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Öğe Antibacterial and Antibiofilm Activity of Carboxymethyl Cellulose Stabilized Silver Nanoparticles Synthesized using Quercetin and Their Effects on Soil Respiration and Enzymes(Taylor & Francis Inc, 2022) Erci, FatihSilver nanoparticles (AgNPs) were synthesized using quercetin in aqueous carboxymethyl cellulose (CMC) solution, which is used as both the reducing and capping bioagents in the current study. The characterization of synthesized CMC-Q AgNPs was performed through different analysis techniques. Antibacterial activity was assessed by using agar well diffusion assay against both gram-positive and gram-negative strains. Also, the effect of nanoparticles on biofilm formation of Staphylococcus aureus was investigated. Moreover, soil treated with CMC-Q AgNPs and AgNO3 was analyzed in terms of soil respiration, silver contents, and soil exoenzymes (dehydrogenase and urease). In the study, the microscopic images represented the average size of the CMC-Q AgNPsto be 27 nm and the zeta potential of -59.4 (mV) could also be signed as the higher stability. Generally, the results indicate that CMC-Q AgNPs might be powerful antibacterial agents. Furthermore, we found that CMC-Q AgNPs and AgNO3 could both inhibit exoenzyme activities in soil, with the inhibitory effects especially obvious at higher exposure times and concentrations. Also, the findings revealed that Ag concentration and soil respiration (% of control) in the soil treated with AgNO3 was lower compared to CMC-Q AgNPs treatment. This might be explained in terms of different transformations of two compounds in the soil.Öğe Antimicrobial and antibiofilm activity of green synthesized silver nanoparticles by using aqueous leaf extract of Thymus serpyllum(2019) Erci, Fatih; Torlak, EmrahRecently, metal nanoparticles have attracted the attention of researchers due to their unique properties whencompared with bulk materials and have become used in many fields of application. In this study, green synthesis ofAg nanoparticles (AgNPs) was investigated by using the aqueous extract of T. serpyllum leaves. In addition,antimicrobial and antibiofilm activities of the synthesized AgNPs were evaluated in this study. Further, ultraviolet-visible spectroscopy (UV-Vis), fouirer transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) and transmission electron microscopy (TEM) were used for characterization of the green synthesized AgNPs. The UV-Vis spectrum of the synthesizedAgNPs had a maximum peak at 467 nm. Also, TEM analysis indicated spherical particles with an average size of 25.2 nm. The synthesized AgNPs have higher stability (zeta potential: -29.5 mV). The antimicrobial activity of thegreen synthesized AgNPs was investigated on both Gram-positive and Gram-negative bacteria, such as Bacilluscereus (B. cereus), Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Salmonella enterica serovarTyphimurium (S. Typhimurium) using agar well diffusion assay. According to the results of the study, Gram-positivebacteria showed larger inhibition zones compared to Gram-negative bacteria. Finally, the AgNPs were explored forthe inhibition of S. aureus biofilms. AgNPs at 100 ?g/mL concentration showed a high inhibition value of about 73%for S. aureus biofilm formation. So, it is concluded that the synthesized AgNPs might be potentially used in manyapplications due to their antimicrobial and antibiofilm properties.Öğe Bacterial contamination of coins obtained from school canteen and green market(Taylor & Francis Ltd, 2019) Yontem, Mustafa; Doyuk, Sabire A.; Erci, Fatih; Erdogdu, Behic S.