unreliable, to overcome this problem different many lipoinjection techniques developed in the last 20 years. Centrifuge is a fundamental step in the preparation of adipose tissue. We focused on some cell markers especially MSCs markers and histological structural properties after with lipokit centrifugation and without lipokit centrifugation of adipose tissue obtained by liposuction by this new technique. Adipose tissue was taken by liposuction and separates to two portions. One of them is centrifugated by Lipokit machine (C+) has a micro filter and the other is not (C-). After centrifugation smear slides and paraffin sections were prepared from these tissues. These slides were stained with H&E and Toluidine Blue. Paraffin sections were immunohistochemically stained with CD34, von Willebrand Factor, CD73, CD90 and CD105. Smear preparations showed a continuous three dimensional plasma membrane appearance of adipocytes. C+ and C-showed expression of CD34, von Willebrand Factor, CD73, CD90, CD105. C+ seems to have more free cells expressing than C-. While passing the filter of Lipokit, large adipocytes and connective tissue parts disintegrate and thus increases the surface area of lipoaspirate. Lipokit (R) machine release the group cells which are necessary for angiogenesis and they become more freely to construct angiogenesis.Öğe Evaluation of Apoptosis Pathway of Geraniol on Ishikawa Cells(Routledge Journals, Taylor & Francis Ltd, 2021) Kuzu, Betul; Cuce, Gokhan; Ayan, Ilknur Cinar; Gultekin, Burcu; Canbaz, Halime Tuba; Dursun, Hatice Gul; Sahin, ZaferEndometrial cancer is the most common type of cancer in the female reproductive system. Geraniol is acyclic monoterpene alcohol derived from essential oils of aromatic plants. This study aimed to investigate the apoptosis pathway of geraniol on Ishikawa cells. The cytotoxic effects of Geraniol on Ishikawa cells were determined by an MTT test. Ishikawa cells were seeded on cover slips, the IC50 dose was applied, and the cells were incubated with antibodies against Bax, Bcl-2, and TUNEL Assay. mRNA expression analysis of apoptosis-related genes was determined by RT-qPCR with an IC50 dose of Geraniol. The IC50 dose of Geraniol decreased Bcl-2 staining significantly, but it significantly increased Bax staining and TUNEL positive cells. A significant increase in the Bax, caspase3, caspase-8, cytochrome C and Fas genes and a significant decrease in the Bcl-2 gene was observed when the IC50 dose group was compared to the cells in the control group based on their mRNA expression levels.Analysis of expression of genes whose products are involved in apoptosis suggests the involvement of the mitochondrial pathway.Öğe Evaluation of cerebrum volume of children 1-5 years old(Scientific Publishers India, 2014) Cuce, Gokhan; Seker, Muzaffer; Kalkan, Serpil; Canbilen, Aydan; Sakarya, Mehmet Emin; Cuce, Hasan; Yilmaz, Mehmet TugrulTotal brain volume and regional brain size can vary in men and women. Sexual dimorphism between the male and female brain starts in the fetal period under the influence of genetic and hormonal factors. In addition, the newborn brain begins to change in terms of both brain volume and morphology. In the present study, we evaluated gender-related changes in brain volume in children 1-5 years old. Magnetic resonance images of 30 healthy children aged 1-5 years old (15 male, 15 female) were obtained from the Department of Radiology, Meram School of Medicine, University of Necmettin Erbakan. The Cavalieri method and point-counting were used to determine the brain hemisphere volumes from these images. The mean volumes of the right cerebrum in female and male children were 423.26 +/- 110.62 cm(3) and 456.43 +/- 161.39 cm(3), respectively, and those of the left cerebrum were 416.41 +/- 103.51 cm(3) and 460.42 +/- 154.99 cm(3), respectively. There were no significant differences in cerebrum hemisphere volumes between male and female children 1-5 years old (P > 0.05).Öğe Hypothalamic Expressions of Apelin, Apelin Receptor and Neuritine in the First Generation Rat Pups: A Maternal Depression Model(Karger, 2021) Kurar, Ercan; Gunes, Canan Eroglu; Koca, Raviye Ozen; Solak, Hatice; Koc, Aynur; Sahin, Zafer; Cuce, Gokhan[Abstract Not Availabe]Öğe Investigation of the effect of naringenin on oxidative stress-related alterations in testis of hydrogen peroxide-administered rats(Wiley, 2017) Sahin, Zafer; Ozkaya, Ahmet; Cuce, Gokhan; Uckun, Mirac; Yologlu, ErtanTestis tissue is prone to oxidation because its plasma membrane contains many polyunsaturated fatty acids. Naringenin is a plant-derived natural flavonoid. We investigated the possible ameliorative role of naringenin on the hydrogen peroxide (H2O2)-induced testicular damage in Wistar rats. Animals received 12mg/kg H2O2 by intraperitoneal injection, and 50mg/kg naringenin via orogastric gavage for 4 weeks. In the H2O2 group, the testis malondialdehyde level increased, while the amount of reduced glutathione, glutathione transferase activities, and the testis weight decreased. There were severe testicular damages in the H2O2 group otherwise their grade were less in the naringenin+H2O2 group. However, the serum testosterone concentrations decreased in both the H2O2 and the naringenin+H2O2 groups. The testicular zinc and calcium levels reduced in the H2O2-treated rats. In conclusion, the administration of H2O2 caused oxidative stress in the testes and naringenin supplementation decreased the H2O2-induced effects, except for changes in testosterone levels.