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Öğe Silver nanoparticle doped graphene-based impedimetric biosensor towards sensitive detection of procalcitonin(Elsevier, 2023) Selimoğlu, Faysal; Gür, Bahri; Ayhan, Muhammed Emre; Gür, Fatma; Kalita, Golap; Tanemura, Masaki; Alma, Mehmet HakkıIn the early detection of sepsis, procalcitonin (PCT) appears to be a highly sensitive biomarker for severe inflammation and infection. A significant interaction between the electrode material to be used in the design of the biosensor and the material to be attached to the surface is of great importance. Here, we demonstrated a silver nanoparticle (AgNp) doped graphene-based sensitive PCT biosensor with low-cost, environmentally friendly materials. Cyclic voltammetry curves showing the reusability of the electrodes obtained were obtained and showed antibody-protein adhesion on the AgNp/SLG@ITO surface. The anodic and cathodic peak currents values after 20 cycles show that these values are suitable even after 20 measurements. Electrochemical impedance spectroscopy (EIS) studies confirmed the effects of PCT on binding events due to increasing con-centration at a constant PCT-antibody concentration. The limit of detection (LOD) value of the fabricated PCT/ Ab/AgNp/SLG@ITO impedimetric biosensor was determined as 0.55 ngmL-1. The low LOD value can be attributed to the uniform and large surface area of single-layer graphene (SLG) and noble AgNp. The LOD value based on the EIS studies has revealed that the PCT/Ab/AgNp/SLG@ITO impedimetric biosensor can be employed in real samples.Öğe Karyological study on endemic Sonchus erzincanicus Matthew (Asteraceae) in Turkey(2013) Altınordu, Fahim; Kandemir, Ali; Martin, EsraSonchus erzincanicus Matthews (Asteraceae) Türkiye için endemiktir ve doğal olarak Erzincan’da yayılış gösterir. Bu çalışmada, Erzincan’dan toplanan Sonchus erzincanicus türünün somatik kromozom sayısı belirlendi ve karyotip analizi yapıldı. Türün kromozom sayısı 2n18 olarak tespit edildi. Bu türün Görüntü Analiz Sistemi aracılığı ile Karyotip Analizi yapıldı. Haploit kromozom uzunluğu 20.11 ?m’dir ve en küçük kromozom 1.63 ?m en büyük kromozom ise 2.97 ?m uzunluğundadır. Türün karyotip formülü altı median ve üç submedian kromozom tiplerinden oluşmaktadır. Bu türün karyotip analizi ilk defa çalışmamızda analiz edildi.Öğe Antimicrobial and antibiofilm activity of green synthesized silver nanoparticles by using aqueous leaf extract of Thymus serpyllum(2019) Erci, Fatih; Torlak, EmrahRecently, metal nanoparticles have attracted the attention of researchers due to their unique properties whencompared with bulk materials and have become used in many fields of application. In this study, green synthesis ofAg nanoparticles (AgNPs) was investigated by using the aqueous extract of T. serpyllum leaves. In addition,antimicrobial and antibiofilm activities of the synthesized AgNPs were evaluated in this study. Further, ultraviolet-visible spectroscopy (UV-Vis), fouirer transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) and transmission electron microscopy (TEM) were used for characterization of the green synthesized AgNPs. The UV-Vis spectrum of the synthesizedAgNPs had a maximum peak at 467 nm. Also, TEM analysis indicated spherical particles with an average size of 25.2 nm. The synthesized AgNPs have higher stability (zeta potential: -29.5 mV). The antimicrobial activity of thegreen synthesized AgNPs was investigated on both Gram-positive and Gram-negative bacteria, such as Bacilluscereus (B. cereus), Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Salmonella enterica serovarTyphimurium (S. Typhimurium) using agar well diffusion assay. According to the results of the study, Gram-positivebacteria showed larger inhibition zones compared to Gram-negative bacteria. Finally, the AgNPs were explored forthe inhibition of S. aureus biofilms. AgNPs at 100 ?g/mL concentration showed a high inhibition value of about 73%for S. aureus biofilm formation. So, it is concluded that the synthesized AgNPs might be potentially used in manyapplications due to their antimicrobial and antibiofilm properties.