Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica

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dc.authoridMuhammad Aasım: 0000-0002-8524-9029en_US
dc.contributor.authorÇakmak, Derya
dc.contributor.authorKaraoğlu, Cuma
dc.contributor.authorAasım, Muhammad
dc.contributor.authorSancak, Cengiz
dc.contributor.authorÖzcan, Sebahatin
dc.date.accessioned2020-01-18T21:03:02Z
dc.date.available2020-01-18T21:03:02Z
dc.date.issued2016
dc.departmentNEÜ, Fen Bilimleri Fakültesi, Biyoteknoloji Anabilim Dalıen_US
dc.descriptionWOS:000378689600017en_US
dc.description.abstractt: The present study addressed the successful in vitro seed germination, bulblet regeneration, increased bulblet size, hardening, and acclimatization of Persian lily (Fritillaria persica). Seed germination rate was recorded as 86.7% and 96.7% after 2 and 3 months of cold treatment at 4 °C, respectively. Bulblet explants taken from a germination experiment were cultured on Murashige and Skoog (MS) medium supplemented with 0.5–2.0 mg/L thidiazuron (TDZ). Maximum bulblet regeneration frequency and bulblets per explants were achieved on a medium containing 2.0 mg/L TDZ. Leaf disc and leaf scale explants isolated from germinated seedlings were also cultured on MS medium containing 2–6 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) with a constant concentration of 0.2 mg/L kinetin. Both explants yielded the highest bulblet regeneration on MS medium containing 2 mg/L 2,4-D and 0.2 mg/L kinetin. A combination of 1.5 g/L agar and 1.6 g/L GELRITE resulted in a maximum bulblet diameter with a higher number of roots. The addition of 10 g/L NaCl to the culture medium resulted in the best root and leaf formation and secondary bulblet regeneration. Exposure of regenerated bulblets to 10 g/L NaCl at 4 °C for 2 months on a medium with 20 g/L sucrose was also found to be beneficial for acclimatization.en_US
dc.identifier.citationAasim, M., Çakmak, D., Karaoğlu, C., Sancak, C., Özcan, S. (2016). Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica. Turkish Journal of Biology, 40, 4, 878-888.en_US
dc.identifier.doi10.3906/biy-1510-18en_US
dc.identifier.endpage888en_US
dc.identifier.issn1300-0152en_US
dc.identifier.issn1303-6092en_US
dc.identifier.issue4en_US
dc.identifier.scopusqualityQ2en_US
dc.identifier.startpage878en_US
dc.identifier.urihttps://app.trdizin.gov.tr/makale/TWpFME56UXhNUT09/advancement-in-protocol-for-in-vitro-seed-germination-regeneration-bulblet-maturation-and-acclimatization-of-fritillaria-persica
dc.identifier.urihttps://hdl.handle.net/20.500.12452/1421
dc.identifier.volume40en_US
dc.identifier.wosWOS:000378689600017en_US
dc.identifier.wosqualityQ3en_US
dc.indekslendigikaynakWeb of Scienceen_US
dc.indekslendigikaynakScopusen_US
dc.indekslendigikaynakTR-Dizinen_US
dc.language.isoenen_US
dc.relation.ispartofTurkish Journal of Biologyen_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US]
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFritillaria persicaen_US
dc.subjectMicropropagationen_US
dc.subjectSecondary bulbletsen_US
dc.subjectBulblet sizeen_US
dc.subjectHardeningen_US
dc.titleAdvancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persicaen_US
dc.typeArticleen_US

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