[Abstract Not Availabe]Öğe Considerations on the Controlled Delivery of Bioactive Compounds through Hyaluronic Acid Membrane(Mdpi, 2022) Eftimie Totu, Eugenia; Manuc, Daniela; Totu, Tiberiu; Marilena Cristache, Corina; Buga, Roxana-Madalina; Erci, Fatih; Cristea, Camelia(1) Background: The standard treatment for periodontal disease, a chronic inflammatory state caused by the interaction between biofilms generated by organized oral bacteria and the local host defense response, consists of calculus and biofilm removal through mechanical debridement, associated with antimicrobial therapy that could be delivered either systemically or locally. The present study aimed to determine the effectiveness of a hyaluronic acid membrane matrix as a carrier for the controlled release of the active compounds of a formulation proposed as a topical treatment for periodontal disease, and the influence of pH on the complex system's stability. (2) Methods: The obtained hyaluronic acid (HA) hydrogel membrane with dispersed melatonin (MEL), metronidazole (MZ), and tetracycline (T) was completely characterized through FTIR, XRD, thermal analysis, UV-Vis and fluorescence spectroscopy, fluorescence microscopy, zeta potential and dielectric analysis. The MTT viability test was applied to check the cytotoxicity of the obtained membranes, while the microbiological assessment was performed against strains of Staphylococcus spp. and Streptococcus spp. The spectrophotometric investigations allowed to follow up the release profile from the HA matrix for MEL, MZ, and T present in the topical treatment considered. We studied the behavior of the active compounds against the pH of the generated environment, and the release profile of the bioactive formulation based on the specific comportment towards pH variation. The controlled delivery of the bioactive compounds using HA as a supportive matrix was modeled applying Korsmeyer-Peppas, Higuchi, first-order kinetic models, and a newly proposed pseudo-first-order kinetic model. (3) Results: It was observed that MZ and T were released at higher active concentrations than MEL when the pH was increased from 6.75, specific for patients with periodontitis, to a pH of 7.10, characterizing the healthy patients. Additionally, it was shown that for MZ, there is a burst delivery up to 2.40 x 10(-5) mol/L followed by a release decrease, while for MEL and T a short release plateau was recorded up to a concentration of 1.80 x 10(-5) mol/L for MEL and 0.90 x 10(-5) mol/L for T, followed by a continuous release; (4) Conclusions: The results are encouraging for the usage of the HA membrane matrix as releasing vehicle for the active components of the proposed topical treatment at a physiological pH.Öğe Effect of Tween 80 on Conjugated Linoleic Acid Production by Lactobacillus Strains in Reconstituted Skim Milk Powder(Kafkas Univ, Veteriner Fakultesi Dergisi, 2016) Torlak, Emrah; Yalcin, Suzan; Erci, FatihIn this study, three conjugated linoleic acid (CLA)-producing strains of Lactobacillus were cultured up to 36 in reconstituted skim milk powder (10%) supplemented with 2000 mu g/ml of free linoleic acid (LA) and various concentrations of Tween 80. During the incubation, total CLA levels in the culture supernatants were determined by UV-spectrophotometry. The CLA levels significantly (P<0.05) increased with the addition of 5 and 20 mg/ml Tween 80. However, increasing Tween 80 concentration from 20 to 40 mg/ml did not appear to enhance CLA levels. Similar increase patterns were observed in the growth rate and CLA production of Lactobacillus strains during the incubation.Öğe Effect of Tween 80 on Conjugated Linoleic Acid Productionby Lactobacillus Strains in Reconstituted Skim Milk Powder(2016) Torlak, Emrah; Yalçın, Suzan; Erci, FatihBu çalışmada üç adet konjuge linoleik asit (KLA) üreten Lactobacillus suşu 2000 µg/ml linoleik asit (LA) ve çeşitli konsantrasyonlarda Tween 80 ilave edilmiş rekonstitüe yağsız süt tozu (%10) içinde 36 saate kadar kültüre edilmiştir. İnkübasyon boyunca kültür süpernatantlarında toplam KLA düzeyleri UV-spektrofotometre ile tespit edilmiştir. KLA düzeyleri 5 ve 20 mg/ml Tween 80 ilavesi ile önemli (P0.05) seviyede artmıştır. Bununla birlikte, Tween 80 konsantrasyonunun 20 mg/ml'den 40 mg/ml'ye arttırılması KLA düzeyinde bir artışa neden olmamıştır. İnkübasyon esnasında Lactobacillus suşlarının gelişme hızı ve KLA üretimlerinde benzer artış oranları gözlenmiştir.