Öğe Neuroprotective Effects of Milrinone on Experimental Acute Spinal Cord Injury: Rat Model(Elsevier Science Inc, 2021) Arac, Densel; Erdi, Mehmet Fatih; Keskin, Fatih; Kenan, Mehmet; Cuce, Gokhan; Aydemir, Fatma H. Y.; Guney, OnderOBJECTIVE: Spinal cord injury (SCI) disrupts nerve axons with devastating neurological consequences, but there is no effective clinical treatment. The secondary damage mechanism is a mainstay process, and it starts within a few minutes after trauma. We aim to investigate the neuroprotective effects of milrinone on the SCI model. MATERIALS AND METHODS: A total of 36 Wistar albino rats, each weighing 300-400 g, were randomly split into 4 groups that received different treatments: in group 1 (sham) (n = 9) control, only a laminectomy was performed; in group 2 (SCI) (n = 9), SCI was imitated after laminectomy; in group 3 (SCI + saline) (n = 9), physiological saline solution was injected intraperitoneally immediately after the SCI; and in group 4 (SCI + milrinone), milrinone was administered intraperitoneally on lateral decubitus position immediately after the SCI. Spinal cord contusion was established by the weight-drop technique after laminectomy. Neurological examination scores were recorded, and rats were killed 72 hours later. Serum and spinal cord tissue glutathione peroxidase, total antioxidant status, total oxidant status, 8-hydroxiguanosine, interleukin-6 and interleukin-10 levels, histopathological spinal cord damage score, and apoptotic index were examined and compared between groups. RESULTS: Neurological examination scores were significantly better in the milrinone-treated group compared with groups 2 and 3. SCI significantly increased serum and spinal cord tissue glutathione peroxidase, total oxidant status, 8- hydroxiguanosine, and interleukin-6 levels that were successfully reduced with milrinone treatment. Interleukin-10 and total antioxidant status levels decreased as a result of SCI increased with milrinone treatment. Increased histopathological spinal cord damage score and apoptotic index in groups 2 and 3 significantly decreased in group 4. CONCLUSIONS: Milrinone could reduce apoptosis and increase anti-inflammatory and antioxidative mediators, thus playing a protective role in secondary nerve injury after SCI in rats.Öğe Neuroprotective Effects of Tocilizumab on Experimentally-Induced Spinal Cord Ischemia-Reperfusion Injury(Elsevier Science Inc, 2019) Karatas, Yasar; Erdi, Mehmet Fatih; Kaya, Bulent; Keskin, Fatih; Cuce, Gokhan; Kilinc, Ibrahim; Uyar, MehmetOBJECTIVES: We aimed to evaluate neuroprotective effects of tocilizumab on spinal cord ischemia-reperfusion (I/R) injury. Our study design was an experimental rabbit spinal cord I/R injury model, and the setting was at the Animal Research Laboratory, Necmettin Erbakan University, Meram School of Medicine, Konya, Turkey. METHODS: Twenty-four adult New Zealand rabbits were randomly divided into 3 groups: Group 1, control group (n = 8); Group 2, I/R group, and Group 3 (n = 8) I/R injury D tocilizumab (4 mg/kg, ip) treatment group. Spinal cord I/R injury repair was performed by infrarenal aortic cross clamping. On neurologic evaluation, spinal cord tissue plasma tumor necrosis factor alpha (TNFa), total antioxidant status (TAS), total oxidant status (TOS), thiobarbituric acid reactive substances (TBARS), interleukin 6 (IL-6), interleukin 10 (IL-10) levels were analyzed. Spinal cord neuronal damage score and apoptotic cell count were also investigated. RESULTS: I/R injury significantly increases the plasma and spinal cord tissue TNFa, TOS, TBARS, and IL-6 levels and decreases the plasma and spinal cord tissue TAS and IL-10 levels. Tocilizumab treatment significantly reduces the plasma and spinal cord tissue TNF alpha, TOS, TBARS, IL-6 levels and increases plasma and tissue TAS and IL-10 levels. I/R injury significantly increases spinal cord neuronal damage score and apoptotic cell count. Tocilizumab treatment significantly reduces spinal cord neuronal damage score and apoptotic cell count. Neurologic examination scores at 24, 48, and 72 hours were significantly better in the treatment group when compared with the I/R group. CONCLUSIONS: This study shows significant neuroprotective effects of tocilizumab on rabbit spinal cord I/R injury.Öğe Vitamin E partially ameliorates cyclophosphamide-induced nephrotoxicity in rats(Mattioli 1885, 2016) Cuce, Gokhan; Esen, Haci Hasan; Koc, Tugba; Canbaz, Halime Tuba; Limandal, Cisem; Kalkan, Serpil; Gurbilek, MehmetPurpose: Cyclophosphamide (CP) is a widely used anti chemotherapeutic drug, which causes nephrotoxicity due to its toxic metabolites. This study was carried out to assess the effects of vitamin E on cyclophosphamide induced renal toxicity in rats. Model: Twenty-eight Wistar albino rats were assigned to four groups, which were given 20 mg/kg CP, 20 mg/kg CP + 100 mg/kg vitamin E, 100 mg/kg vitamin E, or 20 mg/kg isotonic sodium chloride solution intraperitoneally each day for 7 days. Effects were assessed by histology of the kidney, TUNEL assay and measurement of serum uric acid and creatinine. Results: Cyclophosphamide significantly increased glomerular inflammation, edema, congestion and tubular degeneration, TUNEL positive cells, while addition of vitamin E significantly decreased glomerular inflammation, edema and TUNEL positive cells. Cyclophosphamide did not affect urea and creatinine levels, which may due to the absence of renal necrosis. Conclusion: Vitamin E application appears to partially ameliorate Cyclophosphamide induced renal toxicity.