Öğe Essential oil characterization of Cousinia sivasica Hub.-Mor. (Asteraceae)(2018) Tekin, Mehmet; Özek, Gülmira; Martin, Esra; Özek, Temel; Yılmaz, Gülden; Başer, Kemal Hüsnü CanIn this study, essential oil properties of an endemic plant of Turkey, Coucinia sivasica Hub.-Mor. were studiedfor the first time. Essential oil of C. sivasica was obtained by using Clevenger apparatus with hydrodistillationtechnique. The essential oil was analyzed by GC-FID and GC/MS techniques on a polar column and total 44compounds were identified representing 98.3. Fatty acids, their esters, methyl-branched carboxylic acids and aromaticacid esters predominated (66.3) in the oil with hexadecanoic acid (42.8), 1-isobutyl 4-isopropyl 3-isopropyl-2,2-dimethyl succinate (12.2) and methyl salicylate (7.1) as major constituents.Öğe High-Throughput Genomic Simple Sequence Repeat (SSR) Marker Development and Construction of a High Resolution Physical Map in Button Mushroom (Agaricus bisporus) Genome(2018) Uncu, Ali Tevfik; Özgür Uncu, AyşeButton mushroom [Agaricus bisporus (J.E. Lange) Imbach], is both a model organism and a worldwide cultivated edible mushroom species. Despite its scientific and economic relevance, efforts to develop molecular markers in A. bisporus genome are scarce and relatively recent. The present research reports the development of 3943 novel simple sequence repeat (SSR) markers specific to the A. bisporus genome. The marker set is well-distributed among the 13 A. bisporus chromosomes, saturating the entire genome with sequence-specific markers. The large number of novel, A. bisporus specific DNA markers introduced in the present work constitute a valuable resource for molecular genetic research in button mushroom, including strain identification and protection, and gene/QTL (Quantitative Trait Locus) mapping.Öğe Effect of Tween 80 on Conjugated Linoleic Acid Productionby Lactobacillus Strains in Reconstituted Skim Milk Powder(2016) Torlak, Emrah; Yalçın, Suzan; Erci, FatihBu çalışmada üç adet konjuge linoleik asit (KLA) üreten Lactobacillus suşu 2000 µg/ml linoleik asit (LA) ve çeşitli konsantrasyonlarda Tween 80 ilave edilmiş rekonstitüe yağsız süt tozu (%10) içinde 36 saate kadar kültüre edilmiştir. İnkübasyon boyunca kültür süpernatantlarında toplam KLA düzeyleri UV-spektrofotometre ile tespit edilmiştir. KLA düzeyleri 5 ve 20 mg/ml Tween 80 ilavesi ile önemli (P0.05) seviyede artmıştır. Bununla birlikte, Tween 80 konsantrasyonunun 20 mg/ml'den 40 mg/ml'ye arttırılması KLA düzeyinde bir artışa neden olmamıştır. İnkübasyon esnasında Lactobacillus suşlarının gelişme hızı ve KLA üretimlerinde benzer artış oranları gözlenmiştir.Öğe Obez Kadınlarda Metabolik Sendrom ve Lipid Profilinin Değerlendirilmesi(2015) Korkut, Yasemin; Koçak, Fatma Emel; Paşalı Kilit, Türkan; Arıkan, İnci; Tekşen, Yasemin; Yöntem, Mustafa; Birgül, MehmetAmaç: Çalışmada obez kadınlarda Metabolik Sendrom (MS) ve kan lipid profilinin değerlendirilmesi amaçlandı. Yöntem: Aralık 2013-Ocak 2014 tarihleri arasında polikliniğe kilo verememe nedeniyle başvuran 102 hastada, MS ve kan parametreleri değerlendirildi. VKİ; 30,0-34,9 kg/m2 ise 1. derece obez, 35,0-39,9kg/m2 ise 2. derece obez, 40,0kg/m2 ise 3. derece (morbid) obez olarak sınıflandırıldı. Bulgular: Hastaların yaş ortalaması; 45,0111,0 idi. Kadınların %34,3ü 1. derece, %30,4ü 2. derece, %35,3ü 3. derece obez idi. MS prevalansı %56,9 olarak bulundu. Obezite derecelerine göre MS sıklığı artarken, yaş arttıkça MSnin arttığı saptandı (p0,05). Hastaların, obezite derecelerine göre bazı kan parametreleri değerlendirildiğinde; obezite derecesi arttıkça kan glukoz düzeyi ortalamalarının (p0,048), HOMA-IR (p0,009) ve insülin düzeyinin (p0,031) anlamlı bir şekilde yükseldiği bulunmuş olmasına karşın; obezite derecesi arttıkça TG, Total kolesterol, LDL-kolesterol ve HbA1c düzeyleri istatistiksel olarak anlamlı olmasa da artış olduğu gösterildi (p0,05). Sonuç: Çalışmamızda, VKİ artışı ile MSnin arttığı ve lipid profilindeki bozulma ile VKİ arasında anlamlı bir ilişki bulunmasa da, doğru orantının olduğu görülmektedir. Bunun yanı sıra VKİ artışı ile orantılı olarak kan glukozu, HOMA-IR, insülin düzeylerinde anlamlı bir artış tespit edilmiş olup beslenme ve yaşam tarzı değişiklikleri ile sadece kadınların değil, tüm ailenin bu durumdan olumlu yönde etkileneceği ve obezite, dislipidemi ve sonuç olarak MSye gidişte azalma olacağı açıktır.Öğe Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica(2016) Çakmak, Derya; Karaoğlu, Cuma; Aasım, Muhammad; Sancak, Cengiz; Özcan, Sebahatint: The present study addressed the successful in vitro seed germination, bulblet regeneration, increased bulblet size, hardening, and acclimatization of Persian lily (Fritillaria persica). Seed germination rate was recorded as 86.7% and 96.7% after 2 and 3 months of cold treatment at 4 °C, respectively. Bulblet explants taken from a germination experiment were cultured on Murashige and Skoog (MS) medium supplemented with 0.5–2.0 mg/L thidiazuron (TDZ). Maximum bulblet regeneration frequency and bulblets per explants were achieved on a medium containing 2.0 mg/L TDZ. Leaf disc and leaf scale explants isolated from germinated seedlings were also cultured on MS medium containing 2–6 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) with a constant concentration of 0.2 mg/L kinetin. Both explants yielded the highest bulblet regeneration on MS medium containing 2 mg/L 2,4-D and 0.2 mg/L kinetin. A combination of 1.5 g/L agar and 1.6 g/L GELRITE resulted in a maximum bulblet diameter with a higher number of roots. The addition of 10 g/L NaCl to the culture medium resulted in the best root and leaf formation and secondary bulblet regeneration. Exposure of regenerated bulblets to 10 g/L NaCl at 4 °C for 2 months on a medium with 20 g/L sucrose was also found to be beneficial for acclimatization.Öğe A new species and a new natural hybrid of Origanum L. (Lamiaceae) from the west of Turkey(2018) Dirmenci, Tuncay; Yazıcı, Türker; Özcan, Taner; Çelenk, Sevcan; Martin, EsraOriganum ayliniae Dirmenci & Yazıcı, which differs significantly from O. dictamnus L., is described for the first time in this paper. In addition, a new hybrid, O. adae Dirmenci & Yazıcı, between O. ayliniae and O. sipyleum L., is described and investigated in detail. The general morphology, pollen structure, chromosome features, and nuclear (ITS) and chloroplast (rpl32) genomes of collected specimens were studied. O. adae was determined to have some intermediate properties morphologically and palynologically that place it between the parents. Pollen size and shape were important features in the diagnosis of the hybrid and its parents in this study. The somatic chromosome number of O. ayliniae, O. adae, and O. sipyleum was counted as 2n = 30. According to the results of these different approaches, O. ayliniae differs significantly from O. dictamnus, which has a close relationship with O. ayliniae, and O. adae has a hybrid nature. These results support previous reports for the speciation of Origanum members via hybridization. This study aimed to present a new species (O. ayliniae), a new hybrid of O. adae, and their differences. In conclusion, O. ayliniae, O. adae, and O. sipyleum provide significant morphological, micromorphological, palynological, cytological, and molecular information about homoploid hybridization.