Öğe Effect of ultrasound treatment on bacteriostatic activity of piezoelectric PHB-TiO2 hybrid biodegradable scaffolds prepared by electrospinning technique(Wiley, 2023) Sarripek, Fatma Bayram; Ozaytekin, Ilkay; Erci, FatihIn this study, biodegradable piezoelectric poly(3-hydroxybuthyrate) (PHB) and PHB-TiO2 as well as non-piezoelectric poly(e-caprolactone) (PCL) fibrous scaffolds were successfully fabricated. First, TiO2 nanoparticles (NPs) with various content (1%, 2%, and 3% wt) were loaded into the PHB matrix to improve its tensile and wettability properties as well as piezoelectric performances. The piezoelectric property of the fibrous scaffolds was examined and a significant improvement in the piezoelectric property of hybrid fibrous scaffolds compared to pure PHB was detected. For the PHB-2%TiO2 sample, a maximum of in the range 4.5-5 V electricity production from a height of 10 cm and a mass drop of 35 g was observed after 150 degrees C heat treatment. Then, an in vitro bactericidal analysis was carried out to test the bacteriostatic effect of the produced piezoelectric biomaterials against E-cherichia coli (E coli) under ultrasound treatment. It was observed that E. coli appeared to be the most sensitive to the PHB-%2TiO(2) sample and consequently the antibacterial activity of all the samples against E. coli was dependent on the piezoelectric properties of the samples. The results indicated that the fabricated fibrous scaffolds could be considered as a promising piezoelectric biomaterial with ultrasonically-controlled bacteriostatic activity for various tissue engineering applications.Öğe Efficacy of gaseous ozone for reducing microflora and foodborne pathogens on button mushroom(Elsevier Science Bv, 2015) Akata, Ilgaz; Torlak, Emrah; Erci, FatihDuring growth, mushrooms can be contaminated with both saprophytic and pathogenic microorganisms derived from various points of contamination. This study was performed to evaluate the efficacy of gaseous ozone for reduction of microbial load and elimination of Salmonella, Listeria monocytogenes and Escherichia colt O157:H7 on white button mushroom (Agaricus bisporus (J.E. Lange) Imbach). Whole mushroom samples were exposed to gaseous ozone up to 60 mm at concentrations of 2.8 and 5.3 mg L-1. The level of yeast and mold population naturally present on mushrooms was reduced more than 1.43 log after ozonation at 5.3 mg L-1 for 45 mm. Exposure to ozone at 2.8 and 5.3 mg L-1 for 60 min yielded 2.44 and 3.07 log reductions in aerobic plate counts, respectively. Initial levels of Salmonella, L monocytogenes and E. coli O157:H7 populations on inoculated mushrooms reduced by ranging 2.10 and 2.76 log after 60 min of treatment performed at concentration of 2.8 mg L-1, respectively. Ozonation at 5.3 mg L-1 for 60 mm reduced the initial counts of Salmonella, L monocytogenes and E. coli O157:H7 by 3.61, 2.80 and over 3.41 log, respectively. These results suggest that gaseous ozone treatment can improve the microbial safety and postharvest quality of mushrooms. (C) 2015 Elsevier B.V. All rights reserved.Öğe Fabrication of antimicrobial poly(3-hydroxybuthyrate)/poly (?-caprolactone) nanofibrous mats loaded with curcumin/?-cylodextrin inclusion complex as potential wound dressing(Elsevier, 2023) Erci, Fatih; Sariipek, Fatma BayramIn recent years, bioactive natural agent-based wound dressings have gained a lot of interest thanks to their biocompatibility, non-toxicity, and antibacterial properties. In this study, poly(3-hydroxybutyrate)/poly(epsilon-cap-rolactone) (PHB/PCL) nanofibrous mats loaded with curcumin-beta cyclodextrin (CUR-CD) inclusion complex, a bioactive agent, were successfully developed using the electrospinning technique. First, the CUR-CD inclusion complex was fabricated and characterized by FT-IR, XRD and UV-Vis analyses. This complex was then incor-porated into the PHB/PCL nanofibers. The structural, morphological, mechanical, wettability, solubility features and antibacterial activity of the CUR-CD-based PHB/PCL nanofibrous mats were investigated. In addition, the release studies of curcumin (CUR) from the PHB/PCL matrix were performed for each nanofiber composite. FE-SEM results displayed that the fiber diameter was the lowest for PHB/PCL/3%CUR-CD at 392.37 nm compared to the others. According to the results, we observed that adding CUR and CUR-CD improved the thermal stability