Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica
Yükleniyor...
Dosyalar
Tarih
2016
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
t: The present study addressed the successful in vitro seed germination, bulblet regeneration, increased bulblet size, hardening,
and acclimatization of Persian lily (Fritillaria persica). Seed germination rate was recorded as 86.7% and 96.7% after 2 and 3 months of
cold treatment at 4 °C, respectively. Bulblet explants taken from a germination experiment were cultured on Murashige and Skoog (MS)
medium supplemented with 0.5–2.0 mg/L thidiazuron (TDZ). Maximum bulblet regeneration frequency and bulblets per explants were
achieved on a medium containing 2.0 mg/L TDZ. Leaf disc and leaf scale explants isolated from germinated seedlings were also cultured
on MS medium containing 2–6 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) with a constant concentration of 0.2 mg/L kinetin. Both
explants yielded the highest bulblet regeneration on MS medium containing 2 mg/L 2,4-D and 0.2 mg/L kinetin. A combination of 1.5
g/L agar and 1.6 g/L GELRITE resulted in a maximum bulblet diameter with a higher number of roots. The addition of 10 g/L NaCl to
the culture medium resulted in the best root and leaf formation and secondary bulblet regeneration. Exposure of regenerated bulblets to
10 g/L NaCl at 4 °C for 2 months on a medium with 20 g/L sucrose was also found to be beneficial for acclimatization.
Açıklama
WOS:000378689600017
Anahtar Kelimeler
Fritillaria persica, Micropropagation, Secondary bulblets, Bulblet size, Hardening
Kaynak
Turkish Journal of Biology
WoS Q Değeri
Q3
Scopus Q Değeri
Q2
Cilt
40
Sayı
4
Künye
Aasim, M., Çakmak, D., Karaoğlu, C., Sancak, C., Özcan, S. (2016). Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica. Turkish Journal of Biology, 40, 4, 878-888.
