Antimicrobial and antibiofilm activity of green synthesized silver nanoparticles by using aqueous leaf extract of Thymus serpyllum
Yükleniyor...
Tarih
2019
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Recently, metal nanoparticles have attracted the attention of researchers due to their unique properties whencompared with bulk materials and have become used in many fields of application. In this study, green synthesis ofAg nanoparticles (AgNPs) was investigated by using the aqueous extract of T. serpyllum leaves. In addition,antimicrobial and antibiofilm activities of the synthesized AgNPs were evaluated in this study. Further, ultraviolet-visible spectroscopy (UV-Vis), fouirer transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) and transmission electron microscopy (TEM) were used for characterization of the green synthesized AgNPs. The UV-Vis spectrum of the synthesizedAgNPs had a maximum peak at 467 nm. Also, TEM analysis indicated spherical particles with an average size of 25.2 nm. The synthesized AgNPs have higher stability (zeta potential: -29.5 mV). The antimicrobial activity of thegreen synthesized AgNPs was investigated on both Gram-positive and Gram-negative bacteria, such as Bacilluscereus (B. cereus), Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Salmonella enterica serovarTyphimurium (S. Typhimurium) using agar well diffusion assay. According to the results of the study, Gram-positivebacteria showed larger inhibition zones compared to Gram-negative bacteria. Finally, the AgNPs were explored forthe inhibition of S. aureus biofilms. AgNPs at 100 ?g/mL concentration showed a high inhibition value of about 73%for S. aureus biofilm formation. So, it is concluded that the synthesized AgNPs might be potentially used in manyapplications due to their antimicrobial and antibiofilm properties.
Açıklama
Anahtar Kelimeler
Green synthesis, Silver nanoparticle, Antimicrobial, Antibiofilm
Kaynak
Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi
WoS Q DeÄŸeri
Scopus Q DeÄŸeri
Cilt
23
Sayı
3
Künye
Erci, F., Torlak, E. (2019). Antimicrobial and antibiofilm activity of green synthesized silver nanoparticles by using aqueous leaf extract of Thymus serpyllum. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 23, 3, 333-339.