of PHB/PCL nanofibers. The development of the complex between CUR and beta CD was supported by FT-IR analysis. Release test results revealed that the CUR-CD complex contributed to controlled release of CUR from fibers. The plate counting technique was used to examine the antibacterial activity nanofibrous mats. The antibacterial activity of PHB/PCL was significantly enhanced by incorporating the CUR-CD complex, particularly against Gram-positive Staphylococcus aureus. After 6 h of contact, the PHB/PCL/10%CUR-CD nanofiber mat exhibited a remarkable 97.79 % reduction in bacterial growth. The results demonstrated that the fabricated PHB/PCL/CUR-CD nanofibrous mats could be potential candidates for use as a wound dressing.Öğe Facile microwave-mediated green synthesis of non-toxic copper nanoparticles using Citrus sinensis aqueous fruit extract and their antibacterial potentials(Elsevier, 2021) Jahan, Israt; Erci, Fatih; Isildak, IbrahimThis study focuses on the utilization of orange (Citrus sinensis) fruit extract for microwave mediated green synthesis of copper nanoparticles for the first time. After completion of the synthesis cycles, the presence of a maximum peak at the wavelength of 560 nm by UV-vis spectroscopy indicated the formation of CuNPs. FTIR spectrum identified the functional biomolecules of the orange juice extract, responsible for the bioreduction, stabilization of copper nanoparticles. Scanning Electron Microscopy (SEM) indicated the presence of globular shaped nano-sized copper particles with smooth surfaces whereas Energy Dispersive X-ray Spectrometry (EDX) confirmed the presence of copper, carbon and oxygen atoms with the amount of 77.68%, 17.55% and 4.77%, respectively. Transmission Electron Microscopy (TEM) illustrated that the CuNPs are morphologically round in shape with the distribution range of 6.93 nm-20.70 nm in diameter. X-ray diffraction (XRD) analysis revealed the presence of face-centered cubic crystalline structures of copper nanoparticle, and Zeta potential analysis confirmed their high stability by showing the strong negative potential value (-25.60 mV). Moreover, these biosynthesized copper nanoparticles displayed their efficiency in controlling the growth of the bacteria, i.e., Escherichia coli and Staphylococcus aureus. The results revealed their non-cytotoxic nature on the L929 mouse fibroblast cell line through XTT cell viability assay. All these studies indicated that CuNPs synthesized by orange extract have the potential to serve as a green nanomaterial for biomedical applications.Öğe GREEN SYNTHESIS OF COPPER OXIDE NANOPARTICLES USING OCIMUM BASILICUM EXTRACT AND THEIR ANTIBACTERIAL ACTIVITY(Parlar Scientific Publications (P S P), 2017) Altikatoglu, Melda; Attar, Azade; Erci, Fatih; Cristache, Corina Marilena; Isildak, IbrahimThe investigation aims the synthesis of copper oxide nanoparticles (CuO NPs) using Ocimum basilicum plant extract at room temperature. This method is completely a green method, free from toxic and harmful solvent. The CuO NPs were sytnhesized by mixing copper sulphate dehydrate (CuSO4.5H20) and aqueous Ocimum basilicum leaf extract. The biosynthesized copper oxide nano-particles were characterized by UV-vis spectro-scopy, Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). The existence of the CuO NPs was revealed by UV-vis spectroscopy. The FTIR spectra of control (leaf extract) and synthesized CuO NPs identified the functional groups of the active components. SEM images brought out that the particles were spherical in shape, and the size was found to be ranging under 70 rim. Further, the synthesized CuO NPs were tested for antibacterial activity by standart disc diffusion method. The nanoparticles were found to have antibacterial activity against pathogenic bacterial strains Escherichia coli and Staphylococcus aureus by the observation of inhibition zones around each well.