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Öğe Carbon quantum dot modified electrodes developed for electrochemical monitoring of Daunorubicin-DNA interaction(Elsevier Science Sa, 2020) Eksin, Ece; Senturk, Huseyin; Zor, Erhan; Bingol, Haluk; Erdem, ArzumThe carbon quantum dot (cQD) modified disposable pencil graphite electrodes were developed for the first time in this study for electrochemical monitoring of drug-DNA interaction. The biomolecular interaction between calf thymus double stranded DNA (ctDNA) and an anthracycline antineoplastic drug, Daunorubicin (DNR) was investigated by differential pulse voltammetry (DPV) technique. For this purpose, experimental conditions, such as, the concentration of ctDNA, concentration of DNR and interaction time were optimized. Under optimum conditions, the detection limits of DNR and ctDNA were found to be 0.02 mu g/mL and 0.89 mu g/mL, respectively. The effect of interaction time between DNR and ctDNA was explored upon to the changes at both DNR and guanine oxidation signals. Drug-DNA interaction process was also examined by electrochemical impedance spectroscopy (EIS) technique.Öğe Carboxylated-Graphene Decorated Pencil Graphite Electrode as a Platform for Voltammetric Detection of DNA(Electrochemical Soc Inc, 2017) Zor, Erhan; Eksin, Ece; Findik, Mukerrem; Bingol, Haluk; Erdem, ArzumContinuous technology miniaturization using nanoscale materials allows the design of DNA sensing platforms with not only far larger surface areas but alsomore effective characteristics. We herein synthesized benzoic acid-functionalized graphene oxide (GOB), characterized by FT-IR, XPS, Raman Spectroscopy, TGA, SEM, TEM techniques and then it was utilized as a supporting material. Due to the benzoic acid groups available on the surface of GOB, fsDNA was directly immobilized onto the disposable pencil graphite electrode (PGE). The limit of detection (LOD) for fish sperm DNA (fsDNA) concentration was estimated to be 1.11 mu g/mL by using differential pulse voltammetry (DPV) technique. It can be assumed that this simple modification protocol offers an alternative for the quick and robust monitoring of sequence-specific nucleic acids avoiding the need of complex or expensive requirements, and it can pave the way for new opportunities to design more novel DNA sensing strategies and applications on disposable platforms. (c) 2017 The Electrochemical Society. All rights reserved.Öğe Electrochemical detection of interaction between daunorubicin and DNA by hybrid nanoflowers modified graphite electrodes(Elsevier Science Sa, 2021) Findik, Mukerrem; Bingol, Haluk; Erdem, ArzumIn this study, two types of hybrid nanoflowers (NFs) modified disposable electrodes were developed to investigate the electrochemical behavior of anticancer drug-DNA interaction. Biocompatible hybrid NFs were synthesized by amino acids (L-glutamic acid and L-cysteine) via a simple, rapid and cost-effective methods. Structural characterizations of the synthesized hybrid NFs were performed with Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS), while morphological characterizations were performed using scanning electron microscopy (SEM) and energy diffuser X-ray (EDX) spectroscopy techniques. As the sensing platform in electrochemical studies, pencil graphite electrode (PGE) has been used because of its advantages such as low cost, easy-to-handle, portable and disposable. After PGEs modified with NFs were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques, differential pulse voltammetry (DPV) technique was used in the determination of calf thymus double stranded DNA (ctdsDNA) and also daunorubicin (DNR) as well as DNA-DNR interaction. The detection limits as a result of ctdsDNA determination were found to be 0.93 mu g/mL by using Lcysteine NFs modified PGE (c-NFs-PGE) and 2.37 mu g/mL by using L-glutamic acid modified PGE (ga-NFs-PGE). The detection limits obtained for the determination of DNR were found as 2.93 mu M and 2.06 mu M with c-NFs-PGE and ga-NFs-PGE, respectively. The optimum interaction time between DNR and ctdsDNA was determined by cNFs-PGE, which showed higher sensitivity performance against oxidation of guanine signals.Öğe Electrochemical monitoring of biointeraction by graphene-based material modified pencil graphite electrode(Elsevier Advanced Technology, 2017) Eksin, Ece; Zor, Erhan; Erdem, Arzum; Bingol, HalukRecently, the low-cost effective biosensing systems based on advanced nanomaterials have received a key attention for development of novel assays for rapid and sequence-specific nucleic acid detection. The electrochemical biosensor based on reduced graphene oxide (rGO) modified disposable pencil graphite electrodes (PGEs) were developed herein for electrochemical monitoring of DNA, and also for monitoring of biointeraction occurred between anticancer drug, Daunorubicin (DNR), and DNA. First, rGO was synthesized chemically and characterized by using UV-Vis, TGA, FT-IR, Raman Spectroscopy and SEM techniques. Then, the quantity of rGO assembling onto the surface of PGE by passive adsorption was optimized. The electrochemical behavior of rGO PGEs was examined by cyclic voltammetry (CV). rGO-PGEs were then utilized for electrochemical monitoring of surface-confined interaction between DNR and DNA using differential pulse voltammetry (DPV) technique. Additionally, voltammetric results were complemented with electrochemical impedance spectroscopy (EIS) technique. Electrochemical monitoring of DNR and DNA was resulted with satisfying detection limits 0.55 mu M and 2.71 mu g/mL, respectively.Öğe Hybrid nanoflowers modified pencil graphite electrodes developed for electrochemical monitoring of interaction between Mitomycin C and DNA(Elsevier, 2021) Findik, Mukerrem; Bingol, Haluk; Erdem, ArzumIn the present study, biocompatible hybrid nanoflowers (NFs) were synthesized by amino acids (glycine, L-lysine) via a simple, rapid and cost-effective methods. NFs were characterized by using FT-IR, Raman spectroscopy, XPS, SEM and EDX techniques. Modified pencil graphite electrode (PGE) surfaces with well-defined NFs were developed to electrochemical monitoring of calf thymus double stranded DNA (ctdsDNA) using differential pulse voltammetry (DPV) for the first time. SEM, EDX, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) methods were used to characterize the surfaces obtained after modification. In comparison to L-lysine NFs (LNFs-PGE), glycine NFs (GNFs-PGE) exhibited a higher sensitivity performance towards the oxidation of guanine moiety signals. The interaction time between anticancer drug Mitomycin C (MC) and ctdsDNA was aslo investigated with GNFs-PGE.Öğe Nanomaterials-Enriched Nucleic Acid-Based Biosensors(Academic Press Ltd-Elsevier Science Ltd, 2019) Zor, Erhan; Soyalp, Selen; Bingol, Haluk; Erdem, Arzum[Abstract Not Availabe]