Öğe Study of phylogenetic relationship of Turkish species ofMatthiola (Brassicaceae) based on ISSR amplification(2016) Doğan, Bekir; Çelik, Mustafa Bahattin; Ünal, Murat; Sefalı, Abdurrahman; Martin, Esra; Kaya, AylaMatthiola W.T.Aiton is a taxonomically complex genus in which there are many problems, mostly with Matthiola longipetalaand M. odoratissima. Matthiola species native to Turkey were collected from various locations in Anatolia, and their DNA was isolated. Revision studies performed on the basis of molecular data obtained from studies conducted in recent years have made the phylogenetic relationships and systematic positions of the taxa more apparent and reliable. Consequently, the remaining taxonomic problems among the species have been resolved through the use of DNA-based molecular analysis methods, which, unlike phenotype studies, are not affected by environmental factors. The inter-simple sequence repeat (ISSR) fingerprinting method was used in the study because its properties were considered to be more reliable and consistent than those of the randomly amplified polymorphic DNA method. DNA fragments were amplified through the use of ISSR primers. The phylogenetic relationships among the taxa were represented on a dendrogram constructed through means of NTSYSpc 2.02 software. The infrageneric and intergeneric phylogenetic relationships between Matthiola and other related genera were also characterized. It was determined that the taxa Matthiola odoratissima and M. ovatifolia are separate but closely related. Moreover, it was observed that the Matthiola longipetala complex forms a separate group within the genera. Clearly, the genera Matthiola, Sterigmostemum, Strigosella, Malcolmia, and Chorispora are phylogenetically differentiated on the dendrogram.Öğe Cytogenetic studies on some Scorzonera L. s.l. (Asteraceae) taxa from Turkey(2015) Martin, Esra; Altınordu, Fahim; Makbul, Serdar; Çoşkunçelebi, Kamil; Gültepe, MutluIn the present study, chromosome morphology of 32 Scorzonera L. taxa, including 19 species endemic to Turkey, was analyzed. Te plant materials were collected from diferent natural populations of Turkey between 2005 and 2011. Te classification of chromosomes, the length of the long and short arm, haploid chromosome length, arm ratio, and relative chromosomal length were measured by sofware image analyses. Te basic chromosome numbers were determined as x 6 and x 7. Two types of ploidy levels were observed as diploidy and tetraploidy. Karyotype asymmetry indices, TF%, As K%, Syi, Rec, A, A1, and A2 were also calculated. Scorzonera ahmet-duranii S.Makbul and Coskuncelebi, S. laciniata subsp. calcitrapifolia (Vahl) Marie, and Scorzonera suberosa C.Koch subsp . cariensis (Boiss.) Chamberlain had the most symmetrical karyotypes for the subgenera Scorzonera L., Podospermum (L.) DC., and Pseudopodospermum (Lipsch. and Krasch.) Lipsch., respectively.Öğe Bellevalia vuralii B.Şahin & Aslan (Asparagaceae): a new species from SE Turkey(2016) Martin, Esra; Şahin, Bilal; Aslan, Serdar; Karabacak, OsmanBellevalia vuralii B.Şahin & Aslan is described as a new species of Bellevalia from southeast Turkey. As well as its morphological characteristics and classification with regards to sections, its description, ecology, and relationship with related species are presented and discussed. The somatic chromosome number of these species was defined to be 2n = 8.