Öğe Green synthesis of silver nanoparticles using Thymbra spicata L. var. spicata (zahter) aqueous leaf extract and evaluation of their morphology-dependent antibacterial and cytotoxic activity(Taylor & Francis Ltd, 2018) Erci, Fatih; Cakir-Koc, Rabia; Isildak, IbrahimSilver (Ag) nanoparticles (NPs) were green synthesized at room temperature using different concentrations of the Thymbra spicata L. var. spicata (Zahter) aqueous leaf extracts for the first time. With the synthesis of AgNPs using the leaf extract of Cynara scolymus (Artichoke) and Mentha piperita (Peppermint), the biological activities of the nanoparticles synthesized using leaf extract of three economically significant plants have been studied comparatively. Nanoparticles were characterized by different spectroscopic and microscopic analysis. TEM analysis of the biosynthesized AgNPs revealed that the size and shape of the AgNPs were changed with the plant extract concentration. Biologically synthesized AgNPs from leaf extracts of the three different plants displayed significant differences in antibacterial activity against two different gram-negative and gram-positive bacteria. Also, the results from this study show the shape dependence of the antibacterial and cytotoxic activity of silver nanoparticles synthesized using T. spicata leaf extract. The nanoparticles with different shapes exhibited the strongest antibacterial and cytotoxic activity compared to mostly spherical nanoparticles. Present results clearly indicate that biological activities of silver nanoparticles were affected by nanoparticle shape and the source of the plant extract used in the synthesis.Öğe Microwave-Assisted Green Synthesis of Non-Cytotoxic Silver Nanoparticles Using the Aqueous Extract of Rosa santana (rose) Petals and Their Antimicrobial Activity(Taylor & Francis Inc, 2019) Jahan, Israt; Erci, Fatih; Isildak, IbrahimGreen methods using biological extracts, in particular plant-based solutions, have shown great potential for silver nanoparticle synthesis. A microwave-assisted single-step phytosynthesis of silver nanoparticles is described in the present study. The aqueous extract obtained from the Rosa santana (rose) petals was used for the first time in the synthesis. The synthesized nanoparticles obtained after optimized microwave conditions for time and temperature were analyzed by ultraviolet-visible spectroscopy (UV-Vis), Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and Zeta-size analysis. The results obtained from the characterization studies showed that the synthesized nanoparticles were nearly spherical in shape with sizes from 6.52 nm to 25.24 nm with an average particle size of 14.48 nm with a face-centered cubic structure. The antibacterial activities of the synthesized nanoparticles were evaluated and revealed that the silver nanoparticles displayed good inhibition against both Gram-negative and Gram-positive bacteria. Also, the cytotoxic effect of the silver nanoparticles on a mouse fibroblast cell line (L929) was studied by a cell viability assay. The results showed that phytosynthesized silver nanoparticles were nontoxic to the healthy normal cell line at all tested concentrations.Öğe Microwave-irradiated green synthesis of metallic silver and copper nanoparticles using fresh ginger (Zingiber officinale) rhizome extract and evaluation of their antibacterial potentials and cytotoxicity(Taylor & Francis Inc, 2021) Jahan, Israt; Erci, Fatih; Cakir-Koc, Rabia; Isildak, IbrahimHerein the microwave-assisted green synthesis of both silver (Ag) and copper (Cu) nanoparticles (NPs) by microwave irradiation have been successfully carried out using fresh ginger rhizome extract. Different analytical methods were used for the characterization of the nanoparticles. The size distribution of AgNPs was found from the range of 2.68 nm to 42.69 nm, whereas for CuNPs the size range was run from 11.32 nm to 33.70 nm in diameter. From the antibacterial assay, we found that both silver and copper nanoparticles demonstrated significant potentials against tested bacterial strains. Besides,in vitrocytotoxic effects of NPs were also tested on the L929 mouse fibroblast cell line through XTT cell viability assay which confirmed the non-toxic nature of AgNPs and CuNPs at all tested concentrations. Thus, the obtained biological properties, suggested the use of rhizome extract ofZingiber officinalemediated non-toxic AgNPs and CuNPs in further biomedical and clinical applications.