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    Evaluation of sialic acid, malondialdehyde and glutathione levels in infertile male
    (Elsevier Espana Slu, 2022) Menevse, Esma; Dursunoglu, Duygu; Cetin, Nihal; Korucu, Emine Nedime; Erbayram, Fatma Zehra
    Introduction and objectives: Around 15% of productive couples in the world are infertile. Recent years, biochemical mechanisms leads to male infertility are started to research. Redox regulation and oxidative stress (OS) show importance in the pathogenesis of infertility in male. Malondialdehyde (MDA) and Glutathione (GSH) are biochemical indicatives of sperm damage by reactive oxygen species (ROS). In addition, sperm are coated with a thick glycocalyx rich in sialic acids. It is aimed to determine and evaluate the differences between normozoosper-mic and oligozoospermic individuals according to sialic acid, MDA and GSH concentrations and correlations between spermyogram and these parameters. Material and methods: This study was carried out on seminal plasma of individuals who admit-ted to Selcuk University Faculty of Medicine IVF Unit Andrology Laboratory. The groups were divided into two as normozoospermics (n = 30, sperm concentration >= 15 million/mL), and oligozoospermics (n = 30, sperm concentration < 15 million/mL). Spermyogram were evaluated regarding WHO (2010) Kruger criteria. GSH, MDA and sialic acid concentrations were analyzed in seminal plasma. Diagnostic performance of sialic acid has been determined with ROC curve analysis. Results: Sialic acid levels were significantly lower in Normozoospermic than Oligozoosper-mic individuals (p < 0.0001), MDA and GSH levels were not differ significantly in both groups (p > 0.05). Sialic acid correlated significantly with most of the spermyogram findings. When diagnostic performance of sialic acid was evaluated, the cut off value of sialic acid found as 4.175 nmol/mL by ROC curve. Conclusion: High seminal plasma sialic acid levels may be used as a biomarker and sialic acid is important determinant in oligozoospermia. (c) 2022 Asociacion Espan tilde ola de Andrologi acute accent a, Medicina Sexual y Reproductiva. Published by Else-vier Espan tilde a, S.L.U. All rights reserved.
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    THE LEVELS OF ADENOSINE TRIPHOSPHATE SYNTHASE 6, MANGANESE SUPEROXIDE DISMUTASE, NADPH-CYTOCHROME P450 REDUCTASE, LACTATE DEHYDROGENASE IN SEMINAL PLASMA OF OLIGOZOOSPERMIA AND NORMOZOOSPERMIA MEN
    (Nobel Ilac, 2023) Menevse, Esma; Dursunoglu, Duygu; Korucu, Emine Nedime; Cetin, Nihal; Erbayram, Fatma Zehra
    Objective: In recent years, energy production and enzymes in mitochondrial pathways are an important in male fertilization.Material and Method: For this purpose, we tried to determine the relationship between normozoospermia and oligozoospermia individuals according to levels of some mitochondrial enzymes. The seminal plasma levels of mitochondrial encoded adenosine triphosphate synthase 6 (mtATP6), manganese superoxide dismutase (Mn-SOD), NADPH-cytochrome P450 reductase (CPR), lactate dehydrogenase (LDH) were analyzed and assessed their correlations between data of spermogram. The study was composed of two groups consisting of normozoospermic and oligozoospermic volunteers.Results: mtATP6 levels were significantly higher in normozoospermia (0.97 & PLUSMN;0.50 ng/mg protein, p=0.008) than oligozoospermia (0.64 & PLUSMN;0.32 ng/mg protein). LDH levels were higher in normozoospermia (0.25 & PLUSMN;0.074 U/ mg protein, p=0.007) than oligozoospermia (0.199 & PLUSMN;0.049 ng/mg protein). The groups did not differ in terms of MnSOD and CPR levels. mtATP6 levels were significantly correlated with sperm concentration, total number and total motility, immotility, total progressive motile sperm count (TPMSC) and long head anomaly. LDH levels were related with long head and short tail anomaly.Conclusion: We observed some of the spermatogenetic anomalies are caused by defects in energy regulation. We did not find any evidence on MnSOD and CPR activity regarded as determinative parameters in response to increased oxidative stress in oligozoospermia.