Öğe Plant extract mediated silver nanoparticles by concentrated sunlight and their antibacterial and cytotoxic activities(Taylor & Francis Inc, 2022) Kutuk, Yagmur; Yontem, Mustafa; Erci, Fatih; Esirgenler, Busra; Isildak, Ibrahim; Totu, Eugenia EftimieHere we report the synthesis of silver nanoparticles (AgNPs) under concentrated sunlight by using a newly designed solar system. In the study, AgNPs synthesized by using different plant extracts of Ginko biloba (Gb), Alpinia officinarum (Ao), Foeniculum vulgare (Fv) and Cassia angustifolia (Ca) were characterized by different techniques. The antibacterial activity of the AgNPs was determined by agar diffusion assay. TEM analysis revealed the spherical nanoparticles with average size of 26, 23,44 and 32 nm in diameter for Gb, Ao, Fv and Ca AgNPs, respectively. Also, we found that the AgNPs were not cytotoxic at the concentration of 2.5 mu g/mL. We also revealed that Ca AgNPs showed the highest antibacterial activity against S. aureus with inhibition zone of 15.27 mm. All in all, the results indicated that the formation of AgNPs was achieved in less than 1 h without any energy consumption in the solar system with concentrated sunlight.Öğe Production of alginate films containing Hypericum perforatum extract as an antibacterial and antioxidant wound dressing material(Sage Publications Ltd, 2022) Mutlu, Betul; Erci, Fatih; Koc, Rabia CakirThe incorporation of herbal extracts in wound dressing materials is an important concept that has been researched recently. In this study, alginate films incorporated in the various ratio (0.25-1% v/v) of Hypericum perforatum extract (HPE) for potential applications of wound dressing were successfully prepared by solvent-casting method. The obtained films were examined for cytotoxicity, in vitro wound healing potential, swelling behavior, antioxidant, and antibacterial properties. When compared to the alginate film (Al) alone, HPE incorporated alginate films (HPE/Al) exhibit improved antioxidant properties according to the results of CUPRAC assay and antioxidant activity increases with the rate of HPE. Also, HPE/Al films exhibited antibacterial activity against E. coli and S. aureus, and addition with the HPE extract into films significantly increased the antibacterial activity against S. aureus. All film samples had no cytotoxic effects on fibroblast cell line and HPE/Al films showed a proliferative effect with high extract concentrations (1%) compared to extract free-films. Also, scratch assay results show that films containing 0.5% (v/v) HPE may have a positive effect on wound healing. The results have shown that the newly developed HPE incorporated alginate films are a candidate as antibacterial, and antioxidant wound dressing for use on burn or excision wounds.Öğe Rapid green synthesis of non-cytotoxic silver nanoparticles using aqueous extracts of 'Golden Delicious' apple pulp and cumin seeds with antibacterial and antioxidant activity(Springer Int Publ Ag, 2021) Jahan, Israt; Erci, Fatih; Isildak, IbrahimA simple, facile and rapid microwave irradiated system was applied to synthesize silver nanoparticles using 'Golden Delicious' apple pulp (Malus domestica) and cumin (Cuminum cyminum) seed extracts. The phytosynthesized AgNPs were characterized by Ultraviolet-Visible Spectroscopy (UV-vis), Fourier transform infrared (FTIR), X-ray Diffraction (XRD) Transmission Electron Microscopy (TEM) and Zeta sizer analysis. In the study, the presence of face-centered cubic crystalline structured metallic silver in AgNPs from apple and cumin extracts and the monodisperse nature of AgNPs with the size distribution range of 5.46-20 nm and 1.84-20.57 nm were confirmed, respectively. This study established an efficient green synthesis approach that created so far, the smallest silver nanoparticles by using these two extracts. According to the results obtained, AgNPs synthesized using both extracts were non-toxic against L929 mouse fibroblast cells, while they were effective against both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria with a greater effect on S. aureus. Moreover, AgNPs synthesized through cumin extract exhibited a higher ABTS scavenging ability (96.43 +/- 0.78% at 160 mu g/mL) in comparison to apple pulp extract mediated AgNPs, while both AgNPs showed lower activity for DPPH (27.84 +/- 0.56% and 13.12 +/- 0.32% from cumin seed and apple pulp extracts, respectively). In summary, our results suggest the green non-cytotoxic AgNPs synthesized in this study could be a promising template for further biological and clinical applications. [GRAPHICS]Öğe Rapid green synthesis of noncytotoxic iron oxide nanoparticles using aqueous leaf extract ofThymbra spicataand evaluation of their antibacterial, antibiofilm, and antioxidant activity(Taylor & Francis Inc, 2021) Erci, Fatih; Cakir-Koc, RabiaHerein the green synthesis of iron oxide (FeO) nanoparticles (NPs) was achieved by usingThymbra spicataleaf extract. The antibacterial activity of FeONPs was evaluated against both gram-positive and gram-negative bacteria by using broth microdilution assay. Further, the antioxidant and cytotoxic potential of FeONPs was determined by using standard protocols. The effects of nanoparticles onStaphylococcus aureusgrowth and on their biofilm formation were investigated. FeONPs at the concentration of 100 mu g/mL exhibited excellent bactericidal action againstSalmonella typhimurium. Also, the biogenic nanoparticles inhibited the growth rate ofS. aureus.Furthermore, FeONPs showed noncytotoxic characteristics on L929 mouse fibroblast cells. All these results revealed that FeONPs obtained by simple and eco-friendly synthesis process throughT. spicataextract could be considered a promising candidate as antibacterial agent for many different applications. Also, biogenic FeONPs with their antibiofilm activity onS. aureuscould serve as potent antibacterial agents against pathogenic biofilm-forming bacteria.Öğe Synthesis and Antimicrobial Activity of Some 2-aminothiazole and 2-aminothiadiazine Derivatives(Wiley, 2018) Sariguney, Ahmet Burak; Kocabas, Erdal; Erci, Fatih; Torlak, Emrah; Coskun, AhmetIn this study, some thiazole and thiadiazine ring bearing compounds were synthesized, characterized by spectroscopic techniques, and evaluated as potential antimicrobial agents. Their antimicrobial activities evaluated by broth microdilution method and expressed as minimum inhibitory concentration; against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus. From these compounds, Compounds 2, 5, and 9 have been found to selectively inhibit Gram positives.Öğe Synthesis of biologically active copper oxide nanoparticles as promising novel antibacterial-antibiofilm agents(Taylor & Francis Inc, 2020) Erci, Fatih; Cakir-Koc, Rabia; Yontem, Mustafa; Torlak, EmrahIn this study, we aimed to synthesize copper oxide nanoparticles (CuONPs) mediated by plant extract in an environmentally friendly way and to reveal their potential biological activities. Here we synthesized CuONPs by using different concentrations of aqueous leaf extract of Thymbra spicata at 80 degrees C to obtain Ts1CuONPs and Ts2CuONPs. Biosynthesized nanoparticles were characterized by using UV-Vis, AFM, FTIR, SEM-EDS, TEM, DLS and zeta potential analysis. The antibacterial activity of the nanoparticles was determined by calculation of the inhibition zone and minimum inhibitory concentration against selected bacterial strains. Moreover, the antioxidant activity of the as-synthesized nanoparticles was evaluated based on DPPH radical scavenging activity. The results indicate that the as-synthesized NPs have an average size of 26.8 and 21 nm for Ts1CuONPs and Ts2CuONPs, respectively. The formed CuONPs have more antibacterial action on gram-positive bacteria compared to gram-negative bacteria. In addition, CuONPs demonstrated good inhibition activity against biofilm formation of Staphylococcus aureus (S. aureus). Furthermore, the results showed that the smaller size of the CuONPs caused the higher cytotoxicity on L929 mouse fibroblast cells. The as-synthesized CuONPs exhibit antibacterial and antibiofilm potential against S. aureus, indicating that they may be attractive candidates to use in future therapeutic applications.
